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OfflineMagicius
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giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe
    #7861271 - 01/10/08 07:45 PM (16 years, 22 days ago)

Okay so that is not the main reason for this message... perhaps I'll change the title...

http://www.tevo.co.za/Product.aspx?intProdId=100001039


Edited by Magicius (01/30/08 09:20 AM)


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Offlinemakaveli8x8
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #7861333 - 01/10/08 07:58 PM (16 years, 22 days ago)

well i only read the first 2 paragraphs but i think i got the jist of what your trying to do.

Two problems the way i see it, one i think the reason people use grains for spawn is to get the mycelium energy and to be healthy for when its used to spawn.

using just verm its going to use all its energy "searching", when you spawn it, it will prob be weak.


the only positive thing i see about your idea is that its not going to contam very easy...this may be the only reason why it might colonize faster/easier


--------------------
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Offlineveda_sticks
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: makaveli8x8]
    #7863573 - 01/11/08 03:50 AM (16 years, 21 days ago)

so even if this does work, what are you going to do with 2 litres of colinised verm?

I'd say that it will still be able to contam. Theres plenty of water, and a little bit of food from the honey.

Bacteria can grow on water without what we would consider food.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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OfflineMagicius
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: makaveli8x8]
    #7863642 - 01/11/08 05:09 AM (16 years, 21 days ago)

I guess I could buy a big bag of dogfood, crumble it, and mix it in with the verm... I mean the 3 % was enough for the mycelium to develop inside the LC... true i don't want them to be weak. I want them to be lean and athletic.

I don't want my hyphae sitting around digesting, when they need to be getting excercise =] But I agree athletes do need energy if they burn a lot... sigh. metaphors are such a pain in the ass.

i guess you are right. i mean you need food to do excercise, and move around. so i guess the mycelium does too... I just don't want them to behave like cows, ruminating on all the nutes, instead of moving around lookin for pastures with at least one of their four stomachs full...

thanks for the response. m glad someone's out there. a hoo ahooo echo oh oh?

:smile: and its very encouraging that you didnt  think i was totally nuts for well using so much LC the underlying tone of your post does suggest that it isnt too far fetched.

I mean the largest LC inoculation I had previously haerd of was Blue Helix with 140 ml. But I just realised, since I've not ONCe had a successful bag grow (I don't have a bag sealer, don't flame my needles, needles fall out of the plastic thingy attaching it to the syringe - old needles), and also bad strain that is retarded slow...

so i just thought yz more lc will do the trick.

i mean i once had about 100 ml of wbs/poo and injected 30 ml Lc, with a nest of coco coir at the bottom of the tiny lil jars.
they grew pretty quick... it should work on large scale as long obviously as the quantity LC / verm moisture / volume ratios balance each other out...

Over analyzing -(s)Tool...


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OfflineMagicius
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #7863702 - 01/11/08 06:01 AM (16 years, 21 days ago)

yz vedasticks, but like i said i tested the quantities. the moisture is just perfect w/ 300 ml lc and 2 liter dry verm...

I donno i don think the backterriers will be able to get a grip, I mean thats sucha large volume of lc, they won't get a chance on any corner of any bit of medium sized vermiculite, that has been carefully measured so as to allow for absence of sogginess or run off, allowing the immigrating hyphae the perfect homeland, at the perfect moisture content that would prevent any run off or slower growth from too much moisture...

I'm a definitely post pics and results about this,a log tek. IF, and *only if* it totally works... =]


---
of course for safety's sake I added 40 ml of water injected into the bottom of the bag where the layer of coco coir is to act as a buffer for any bulk liquid presence (it doesnt cause any pooling, i just add that lil bit of moisture coz I don't know if totally dry vermiculite will burn, so I jus add like that 40 ml to the bottom of the bag so it can steam through the verm- i mean i donno if the bits can burn or melt onto each other if they arent moist...?; its worked wonder for me, doing that with coir (stuffin it at the bottom to compensate for possible extraneous lc presence...

but again, I checked the moisture content of the verm (2l) w/ 300 ml water. It wasn't soggy at all. In fact you had to squeeze pretty hard to get a slight trickle out. I'd say its on the near side of perfect, moist but not too moist, and no runoff. I don't know why I didn't test the ratio's before...

oh yeah. I didn't have a 300 ml syringe... =]...

problem now is though I am too tired to put a needle in that huge thing... i just have to add a mounting plastic otherwise the needle wont be stable enough....

i have a wonderful dictyophora indusatia I'm trying to grow, the 'spontaneous orgasm mushroom. No one has ever artifically cultivated them...

if somehow i can trigger primordia, I'm set for life =]

I can't believe I enjoy experimenting with mushroom cultivation techniques...a year ago I would have considered it arduous labour... now it actually seems to have gotten me attentive and interested in simplifying tekology.


Edited by Magicius (01/11/08 03:45 PM)


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OfflineNibin
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #7863709 - 01/11/08 06:04 AM (16 years, 21 days ago)

I bet that if you keep doing stuff in a half assed way it is going to fail miserably.

PS: It's as if you were trying to grow the famously impossible hydroponic shrooms.


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Offlineabesh
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #7863713 - 01/11/08 06:07 AM (16 years, 21 days ago)

Are you saying you want to mix your LC with verm, and hope that that turns into spawn that you can use. If that is what you are trying to do ... I do not think it will work (I may be wrong) the LC will not really colonize the verm as there is no food.

And also... why would you want to do it in a dirty environment? It is not really that hard to do it in a sterile environment. You could have a glove box, alcohol, spray bottle, gloves, etc for less than $20.

I would suggest having a successful grow, and then trying to modify things when you have a better understanding.

You may be on to something... but to me it just seems counter-intuitive, and a wast of materials, money, and time.


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OfflineFraggin
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: abesh]
    #7864228 - 01/11/08 10:09 AM (16 years, 21 days ago)

If you wanna half ass it, find a pile of horse shit, put in in your back yard, innoc it with lc, and cover it with straw and turn a 5 gallon bucket upside down and place it on top of it.

Let me know if it works ...  :grin:


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OfflineMagicius
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Fraggin]
    #7865750 - 01/11/08 04:22 PM (16 years, 21 days ago)

FEllas fellas!

BUT WHAT DO YOU THINK OF THE GIANT SYRINGE? aint it coo/

I've been messin around with this stuff for what, 3-4 years...
trust me...

I've just simplified the maths. I mean Blue Helix has proved that you can inject 140 ml into a spawn bag and have a harvest 25 days after creating lc from spore, by compensating with dry verm in the suybstrate..

The reason why I half ass is well, its not really half ass, I mean i alcohol swab my rtv ports and all, now i got a bunsen burner today, i can flame the needle totally, I don't have to do stupid crazy lazy drunk shit like just opening the lid and "hoping" contams won't land...

But in a sense you are right I am doing my best to half ass it, dude/. guys... Its like maths. factorize factorize. Baby steps Bob. I'm not trying to Salsa, here =].

So you are right i do use alchol swab, correct. What I don't use is a glove box, coz in fact, i dont have a table, I have a huge safe, my dad's lawyer's safe, its so damn heavy i cant fuckin move it; trust me, we've tried... lol. we even hired some er... industrial cutting devices, but to no avail... hahaha. honestly, he's like an advocate, they're worth fuckin millions. maybe theres some skulls in it, i donno. or guns. I think there's guns but I wont know until civil war breaks out. but what I was saying is, I work on a safe, and its really a mean safe, coz its JUST *JUST* too fucking low to cooperate with my glove box, which I do have, but have divorced with no hope of remarrying the damn thing; bending over that god damn safe, looking into that glovebox gave me the worst back ache ever. Also I felt my arm movements greatly hampered by the thing, it was just stifling. Although honestly I felt marvellously clever; I took black rubbish bags, punched holes in the corners, and used them for sleeves, up to my shoulders. Then the corner where the hole and my hand went through is, i tuck the bag under surgical gloves, so I really looked like a turkey when I started working with my glovebox. It was just too uncomfortable to move shit around inside it... anyways, i dont need it coz i whipe the innoc ports with alcohol, and stick the needle in while still burning hot, melting the silicon port, which is fine, it still glooped over the hole. That way there is no exposure , and the culture remains isolated, and thus, pure ....

But perhaps i shouldnt have mentioned that when I'm depressed and lose sight of the big picture of a successful hobbyist, i use syringes whose needles keep falling out and opened lc jar lids coz it was annoying to syfen (syphon?) lc out , but I'm superglueing my needles, no im not buying new syringes, needles...

but like i said amidst all my miserable cultivation distasters, the least (the LEAST: ha!) was drinking an entire bottle of whiskey, and then being so drunk and full of gusto that I thought I could just 'grow mushrooms'; i dont even remember if I was making lc; or spawning or wtf... but coz i couldnt see contams, they wherent there. until a few weeks later. man that was dumb.

one half assed success is definitely the use of coir bedding at the bottom of a jar and/ or supplementation with dry verm, so that you can inject like what, 10-300 times more LC than you normally would, to speed up colonization. Ive succeeded at it in small scale. It is idealogically, and logically reasonable to assume that compensation for additional moisture can also be exponential...

I mean i injected 30 ml lc into so little substrate, it was ridiculous, into like a 100 ml wbs/poo, ontop of a lil coir bed, which suspended the wbs/poo above the 'pool' of excess lc, which stayed amidst the coir. when i looked again at those jars, the moisture was gone, the coir was totally colonized, and shrooms had started pinning before I had even noticed the damn things had colonized.
Yes, there where some contams; but thats not because of excess lc; ive proven it is a technique in itself to use excess lc, for faster colonization, when compensated for with

a)coir bedding at the bottom to suspend the medium above any possible water pooling
b) quantified dry verm that will sponge up all the 'exces'' lc, thereby attaining perfect moisture upon inoculation, i.e in a way the jars aren't totally prepped (like soaking grain before PCing... ) but rather, the inoculation is the final step of the PREP.... =]

but i had some contams coz my needles fell out the syringes (too much boiling to sterilize, i use em for what almost 2 years now...)

ive STILL been too lazy to superglue them... u know its little things like that that has really made me half ass, i think u r right, FRaggin, Nibin... and also the damn lighter to flame the needle that kept running out of fuckin gas so that I ended up only flaming like in many cases, the needle for like 2 in 20 jars. And also when the needle fell out the syringe, i just stuck it back in with my hand. Still I had pretty good success rates. But I mean that's trivial shit.

But its 2008. I've turned a new page.

From now on, my HALF ASSED TEK will be done properly; and guys, fellas, I *WILL* post you on it so that if I *AM* right , that i can rub your noses in it =]

of course if I'm wrong about it... then i will rub my own nose in it ...
but my reason for wanting jus colonized vermiculite... to spawn to pasteurized STRAW, bulk. Thing is, I can't liquid inoculate straw.

mmm. i guess i could just shoot lc into pastuerized verm/poo mix, too. But i wanna really C if its possible to make LC&verm only spawn. I mean yz, there is food still in the lc liquid... and if i shoot that much lc into the bag, well, the mycelium will be all over the place. they will not have to colonize very far before its complete at that rate, so they wont need a lot of food i think. i dunno.

yz i talk the talk but do i walk the walk; er well tomorrow, yz.

My Goal: Growing Mushrooms with the Least Effort and the Least Money.

I consider laziness a virtue; for is it not economy of action?

I guess spawn to straw or something, then I will be using gloves again, but as long as its invitro lc transfers, I should technically be able to dust my hands with trichoderma and still have 100% success rates, coz the flame should sterilize the needle, if its hot when goin in its coo, and with a swab at hand, and also with gas exchange filters worth their weight (...), it should be granted that amidst invitro lc transfers, one should be able to be a trichoderma mould and still be able to grow pure mushrooms.

Every successful innovation I've ever had stems not from my wanting to be thorough, but from my being HALF assed, wanting an easier way. Counter intuitive, yes, some might say I learned the hard way; yes i did. I made all the damn mistakes. In fact some of them I was aware of doing while i was making them; but the real mistakes are the ones you didn't know about, and learn about later.

I mean if you know somethings wrong, and you do it still, it isn't really a mistake coz you did it on purpose, it was no accident.

Man o man, i jus smoked far too much weed. at least i din go into explaining how I would model trees and plants in povray (3d graphics engine)... sry.

i do lose it sometimes.


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InvisibleGreen420Thang
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #7865763 - 01/11/08 04:25 PM (16 years, 21 days ago)

Magicus, the only thing there is to do, is for you to try this out. Talk is cheap, proof is in the puddin.


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OfflineMYSTIQUE
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Green420Thang]
    #7865791 - 01/11/08 04:30 PM (16 years, 21 days ago)



--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


Edited by MYSTIQUE (01/11/08 04:35 PM)


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OfflineMagicius
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DEFINITION OF SPAWN VS SUBSTRATE [Re: Magicius]
    #7865884 - 01/11/08 04:47 PM (16 years, 21 days ago)

in any event 300 ml into 2 liters didnt work.

search for some of blackout's grain grows, search for 108 ml... =] he';s got some grand experience, finely logged regarding innoculating with large volumes of lc (very high ratios into prepared, dried grains), with great success, too =]. Personally I think blackout is a genius for pulling that off for us all


Edited by Magicius (03/01/08 04:27 AM)


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OfflineMycopath666
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Re: giant syringe - Innoculating 2 liters of verm with 300 ml LC 1 shot giant syringe [Re: Magicius]
    #17399325 - 12/15/12 11:19 PM (11 years, 1 month ago)

gnarly


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