trentallica said:

i don't have an pressure cooked, but i do have an autoclave and have been using it.
an autoclave dose not have a max pressure or temp setting. it just gets as hot as it going to get, and gets as much pressure as its going to get. it is used to sterilize med equipment so there in no need for max limits. when i use it pressure gets as high as 30 psi, and temp over 260 F.
everything i have inoculated past a certain point shown no sign of growth. ok to be fair some of it was inoculated today, but still. I'm trying to think what i did differently the first two times that I'm not doing now. one thing that comes to mind is the autoclave. my first two attempts both came from the same autoclave cycle. the cycles that i have used after that have all been on longer cycles just to "be on the safe side", so they have all had longer exposure to ever higher temps , and higher psi then the first cycle.
i know nutrients are not alive, but i know proteins ans amino acids are involved. could the autoclave be converting the nutrients to an unusable state??????

---

BooNanZa said:

I too was wondering this. I have access to an autoclave at work, and it has two settings, LIQUID (for liquid cultures and garbage bags) and GRAVITY (for beakers, etc.) and wanted to know if I could throw some spawn bags in there since they won't fit my PC. It also goes to about 30psi, with a run of 35 minutes (20 minutes sterilize, 15 minutes dry)

---