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Honey LC first try
    #6717152 -

Hello

Well after shooting up 4 jars of WBS (multi spore) and failing. (Wet spot) I have 2.5ml of spore solution left over.

I went out and bought organic honey today to make some LC with the left over spores.

Right now I'm boiling all my jars and supply's so that i start out on the right foot.

After much reading my plan of attack is as follows:
*1qt jar is to be used
*lid has 2 holes both sealed with silicon to create a vacuum
*500ml of water will be used to 15ml of honey
*Piece of glass will be inserted before PC
*PC for 20 min as i only have a 12psi pressure cooker
*Let PC loose pressure on its own

And thats about as far as i made it so far.

How long after the pressure cooker looses pressure should i wait before i inoculate?

Is there any issue with having both holes sealed? I plan on getting another syringe filling it with Polly fill to relieve pressure when i go to extract LC.

And last but not least am i missing anything? Any and all suggestions are greatly needed.

Thank You

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Re: Honey LC first try [Re: Crazykappa]
    #6724915 -

Well my LC is on its way. I just hope i did it right.

There are no signs of over cooking so i would assume i did everything right thus far.

Is there any problem with having both holes sealed? What about FAE?

Thanks

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Re: Honey LC first try [Re: Crazykappa]
    #6729695 -

I have basically the same kind of LC's. After you inoculate with the spores keep adding sterile air with a syringe until there is no more vacuum. Then, after that just inject 10cc's of sterile air every week into it.

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Re: Honey LC first try [Re: MkUltra]
    #6729753 -

so how long DID you wait after PCing to inoculate? you let it cool all the way down first, right?

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Re: Honey LC first try [Re: ApJunkie]
    #6731530 -

Hello

Just a Lil update my jar is looking like a snow globe:) When do i know when its done?

Also about sterilizing syringes? How do i do it? any good teks out there?

So add sterile air to the jar? Again how do you sterilize my used syringe with air in it?


Yes i let it cool all the way down before "knocking it up"

Thank you

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Re: Honey LC first try [Re: Crazykappa]
    #6731550 -

Vacuums in LC jars, are a major cause of contamination.
As, a vacuum often sucks in contaminates.:eek:
1 hole with a port, another hole, with filter, in the lid.
That way, you don't have to worry about air/ports, or vacuums.
:grin:

With the right lid & technique.
You can even forgo PC'ing.
And, use a microwave, instead.


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Re: Honey LC first try [Re: agar]
    #6731563 -

Hello

Thanks for the notice how do i create a sterile syringe to put some sterile air in there?

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Re: Honey LC first try [Re: Crazykappa]
    #6731586 -







You can stuff a syringe with polyfill.
Then, PC the syringe, wrapped in a/foil.
But, that airport method is not great.

Better to simply start over.
With a lid, with a gas exchange filter & port.
You can make a filter;
with a small square of tyvek.
Held in place, over the hole, with micropore tape.


--------------------

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Re: Honey LC first try [Re: agar]
    #6731611 -

Starting over is not an option i shot it up with the last of my spore solution. So far it looks like healthy growth so i have to run with it.

But yeah i would love to get some of them lids

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Re: Honey LC first try [Re: Crazykappa]
    #6731635 -

Growth in you jar will eventual stall.
Without some gas exchange provision.
You are going to have to cobble together some sort;
of sterile airport filter.
Otherwise, you will play hell;
Getting any LC out of your jar.

3ml syringe, polyfill stuffed.
Try building 1 of these.
(if you have a spare syringe around)
Then, PC it, wrapped in a/foil.


--------------------

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Re: Honey LC first try [Re: agar]
    #6731790 -

If this method doesn't work for you, I suggest you check out http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1

As pretty close to full proof as one can get. Good luck


--------------------
To be shaken out of the ruts of ordinary perception, to be shown for a few timeless hours the outer and the inner world, not as they appear to an animal obsessed with survival or to a human being obsessed with words and notions, but as they are apprehended, directly and unconditionally, by Mind at Large--this is an experience of inestimable value to everyone and especially to the intellectual. --Aldous Huxley

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Re: Honey LC first try [Re: agar]
    #6731794 -

Ok ill give that a shot

When i PC it in a/foil do i just set it in the water or should i place it in a jar or something so the water does not touch it.

And do i PC it will the polyfill in the syringe?

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Re: Honey LC first try [Re: Crazykappa]
    #6731820 -

Stuff syringe with polyfill before PC'ing.
Wrap in a/foil.
Place in a jar with no lid.
Cover jar top with a/foil, loosely.
So syringe doesn't get water logged.


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Re: Honey LC first try [Re: Crazykappa]
    #6731918 -

Crazykappa said:
Starting over is not an option i shot it up with the last of my spore solution. So far it looks like healthy growth so i have to run with it.

But yeah i would love to get some of them lids


no disrespect to agar,but they'd be an absolute waste of money imo.
If you had put some poli-fill in one of the holes,assuming you use tyvek between jar and lid,you'd have been fine.

as you have a pc the tek posted by "guitarmonkus" would entail lot of unnecessary poncing about.
that tek is designed for people going the pf route because they don't yet own a pc.


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule

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Re: Honey LC first try [Re: agar]
    #6731919 -

Thank you very much for the info will do this tonight.

One more question all i have is the syringe that came with my spores ive been calling around locally to buy a few more do i need 18gage or can i go with smaller size?

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Re: Honey LC first try [Re: deucedbi9]
    #6731950 -

deucedbi9 said:
Crazykappa said:
Starting over is not an option i shot it up with the last of my spore solution. So far it looks like healthy growth so i have to run with it.

But yeah i would love to get some of them lids


no disrespect to agar,but they'd be an absolute waste of money imo.
If you had put some poli-fill in one of the holes,assuming you use tyvek between jar and lid,you'd have been fine.

as you have a pc the tek posted by "guitarmonkus" would entail lot of unnecessary poncing about.
that tek is designed for people going the pf route because they don't yet own a pc.


You're right about the tek being for noobs. I do own a PC, but I find that this tek is beneficial to me anyway. I dislike dealing with my pressure cooker, and find it somewhat unreliable (mostly because I haven't used it yet where I didn't get a vacuum after cooling. Every time I have to pry the lid off with great effort with a large screwdriver. No doubt a user problem, but nevertheless something I have not figured out quite yet). But to each his own.


--------------------
To be shaken out of the ruts of ordinary perception, to be shown for a few timeless hours the outer and the inner world, not as they appear to an animal obsessed with survival or to a human being obsessed with words and notions, but as they are apprehended, directly and unconditionally, by Mind at Large--this is an experience of inestimable value to everyone and especially to the intellectual. --Aldous Huxley

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Re: Honey LC first try [Re: deucedbi9]
    #6731971 -

Quote:
no disrespect to agar,but they'd be an absolute waste of money imo. If you had put some poli-fill in one of the holes,assuming you use tyvek between jar and lid,you'd have been fine.


None taken.:tongue:

Next time you make an LC, by your method.
Try turning the jar of liquid upside down.
Then, shake the hell out of it.:eek:
And, see how long the filter remains dry & content uncontaminated.


Certainly, the average LC filter doesn't get abused, like that.
These can take it.
I have NEVER seen another, that could.


--------------------

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Re: Honey LC first try [Re: agar]
    #6733133 -

Well my LC no longer has the whole negative pressure thing going on.

I used a tek i read for sterile air by sucking up air in a open flame. I hope it works...

Other then that the LC is looking great lots of growth in there :smile:

Now how do i go about sterilizing the syringe to fill with LC? I know PCing it but how? Would i break it down into all parts place in jar with a/foil over it all?

Thanks Guys

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Re: Honey LC first try [Re: Crazykappa]
    #7605336 -

Crazykappa said:
Well my LC no longer has the whole negative pressure thing going on.

I used a tek i read for sterile air by sucking up air in a open flame. I hope it works...

Other then that the LC is looking great lots of growth in there :smile:

Now how do i go about sterilizing the syringe to fill with LC? I know PCing it but how? Would i break it down into all parts place in jar with a/foil over it all?

Thanks Guys


I too am interested in this, what's the best method to sterilize a syringe?

EDIT: oops, didn't realize this thread has been dead for 8 months...

Edited by Psilocin Dreams (11/07/07 01:57 AM)

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