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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlinephishyfriend
newbie
Registered: 05/14/02
Posts: 34
Loc: new england
Last seen: 21 years, 5 months
how do you clone without a petri dish
    #678098 - 06/14/02 08:52 AM (21 years, 5 months ago)

i was wondering excatly how to clone without a petri dish.can any one point me in the right direction. i need somthing with exact measurements im using a quart jar.i was wondering if i could get a good link or if someone knows and has tried it let me know. thanks


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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: how do you clone without a petri dish [Re: phishyfriend]
    #678106 - 06/14/02 08:54 AM (21 years, 5 months ago)

You can use 1/2pint jars to hold agar in like a petri dish. If you get the small buy wide (salmon) 1/2pint jars they have more surface area to work with. I dont know much abot cloning using liquid cultures (i've always thought agar works better).


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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 9 days
Re: how do you clone without a petri dish [Re: phishyfriend]
    #678145 - 06/14/02 09:15 AM (21 years, 5 months ago)

im trying cloning right now, still dont know if its gonna work yet, ill let ya know in a few days.

what i did was i took my biggest mushroom, cut the cap off, and put the stem in a 1.5 hydrogen poroxide mix and then put that into my blender, then i blended it up a bit untill it was just a foamy slurry and then i sucked it up into a syringe and inoculated pf jars... stil waiting for first signs of growth but i did it 20hr. ago....still too early to tell..


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blasted holes into night until she bled sunshine


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: how do you clone without a petri dish [Re: Alpheratus]
    #678818 - 06/14/02 03:24 PM (21 years, 5 months ago)

Cut a piece of tissue from inside the mushroom, dip in h202 and then place in a sterile solution of malt extract and water.


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InvisibleLeaveMeAlone
Stranger

Registered: 04/19/02
Posts: 1,407
Re: how do you clone without a petri dish [Re: Zen Peddler]
    #678898 - 06/14/02 03:50 PM (21 years, 5 months ago)

In reply to:

malt extract and water.



or you can just do a search of 'honey tek' ............


good luck!

peace!


--------------------
Love.


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InvisibleMushroom_Madness
enthusiast
Registered: 01/10/02
Posts: 261
Loc: Quaint New England Villag...
Re: how do you clone without a petri dish [Re: phishyfriend]
    #680193 - 06/15/02 11:39 AM (21 years, 5 months ago)

"Poster: phishyfriend
Subject: Re: how do you clone without a petri dish

i was wondering excatly how to clone without a petri dish.can any one point me in the right direction. i need somthing with exact measurements im using a quart jar.i was wondering if i could get a good link or if someone knows and has tried it let me know. thanks'

My friend, our Dynamic Spore-a-Nator will work for cloning. You simply fill it with sterile Agar/Water and then place the mushroom tissue through the laboratory access port, seal and store.

I hope this helps you with your research!


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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