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OfflineRogerRabbitM
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Re: cloning and strain isolation [Re: MYSTIQUE]
    #7913937 - 01/22/08 09:30 AM (16 years, 29 days ago)

Quote:

MYSTIQUE said:
You have your strain isolated in a slant or dish. You should have a few different strains isolated from the original.

You take a small piece and put it in grain or pf and then you let it fruit.

You see if it has the quality's that you like.

You should have a large slant or dish that you labeled and put in the fridge that goes with your fav jar.

That is now your master culture. Take a small part of it and save the rest.

Take the small part and put it in a new slant or dish and use that to make spawn and what not.




:thumbup:  Couldn't have said it better.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleWolfgang
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Re: cloning and strain isolation [Re: RogerRabbit]
    #7913965 - 01/22/08 09:42 AM (16 years, 29 days ago)

I guess my understanding is still pretty flawed then I could have swore I read that potency varies from mushroom to mushroom on any given flush.. including flushes from isolated strains

but I see that can't be true.. thanks


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OfflineMYSTIQUE
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Re: cloning and strain isolation [Re: Wolfgang]
    #7914007 - 01/22/08 10:02 AM (16 years, 29 days ago)

Well potency isn't totally controlled by genetics. Get the best isolate ever and grow it on white rice flour and it will suck.

And thanks RR I learned everything about cloning mushrooms from you.


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Dont know what the fuck I just said? READ THIS
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I ain't a hippy but I'm covered in dirt                         
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Chasin' the Grateful Dead, no shoes on my feet
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:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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InvisibleWolfgang
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Re: cloning and strain isolation [Re: MYSTIQUE]
    #7914397 - 01/22/08 11:58 AM (16 years, 29 days ago)

It's nearly exclusively controlled by genetics according to RR he's grown on everything with no difference in potency between in substrates.


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OfflineMYSTIQUE
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Re: cloning and strain isolation [Re: Wolfgang]
    #7914415 - 01/22/08 12:05 PM (16 years, 29 days ago)

naw you cant use sub to make super strong shrooms but you can use it to make weak ones. Just look up any of the white rice flour grows.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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InvisibleWolfgang
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Re: cloning and strain isolation [Re: MYSTIQUE]
    #7914449 - 01/22/08 12:19 PM (16 years, 29 days ago)

Quote:

MYSTIQUE said:
You are cloning when you cut the section out of the slant and droping it into the jar in a really loose sense of the word.




What's the traditional direct mushroom clone used for then? Wouldn't this definition of a clone differ greatly from that of a cloned mushroom?

When we cut the section out of the slant and drop it in the jar to test for the qualities we like, that flush will have many mushrooms of DIFFERENT potencies correct but if we cloned a specific mushroom that flush would not have different potencies from mushroom to mushroom?


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OfflineRogerRabbitM
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Re: cloning and strain isolation [Re: Wolfgang]
    #7914680 - 01/22/08 01:24 PM (16 years, 29 days ago)

When you transfer from a slant or petri dish, you're not cloning. Cloning is taking live tissue from a fruit. Transferring from a slant or petri is simply transferring.

About the white rice thing. It is a crappy substrate but doesn't produce non-active mushrooms. Many new growers try different things, and thus when they get a bum batch, they often think it was the substrate. Ditto for when they get a killer potency flush. Over the years, I've heard dozens of claims of inactive mushrooms, and extremely potent mushrooms over just about every strain and every known substrate.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleWolfgang
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Re: cloning and strain isolation [Re: RogerRabbit]
    #7917024 - 01/22/08 08:40 PM (16 years, 28 days ago)

So when do we clone!?


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Invisiblesimplemachine
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Re: cloning and strain isolation [Re: RogerRabbit]
    #7917816 - 01/22/08 10:40 PM (16 years, 28 days ago)

Quote:

So when do we clone!?




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Offlinelaten
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Re: cloning and strain isolation [Re: simplemachine]
    #7917864 - 01/22/08 10:51 PM (16 years, 28 days ago)

You clone from the upper part of the mushroom stem when its done fruiting. Or you could put a pin on a plate and clone that.


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Invisiblethedefone
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Registered: 10/06/07
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Re: cloning and strain isolation [Re: simplemachine] * 1
    #7917948 - 01/22/08 11:08 PM (16 years, 28 days ago)

Here is a good link on sectoring. I'm posting this for my benefit, and for the other idiots like me, who didn't quite know. Also, thanks simplemachine for that link.

My question. At what point does senescence start to kick in? If it takes multiple sectorings to finally establish the ideal strain, how close are you to genetic breakdown at that point? Or am I totally missing it?


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I am become death, the destroyer of worlds.


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OfflineRogerRabbitM
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Re: cloning and strain isolation [Re: thedefone]
    #7918014 - 01/22/08 11:20 PM (16 years, 28 days ago)

Quote:

how close are you to genetic breakdown at that point?




You're still a long way off from senescence. Mycelium will generally grow aggressively for several months. Your isolations will be done within a month to six weeks. At that point, save all your strains in the refrigerator to stop growth(more accurately-slow the growth way down). When the cells stop dividing, the road to senescence gets much longer. This means that two years from now, you can pull a master slant out of the refrigerator and it will realistically only have that original six weeks worth of cell divisions on it.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisiblethedefone
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Re: cloning and strain isolation [Re: RogerRabbit]
    #7918082 - 01/22/08 11:34 PM (16 years, 28 days ago)

Okay. So, that will only start being an issue if you are taking clones from, say, isolated fourth-flush fruit bodies.

Also, when you take sectors and transfer them to new plates, they do continue to re-sector themselves, no? But, the genetic variation within those resulting sectors is narrower, yes? Just thinking out loud...


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I am become death, the destroyer of worlds.


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InvisibleWolfgang
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Re: cloning and strain isolation [Re: RogerRabbit]
    #7918825 - 01/23/08 05:56 AM (16 years, 28 days ago)

Would a proper agar isolate be considered more "pure" in a genetic sense than a culture from a cloned tissue sample that was taken from an isolated line of mycelium?

Would a cloned tissue sample taken from an isolated line of mycelium PERFORM like it's mycelium would?

How can a cultivator have consistently great results in terms of potency AND performance?


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Invisiblethedefone
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Re: cloning and strain isolation [Re: Wolfgang]
    #7926804 - 01/24/08 09:21 PM (16 years, 26 days ago)

bump.


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I am become death, the destroyer of worlds.


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Offlinecyberthreat
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Re: cloning and strain isolation [Re: thedefone]
    #8016395 - 02/13/08 06:22 AM (16 years, 7 days ago)

yeah i was getting bad potency on straw pf tek then i got some poo
from a sponsor grew out albinos and before i was eating maybe 300 grams of wet from my pf cake days and straw so ate 3 gram of the albino and i couldn't walk and the visuals were amazing life changing


Edited by cyberthreat (02/13/08 11:04 AM)


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