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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Sterilizing a non-autoclavable petri dish??
    #6846401 - 04/28/07 08:52 PM (14 years, 8 months ago)

Ok. I purchased 20 petri dishes from a UK myco site..
Guess what.. failed to mention on the site that the petri dishes weren't autoclavable plastic. WTF?! The leading UK myco site! What a farse.

10 of them melted in the PC.

I've already emailed the guy saying 'wouldn't it be a good idea to..."

Anyway.. How can i sterilize there?
What's the best way?



Cheers


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6846434 - 04/28/07 09:04 PM (14 years, 8 months ago)

They should have come in a sleeve of twenty dishes, pre sterilized. They're disposable. You don't re-sterilize. They were sterile when you opened the bag, so be sure to not open them until you're in your clean room or glovebox.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: RogerRabbit] * 1
    #6846461 - 04/28/07 09:11 PM (14 years, 8 months ago)

The bag was a flimsy bag which wasn't sterile at all.
Yeah the site sold it as sterile.. but i can assure you it wasn't.
There a couple of holes on the bag.

Just don't understand why it can't be made of autoclavable plastic.
Apparently autoclavable plastic is one of the cheapest plastics to manufacture. So by not making it autoclavable people need to keep buying more and more and they make a goddamn fortune out of it.

As you can see, its baffled me and pissed me off.

Grr!


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6846693 - 04/28/07 10:05 PM (14 years, 8 months ago)

In the ten years I've been using plastic petri dishes in pre-sterilized packs of 20, I've had only one bag that was damaged. That one was obvious because the box it came in had been damaged in transit. The disposable are worth the money for convenience. You can't sterilze for the cost of a petri dish. I get them for 8 cents each by buying a box of 500.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: RogerRabbit]
    #6849201 - 04/29/07 01:33 PM (14 years, 8 months ago)

Well.. cheapst i can find them here is 6 quid for 20. Thats $12 for 20.
That's not including the 6 quid standard postage and packaging fee..
I found 20 sterile on ebay though for 5 quid including p/p.

The ones i bought before looked like a standard bag sealer job.. i think they had probably been used then sold off to me.

For anyone in UK who reads this.. do not by from micro-science.co.uk
...just don't.


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Invisibledeucedbi9
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Registered: 10/24/06
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Loc: UK
Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6849426 - 04/29/07 03:01 PM (14 years, 8 months ago)

if they arived in an unsterile condition you should contact the co.
have you seen this one.

http://cgi.ebay.co.uk/PETRI-DISH-58-X-12-MM-PACK-OF-500_W0QQitemZ120112706479QQihZ002QQcategoryZ26414QQrdZ1QQcmdZViewItem
eBay.co.uk: PETRI DISH 58 X 12 MM PACK OF 500 (item 120112706479 end time 01-May-07 11:55:04 BST)

not on a "buy it now",but you may get lucky.


--------------------
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it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: deucedbi9]
    #6849443 - 04/29/07 03:08 PM (14 years, 8 months ago)

I would always be leery of buying anything off ebay, because the only three times I tried, I got ripped off all three times. That's enough for me, and I'll never buy anything there again. In addition, if the guy can't even spell 'separate' on his ebay page, he probably stole them.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: RogerRabbit]
    #6849516 - 04/29/07 03:36 PM (14 years, 8 months ago)

'without vents' what does that mean? That mean the bags are sealed or the petri's have no lid?.. confused me.

I ordered a 20pack from ebay.. there's a pic with sterile wrapper over it. It looks like the real sterile deal.

http://cgi.ebay.co.uk/ws/eBayISAPI.dll?ViewItem&ih=020&sspagename=STRK%3AMEWN%3AIT&viewitem=&item=300106335557&rd=1&rd=1


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6849527 - 04/29/07 03:40 PM (14 years, 8 months ago)

Non vented is what you want. No petri dish is really sealed because the lid only sits there by gravity. You'll get plenty of gas exchange that way. Be sure to order a roll of parafilm. You need to wrap them with gas permeable film after pouring/inoculating or they'll contaminate.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
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Re: Sterilizing a non-autoclavable petri dish?? [Re: RogerRabbit]
    #6849659 - 04/29/07 04:26 PM (14 years, 8 months ago)

Parafilm... i have some of that kicking around. Are you sure that's gas permeable?? I held a bit up to my mouth and couldn't breath any air, not even the smallest bit of air through it.


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OfflineCryogenicz
what?
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6849766 - 04/29/07 05:09 PM (14 years, 8 months ago)

Gas, not air.

-Graham


--------------------
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Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

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InvisibleArp
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6849862 - 04/29/07 05:32 PM (14 years, 8 months ago)

Get glass dishes. Reusable and good for the environment! :sun:


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
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Re: Sterilizing a non-autoclavable petri dish?? [Re: Arp]
    #6850057 - 04/29/07 06:19 PM (14 years, 8 months ago)

Yeah i would go for glass but i cant get them anywhere. I've checked ebay but can only find plastic. Cheers for the advice all.


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InvisibleHipsterDoofus
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6861995 - 05/02/07 10:25 AM (14 years, 8 months ago)



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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: HipsterDoofus]
    #6862015 - 05/02/07 10:39 AM (14 years, 8 months ago)

Thanks :smile:


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Offlinemushietek
melts in yourmind, not inyour hand


Registered: 04/12/07
Posts: 61
Loc: sunny scotland in a muddy...
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Re: Sterilizing a non-autoclavable petri dish?? [Re: HipsterDoofus]
    #6862017 - 05/02/07 10:40 AM (14 years, 8 months ago)

i got plastic dishes from ebay they were fine, sterile and only £0.99 for 20. check colins attic ebay shop. but anyway just wanted to say try glass universal vials. perfect for growing as with the smaller opening they are easier to transfer between without contamination. have a look at www.mycoweb.com for useage method. much better results than petri dishes for me anyway.
also i heard damp corrugated cardboard is a better medium than agar as less chance of contamination there too. both used together should give 100% contamination free 100% of the time. that will be my next experiment.


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: mushietek] * 1
    #6862074 - 05/02/07 11:07 AM (14 years, 8 months ago)

Nothing gives 100% success except perfect sterile procedures, and cardboard is no agar substitute.

If you're going to isloate strains, you'll need a LOT of petri dishes, so forget the glass ones. They're not better for the environment than plastic disposable. It takes soap to wash them, and fossil fuel energy to sterilize them. They're also heavy, slippery and breakable. Unless you have a sterile lab for the pressure cooker to cool down in, they'll be contaminated even before you pour the agar. When you're talking about needing a hundred or more to follow through a strain isolation series, that's a few hundred dollars worth of glass dishes, or ten dollars worth of pre-sterilized plastic ones. I speak from many years of experience here people. Forget the glass dishes if you're planning any more than simply germinating spores to transfer to grains. If you're seriously into doing mycology expermentation, you'll need far too many petri dishes to mess with expensive and breakable glass.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: mushietek]
    #6862088 - 05/02/07 11:12 AM (14 years, 8 months ago)

Not quite 100% :wink: but.. i get your drift.

I just received 20 sterile petri dishes from ebay today. Guess what...?
I ripped open the flimsy packaging to find that i'd burst a small hole in the bag.  Not only that, but half of the petris where all broken.

that link you gave takes to a strange business site which has nothing to do with shrooms. 

Anyways.. thanks for the tips.
I have been wanting to do the cardboard thing for a while now.

..nice day today here in Scotland eh ? :wink:


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Offlinepsilosibling

Registered: 04/14/07
Posts: 449
Last seen: 8 years, 11 months
Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling]
    #6862099 - 05/02/07 11:16 AM (14 years, 8 months ago)

Also on that note.. i got an email from a Uk myco site saying he can get me glass petri dishes for a Tenner. Far far too expensive for me. Thats like $20 for one glass dish. Plastic is better, two unlucky attempts at getting them though so far..

Petri has something against me or something.


Edited by psilosibling (05/02/07 11:17 AM)


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OfflineRogerRabbitM
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Re: Sterilizing a non-autoclavable petri dish?? [Re: psilosibling] * 1
    #6862471 - 05/02/07 01:00 PM (14 years, 8 months ago)

You need to order them from a scientific supply company in a box of 500. There will be 25 sleeves of 20 pre-sterilized dishes. By ordering like that, I've never had more than a handfull of broken dishes, and those were mostly just cracked along the edges, and were still usable, since all dishes get wrapped with parafilm anyway.

It took about 30 seconds on Google to find http://www.fisher.co.uk/ and they have the 90mm X 15mm polystyrine petri dishes I use in boxes of 600 for 48 pounds. Use the search feature on their website to find them.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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