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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Is mycellium taken from a clone mean no more sectoring?
    #5808097 -

I mean lets say you did a multi-spore bulk grow and took mycelium from a large cluster that fruited MUCH earlier than the rest. No, it wasn't an uneven substrate or casing level, bye the way.

Tissue taken from a clone is close to the final stage of sectoring?

I mean, the next grow from this mycelium should have the same traits, but does it become an actual ISOLATE mycelium?

Just a thought, I'm tired.

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Re: Is mycellium taken from a clone mean no more sectoring? [Re: capnstemz]
    #5808230 -

you know...
i was about to launch into why i thought it was an isolate
but then i second guessed myself and started saying no
it was then that i realized, i have never actually sectored out a clone, and have only done so with petri to petri
so you raise a very good question that i too would like to know the answer to

im going to have to go with my second guess of no
the reason behind this...
in some of rogerrabbits pictures of his clones of pins on agar, you'll notice that one of the three in his dish has definite cottony sectors, or tomentose type growth, if you prefer (and read mushroom cultivator alot)

http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=5703541

check about halfway thru the first page, to when he actually stops teasing us, and shows us if it'll work or not. =)


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Re: Is mycellium taken from a clone mean no more sectoring? [Re: capnstemz]
    #5809169 -

RR, you're thought on this?

So, it's stil better than multi-spore, but not as good as an isolate?

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Re: Is mycellium taken from a clone mean no more sectoring? [Re: capnstemz]
    #5809203 -

I'd take several clones and see which one comes closest to the desired traits you're wanting to preserve. We know that with multispore inoculation hundreds and sometimes thousands of strains are created. I've definitely seen more than one strain manifest itself in the same fruitbody, so by cloning tissue from one of those fruits, sectoring will occur on agar.

Cloning a cluster is a quick way to get a great repeat performer, but remember if it does sector, be sure to grow out each sector to determine which one you're really after.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: Is mycellium taken from a clone mean no more sectoring? [Re: capnstemz]
    #5812173 -

So a clone has less strains in it?

Did 12 monotubs with multispore and close to none of them have the same traits, some mushroom "balls", some thin and lanky, some short and fat, some with LARGE clusters, some short and fat.

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Re: Is mycellium taken from a clone mean no more sectoring? [Re: RogerRabbit]
    #5812395 -

:thumbup: :thumbup:

and some healthy looking sectors won't grow fruits very well...

so you won't know what you got til you grow them out!~


wanna go fishing RR?
:grin:



tc


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!

Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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