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Registered: 06/02/01
Posts: 117
Last seen: 15 years, 9 days
Isolates vs Clones
    #1497193 - 04/27/03 02:48 AM (15 years, 29 days ago)

I dabble now and again in the hobby. I have a problem with the fact that my only fruits produced thus far have been from multi-spore innoculations. I enjoy doing agar work (isolations from multispore) but can not get an agar xfer to fruit (also have problems getting agar to colonize jars). Should I shitcan the isolate agar work and go for cloning? BTW, can clones be taken from sclerotia? Having such bad luck w/fuiting that this might be my solution.

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Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
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Re: Isolates vs Clones [Re: homebrew]
    #1497242 - 04/27/03 03:26 AM (15 years, 29 days ago)

You'll have to use agar for cloning anyway, so I think that's not a solution to your problem. You had problems in colonizing jars because of contamination, not because of difference in using isolates or clones. Try to improve you sterile procedures & use peroxide (H2O2).
Yes you can clone sclerotia too.

Edited by zeronio (04/27/03 03:29 AM)

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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