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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Contam on maitake preparing to fruit
    #6282292 -

I have 3 bricks in gallon ziplock bags made from pine blend wood pellets + WBS. They are fully colonized (what a great minty scent maitake gives off when eating pine!) and they are on a light timer to induce fruiting. One is now growing out of the bag rather quickly. (I'll post pics later, I'm at work now.)

This morning before work when I checked, I noticed that growth has really accelerated but there was a small spot of green mold :frown: in the middle, probably trich. It was growing directly on the grifola mycelium. I alcohol-sterilized a scalpel and excised the quarter-size portion with visible mold, then sprayed the area with peroxide. If successful, this will be my first harvest of mushroom I've grown myself, and my first time trying maitake. My family is really looking forward to a possible thanksgiving treat :smile:. So I really want to save this one if I can.

All suggestions are appreciated!

Here are some more details of my setup:

My mushroom room is a small, windowless room. An air cleaner runs constantly. One ultrasonic mister and one steam humidifier run constantly. Light and heat are on a 12 hour timer. Light is from a small $10 flourescent from Walmart, directly over the maitake. Daytime temp is 85, night temp 75. Humidity fluctuates between 55% and 80%, but usually maintained between 70% and 75%. Humidity is slightly higher inside the aquarium where the maitake are.

The maitake are in a substrate of cooked pine blend pellets + WBS in gallon size ziploc bags. After full colonization, the sealed bags were opened to increase air exchange and placed on the top shelf of a wire rack. A glass aquarium was placed over the top of the bags to prevent contams from settling from above. The maitake are all still in the original bags, and I'm also wondering if the bags need to be removed to allow the sweat and condensation to drain off.

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Re: Contam on maitake preparing to fruit [Re: pokermush]
    #6284389 -

Your tempatures seem pretty high for maitake.
The best time to find them outside is when the
daytime temps are in the 60 and 70's and the night
time tempatures are in the low 50's.
:thumbup: Congradulations on getting fruits on
blocks.

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Re: Contam on maitake preparing to fruit [Re: falcon]
    #6284427 -

Another alternative is to wash the substrate under the faucet every few days to help prevent trichoderma.

I've found that trich on wood based substrates is more of a surface phenomena that can be easily removed. I use a q-tip dipped in salt paste. Gently twist the q-tip to remove the green spores first, using more than one q-tip if necessary. Once everything you can see is removed, get a fresh q-tip dipped in the salt paste and vigorously rub the area about double the size of the trich. This changes the Ph so drastically, it shocks the trichodema into not recovering.

It only takes a few drops of water to turn 1/4 of a shotglass of salt into a good paste that sticks, but doesn't run.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Contam on maitake preparing to fruit [Re: RogerRabbit]
    #6284735 -

Thanks for the tips falcon and RR. The salt idea is great!

Here are a couple pics:


You can see the hole and "scar" in this one where I removed the mold:

Not quite fruiting yet, but it looks like it wants to, and is now growing very fast.

So, do you think I would be better off removing the blocks from the bags and putting them in a semi-transparent tub in a different room at 65 to 75 degrees?

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Re: Contam on maitake preparing to fruit [Re: pokermush]
    #6285521 -

In my experience Maitake wants massive air exchange and low temperatures. I doubt you could get that in a tub. You might try putting them outdoors for a few days if the temperatures are right in your area. They seem to want a good cold shock for a few days before they fruit.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Contam on maitake preparing to fruit [Re: RogerRabbit]
    #6288227 -

This changes the Ph so drastically, it shocks the trichodema into not recovering.

More likely the hypertonic environment than pH.

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Re: Contam on maitake preparing to fruit [Re: Pinback]
    #6293647 -

what's salt paste? where is generically sold?

i have a G.frondosa bag colonized in 15 days. i've seen that this mushroom create a lot of secretion(i can see also the pokermush bags with secretions).It stinks a bit! Is normal?

today i've showered with cold water the G.frondosa block and putted to fruit into the green house..if pins doens't start into 3-4 days i'll try to cold shock it.

is possible to stimulate the pinning without cold shock?

thank you all.

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Re: Contam on maitake preparing to fruit [Re: Pinback]
    #6293658 -

Pinback said:
This changes the Ph so drastically, it shocks the trichodema into not recovering.

More likely the hypertonic environment than pH.


OK, please enlighten the boorish on this site. 

What does "hypertonic" mean, and what does that mean for environmental factors for cultivation?  Thanks! :laugh:


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!

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Re: Contam on maitake preparing to fruit [Re: pokermush]
    #6295581 -

OK I pulled the blocks out of the bags, trimmed protruding flaps of mycelia, washed thoroughly, then placed in a bin with an ultrasonic mister. The mister is 1 hour on 1 hour off.



Here are the bigger pieces I trimmed from the blocks:

If you're wondering about the 3rd block... it had too much trapped moisture / not enough air flow in the bottom of the bag, and as a result the inside of the block was not well colonized. I removed it anyway and washed it, then placed it back under the aquarium to see if it will be OK.

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Re: Contam on maitake preparing to fruit [Re: GnuBobo]
    #6295797 -

In hypertonic environment with high concentration of salt, sugars or other solubles, osmosis takes water out of the organism, making survival difficult. Googling for "hypertonic" gives this as first link...

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Re: Contam on maitake preparing to fruit [Re: rip]
    #6297392 -

That is a nice and concise explanation.  :thumbup:

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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