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Dal33tdude12
Psychotic Killa

Registered: 05/22/06
Posts: 52
Last seen: 17 years, 2 months
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Contams!!!
#5738194 - 06/11/06 02:35 PM (17 years, 7 months ago) |
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Ok so ive been checkin on my jars every day or every other day to see how they are doing i have pulled 3 jars so far that are contaminated. I took some from The Magic Mushrooms Growers Guide and some from Shroom Wizard s Grow Guide. So what i did was made my substrate BRF wit Verm. mixed put in jars, and put the dry layer of verm. (and yes i prewashed the jars) on top of the substrate about 3/4 of an inch, but on half of the jars i forgot to wipe the sides of the glass down before putting the dry layer of verm down. so im thinking thats why im getting contams. I didnt realize i had done that until i was half done with everything. I then put foil over the lids and boiled them for about a hour. i have no PC so i couldn't PC them. Took them out let them sit overnight took foil off then Innoc. the 4 holes i put in the lids. Put them in my incubator and again like i said been checking every day or every other day. Any help would be appreciated!
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whatever123
Whatever I did, I'm sorry


Registered: 04/07/05
Posts: 2,613
Loc: San Diego, CA
Last seen: 5 years, 6 months
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Best advice I can give you: buy a PC.
-------------------- Koala Koolio said: there should be a 3 month waiting period between registration and posting.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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tell me what kind of contams. tell me where in the jar the contam was.
boil your jars for more like 2 hours, not one. what is your inoculation technique?
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austin_72283
Mr. or Sir


Registered: 10/16/03
Posts: 157
Loc: west
Last seen: 17 years, 5 months
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i tried with boiling first and its just not worth the fustration of seeing contams. i have a pc and have not gotten any contams. ( knock on wood)
also use a glove box to inoculate you can make one for under 5$
and just be as clean and sterile as possible i.e Lysol is your friend and you should spray it everywhere. peroxide and alcohol is good to have too to whipe down everyting with.
flame your needle after each inoculation.
Austin
-------------------- a job worth doing is a job worth doing right.
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gutmaggot
Stranger
Registered: 04/21/06
Posts: 107
Last seen: 16 years, 10 months
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i knocked up 35 jars june 2nd and all of them have signs of growth with no contams. about 25% of them look like they will be done colonizing on the outside within 2-3 days. no sign of contams. i used agars open air tek. just brf jars pf tek boiled for an hour and 15 min and let cool overnight.
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Dal33tdude12
Psychotic Killa

Registered: 05/22/06
Posts: 52
Last seen: 17 years, 2 months
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I got green contams in one jar, in 2 others it has a yellow contam with little spore looking things growing out of it it was all towards the top of the jar. Thats why im pretty sure it was cause i didnt wipe the sides down. i went into my bathroom and sprayed the air with bleach and lysol waited about 10 minutes, flame sterilized the needle after every 3 jars. i will haft to make a glove box or open air box.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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neither lysol or bleach will clean the air.
use oust for air.
use a wipe of vinegar followed by a wipe of isopropyl for surfaces.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Actually, a ten percent bleach solution sprayed in a fine mist into the air is one of the best ways to sanitize the air a room. Easily as good as oust and much cheaper. I measured a 99% drop in airborn particulates after a bleach bomb. Oust isn't as effective, since it only attacks bacteria spores. Bleach attacks bacteria as well as mold spores, then carries them harmlessly to the floor as the mist settles. The problem is bleach ruins your clothes, carpet, bedding, nose and eyes. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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