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Offlinethe spiral
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Agar plates & ciprofloxacin/penicillin
    #5970409 - 08/16/06 08:22 PM (15 years, 2 months ago)

Hey, and thanks for reading my post.

A friend of a friend is going to be culturing cubensis on agar plates, and wants to add antibiotic to his malt agar solution in order to prevent bacterial contamination. He currently has in his posession about a dozen ciprofloxacin pills, and he thinks he might be able to dig up penicillin somewhere.

My friend of a friend was wondering if it would work to simply use some powderized cipro pills to add to his agar solution (before sterilizing), and how much to use (milligrams of cipro per liter of malt agar solution, for example)

Thanks for your input! My friend much appreciates your help


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Offlinecurenado
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Re: Agar plates & ciprofloxacin/penicillin [Re: the spiral]
    #5973102 - 08/17/06 04:33 PM (15 years, 2 months ago)

Typically gentamycin is used in agars and in very small amounts.

Suntzu has said:
<<Ampicillin is cheap; I use 50 ug/mL. Tetracycline, I don't know what is optimal, but 30 mg/mL works. [both post-sterilization, of course].>>

Best wishes!


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OfflineRogerRabbitM
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Re: Agar plates & ciprofloxacin/penicillin [Re: curenado]
    #5973302 - 08/17/06 05:47 PM (15 years, 2 months ago)

Gentamycin sulphate is the only antibiotic I know of that works well with agar. It is added BEFORE sterilization at the rate of 1/15 gram per liter.
RR


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OfflineWorkmanV
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Re: Agar plates & ciprofloxacin/penicillin [Re: the spiral]
    #5974159 - 08/17/06 10:15 PM (15 years, 2 months ago)

Most antibiotics are heat sensitive and are inactivated by sterilization. Gentamycin is the exception and can be added before cooking. If you wish to use most other antibiotics, they have to be added after sterilization and after the agar has cooled siginificantly (but still liquid). Since the pills are not likely sterile, you would probably be adding more contamination than you would be preventing.


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OfflineAnnoA
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Re: Agar plates & ciprofloxacin/penicillin [Re: Workman]
    #5975287 - 08/18/06 09:03 AM (15 years, 2 months ago)

Some other antibiotics that are not rendered completely useless due to sterilization are penicillin and/or stroptomycin.

See this post from 2003:
http://www.shroomery.org/forums/showflat.php/Number/1223042


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InvisiblePinback
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Re: Agar plates & ciprofloxacin/penicillin [Re: Anno]
    #5975298 - 08/18/06 09:14 AM (15 years, 2 months ago)

Chloramphenicol is also stable to high temperatures, as indicated here. Note however that it is bacteriostatic, and not bacteriocidal.


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Invisiblefastfred
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Re: Agar plates & ciprofloxacin/penicillin [Re: Pinback]
    #5988732 - 08/23/06 06:32 AM (15 years, 1 month ago)

I've had very mixed results adding random antibiotics in random amounts to liquid cultures. I don't really recommend it unless you're just experimenting.


-FF


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Invisiblemycogirl
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Re: Agar plates & ciprofloxacin/penicillin [Re: fastfred]
    #5992506 - 08/24/06 02:10 PM (15 years, 1 month ago)

Unless the antibiotic is specifically culture grade (which oral antibiotics are not), you will have variable results. The easiest way to avoid contamination is good technique.

In addition, tsk for not taking your antibiotic until it was gone. This is bad for more reasons than I have time to list.


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Offlinecurenado
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Re: Agar plates & ciprofloxacin/penicillin [Re: mycogirl]
    #6038498 - 09/07/06 03:32 PM (15 years, 1 month ago)

<<The easiest way to avoid contamination is good technique.>>

That's all we do and we do pretty good! Your tech and your clean box are your best defenses.


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