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OfflineInjectTruth
Wasting my Time,Waiting for theEnd
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Registered: 10/05/03
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Liquid Culture... Spores or Contamination?
    #6328477 - 12/02/06 08:39 PM (17 years, 3 months ago)

I recently inoculated a bunch of karo jars with a variety of cube strains from TLG.

Only one has done anything at all. It looks fine, except for some purple specks deep inside the mycelia. Are these ungerminated spores that "condensed" on the mycelia? I dont recall seeing them upon inoculation.

I am really beginning to question the viability of these syringes.












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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
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Re: Liquid Culture... Spores or Contamination? [Re: InjectTruth]
    #6329558 - 12/03/06 04:00 AM (17 years, 3 months ago)

from here, it would appear you have a beautifully healthy culture under way.

those specs may very well be remnants of spore "shells" and/or clumps of ungerminated/unviable spores...

at any rate, usually when you have a contaminate problem in an LC from the start, it won't happen at the same time as healthy growth.  and i see healthy growth.  that's good news :smile:

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OfflineInjectTruth
Wasting my Time,Waiting for theEnd
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Registered: 10/05/03
Posts: 778
Loc: New Jerusalem
Last seen: 1 month, 2 days
Re: Liquid Culture... Spores or Contamination? [Re: creamcorn]
    #6329970 - 12/03/06 11:13 AM (17 years, 3 months ago)

Thank you very much. Do you think I should make syringes out of it now, or allow it to mature a little more?


--------------------
On a personal level, Freaking Out is a process whereby an
individual casts off outmoded and restricting standars of
thinking, dress, and social etiquette in order to express
CREATIVELY his relationship to his immediate environment and
the social structure as a whole.

http://www.OrganicPharming.com - Ethno Shopping Portal

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OfflineQuake3
Total Carbohydrate
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Registered: 08/31/06
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Re: Liquid Culture... Spores or Contamination? [Re: InjectTruth]
    #6332525 - 12/04/06 10:58 AM (17 years, 3 months ago)

You can make a syringe now, but be sure to leave some mycelium in there so it will continue to grow. Try breaking up the mycelium before aspirating into a syringe.

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OfflineGrogan
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Registered: 05/20/03
Posts: 1,146
Last seen: 8 years, 10 months
Re: Liquid Culture... Spores or Contamination? [Re: Quake3]
    #6332880 - 12/04/06 01:05 PM (17 years, 3 months ago)

Very very good looking lc growth there no contams!


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"Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL

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