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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Everyone Should Clone or Isolate Mycelium
    #6828285 -

I have finally seen my first Double Tub display really diverse characteristics in its genetics. I notice at least 3 different varying genetics in one tub. This is from a Multispore LC. I thought they would all combine into 1, but i guess not. I have blank spots in my casing layer because of a myc battle.

My recommendation, isolate mycelium or clone it.

-LK

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Re: Everyone Should Clone or Isolate Mycelium [Re: Liquidkick]
    #6828434 -

:thumbup:

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Re: Everyone Should Clone or Isolate Mycelium [Re: etam]
    #6828973 -

I have yet to see a mycelium battle :shitstorm:  :rockets:  I do agree that we all should start looking into isolations or cloning,I for one have yet to really get on it and still favor lc over any thing else but I do look forward to getting some of those picture perfect canopy flushes that only isolates can produce

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Re: Everyone Should Clone or Isolate Mycelium [Re: Blutjager]
    #6829092 -

i think this will be my next project.  :smile:


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Re: Everyone Should Clone or Isolate Mycelium [Re: Blutjager]
    #6830379 -

I have gotten those picture perfect canopys with multispore, its just this time around it let me down.

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Re: Everyone Should Clone or Isolate Mycelium [Re: Liquidkick]
    #6830682 -

This is true.

The first grow I've ever done in my life, yielded nice, straight, tall fruits. The problem with these guys was that they were hollow. I took prints of the best looking specimen(s). Soon, I inoculated jars with one of those prints and the results were much different. Even with the same parameters and bulksubstrate material, the second round with spores created relatively shorter, twisty (like twizzlers to a lesser degree), and notably.. they were SOLID. Stems were meaty as hell.

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Re: Everyone Should Clone or Isolate Mycelium [Re: Liquidkick]
    #6830795 -

Agreed! This is how the pros do it!:thumbup: This gives you the best control over things such as size, potency, aggressiveness of the myc as well as just how prolific your pinsets will be.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Everyone Should Clone or Isolate Mycelium [Re: hyphae]
    #6830849 -


How do you know you got the ones with the best genetic predisposition to high potency unless you clone & grow them all.
(When you are growing killer buds you can see & smell when you should clone it.)


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Edited by UnderNose (04/25/07 06:52 AM)

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Re: Everyone Should Clone or Isolate Mycelium [Re: UnderNose]
    #6831492 -

You isolate strains and save a master from each one. After the harvest and testing of each and every isolate, you go back to your well marked strain masters in the refrigerator and get out the best ones to grow, while discarding the poor performers. Properly prepared master slants will last for years in the refrigerator, allowing you to continue to grow the same isolate.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: Everyone Should Clone or Isolate Mycelium [Re: RogerRabbit]
    #6831999 -

Thanks RR Sounds thorough, Cloning, growing & testing them all, I thought that would be the only way to be sure.
Thats a lot of shrooms to test.:grin:

You must have a fridge load of slants. Which would be confusing even when marked.

Just curious.
Whats the most amount of sub strains you have gotten out of one strain. Cubes & edibles.?


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Edited by UnderNose (04/25/07 12:09 PM)

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Re: Everyone Should Clone or Isolate Mycelium [Re: UnderNose]
    #6832045 -

Every time monokaryotic mycelium mates with a compatible hyphae, a 'strain' is born. That is the definition of 'strain', not the name somebody wrote on the sporeprint. This means that one swipe of spores from a print onto agar can easily yield dozens, if not hundreds of strains.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Everyone Should Clone or Isolate Mycelium [Re: RogerRabbit]
    #6832125 -

Wait one minute...

You have to eat them to test for potency?

I thought just fruiting them and picking the strains with the best flushes and highest yields was the final step.

Is there really going to be that big of a difference in potency?

I'm not even sure how one would test for potency unless one waited 4 weeks between tests. That would take me 2 years to test my isolates. Even then, one trip from one strain can vary big time from the next trip even if it's from the same strain/flush.

Maybe I'm missing something.


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Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!

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Re: Everyone Should Clone or Isolate Mycelium [Re: RogerRabbit]
    #6832144 -

RogerRabbit said:
This means that one swipe of spores from a print onto agar can easily yield dozens, if not hundreds of strains.
RR


Thats crazy.:eek:
You may have to grow a single print 10 or more times to see all it has to offer.
Agar, agar everywhere, Petri dishes in the sink.

Do you think that some strains wouldn't appear unless agar work is done.

I mean when a LC or multispore inoculation are done the number of strains would go down as all the strains compete.
You may be left with the fastest most aggressive strains, but not necessarily the most potent.

P.S.
Excuse my poor grasp of some terminology.


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Re: Everyone Should Clone or Isolate Mycelium [Re: UnderNose]
    #6832196 -

That's correct. I've used up an entire box of 500 petri dishes before on one sproreprint. All strains have to be tested for the qualities you seek. I'll leave the 'how' up to you.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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