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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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making a liquid culture with a tiny piece of mycelium
    #5745128 -

So I know you can clone shrooms by taking a piece of sterile tissue from the inside of the stem and then grow that out on agar....

But, is it possible to make an LC (just the simple honey water tek) by snipping off a tiny piece of mycelium from the surface of a casing and dropping it in the water? I ask this cause I have one casing of PESA going right now, and Im liking its growth characterisitcs alot more than the cambos I'm doing as well. Not for colonization times of the jars or anything but cause of how the mycelium comes through the casing seemingly with alot more ease. Might have something to do with the fact that its so damn rhizomorphic.

Anyway....that PESA syringe is empty now and I dont wanna wait for the shrooms to have grown before continuing the cycle. My Cambo mono-tub didnt really work as flawlessly as I had hoped, nor did the mini bulk casing of cambo...I basically wanna start another round of PESA jars to re-do the monotub with that strain as soon as possible. So, now that I've told you guys my life story.....is it possible to snip of a tiny piece of the mycelium which is coming through to the surface and grow it out in a liquid culture?

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Re: making a liquid culture with a tiny piece of mycelium [Re: iamyour_messiah]
    #5745178 -

I'm not the shroom king or anything, but i think the answer is yea that should work. Mycelium can be eaten just like a shroom. So i don't see why it wouldn't continue to spread when it hits a none colonized substate (the LC) think of when you case and the mycelium takes over most of the caseing..

I'm still a noob, but i read alot!! So i may be wrong, and i'm sure you'll get some replys from some other very knowledgeable ppl soon.


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Going to miss the PNW gatherings.

I'm not here trying to make anyone feel stupid or to make myself feel good by putting ppl down. I am just here to help fellow shroomerites in there grows. I do this to keep my own knowledge fresh, and to help everyone succeed!

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Re: making a liquid culture with a tiny piece of mycelium [Re: SickShroomer]
    #5745196 -

I see no reason why it would not work, just take alot of precaution to sterilization. I hope it all goes well for you, meaning no contams. GL


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.




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Re: making a liquid culture with a tiny piece of mycelium [Re: iamyour_messiah]
    #5745212 -

iamyour_messiah said:
is it possible to snip of a tiny piece of the mycelium which is coming through to the surface and grow it out in a liquid culture?


better learn about agar, the fact that it's been removed from the jar has opened it up to airborne contaminants, the only way to clean it up is to run it out on agar.


SickShroomer said:
Mycelium can be eaten just like a shroom.


I dont recomend it, besides everyone here calling you glitter shits, you wont get much if anything from it.


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California Has Legalized Child Prostitution

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Re: making a liquid culture with a tiny piece of mycelium [Re: Prisoner#1]
    #5745262 -

k well Ill have to wait till I actually have the shrooms in that case....should only delay my plans by like 1 to 2 weeks max.

Is it possible to take some sterile tissue from near the base of a freshly picked shrooms and drop that in the LC, or will this only grow on agar?

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Re: making a liquid culture with a tiny piece of mycelium [Re: Prisoner#1]
    #5745472 -

Prisoner#1 said:
iamyour_messiah said:
is it possible to snip of a tiny piece of the mycelium which is coming through to the surface and grow it out in a liquid culture?


better learn about agar, the fact that it's been removed from the jar has opened it up to airborne contaminants, the only way to clean it up is to run it out on agar.


SickShroomer said:
Mycelium can be eaten just like a shroom. 


I dont recomend it, besides everyone here calling you glitter shits, you wont get much if anything from it.


Peroxided LC will work fine for tissue transfers but as far as eating myc well glitter shits :wink: I highly do not recommend wasting your time on it. Much respects


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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