Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Vermiculite

Jump to first unread post. Pages: 1
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Liquid Growth Medium
    #4216595 - 05/25/05 12:25 AM (11 years, 6 months ago)

ok, my question is about Liquid Growth Medium.
i want to make some. but i cannot find malt anywhere.
i have plenty of dextrose and cornmeal. this can be substituted into agar mix for mal does anyone have experience with other liquid recipies? i have some mycelium going on soaked cardboard for eventual agar or whatever.
but liquid would be better.
any other ideas would help alot too the FAQ only has one recipie.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineliveby
Wasted For Time
 User Gallery

Registered: 06/15/04
Posts: 1,511
Last seen: 5 years, 7 months
Re: Liquid Growth Medium [Re: Mitchnast]
    #4216614 - 05/25/05 12:30 AM (11 years, 6 months ago)

i use sugar cane syrup , works a charm


--------------------

http://www.bruceeisner.com/ -Creating a Sensible Culture


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleQuiescence
Intriguer
 Arcade Champion: Hexxagon

Registered: 04/25/05
Posts: 315
Re: Liquid Growth Medium [Re: Mitchnast]
    #4216618 - 05/25/05 12:31 AM (11 years, 6 months ago)

Try using a 5% karo solution. It is great because it wont camerlize when you PC it. Also, it's clear so you can see any growth that is happening.

GOod luck


--------------------
God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,418
Loc: Nowhere
Re: Liquid Growth Medium [Re: Mitchnast]
    #4216647 - 05/25/05 12:37 AM (11 years, 6 months ago)



--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
Hunter S. Thompson

It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineOatman2000
-=Outa Space=-
Male User Gallery

Folding@home Statistics
Registered: 05/10/05
Posts: 2,863
Loc: Planetary Nebula
Last seen: 22 days, 2 hours
Trusted Cultivator
Re: Liquid Growth Medium [Re: MrMaddHatter]
    #4216733 - 05/25/05 01:13 AM (11 years, 6 months ago)

Honey works good too, just take a 1/2 pt canning jar and add 1 tsp of honey into "hot" water. then inoculate this jar just like you would a pf jar. Dextrose is better



--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Liquid Growth Medium [Re: Mitchnast]
    #4216855 - 05/25/05 01:50 AM (11 years, 6 months ago)

10ml.dextrose/100ml. h2o works.
you can add a pinch of brewers yeast 4 an extra kick, the LC will look like beer.
6ml.dextrose/6ml.potato flakes/100ml.h2o even better.
its a little cloudy, but contams will show up if present.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,898
Last seen: 1 year, 28 days
Re: Liquid Growth Medium [Re: deanofmean]
    #4216902 - 05/25/05 02:03 AM (11 years, 6 months ago)

Dextrose is my fav.

2nd best would have to be vanilla karo :grin:

-Gnostic


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineIceCube
Stranger
Registered: 09/18/04
Posts: 60
Last seen: 7 years, 3 months
Re: Liquid Growth Medium [Re: IGnosticAbhorI]
    #4216986 - 05/25/05 02:23 AM (11 years, 6 months ago)

I use Karo tek, works awesome for me.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Re: Liquid Growth Medium [Re: IGnosticAbhorI]
    #4217011 - 05/25/05 02:28 AM (11 years, 6 months ago)

ok i like the dextrose idea.
now about established mycelium.
i want to add some already growing white matter.

i was thinking a dip in h2o2 then into the liquid in the glovebox.
would it stay a lump? or would it spread throughout?
i read somewhere that adding broken glass for stirring will help.

now i know liquid media is better at outrunning contams if you arent as careful. right now i get about a third success with multispore direct to grain. half the failures contam and the other half don't germinate i figure this will cut that alot.

as for contamination in liquid, is this really common?
will established myc outcompete this any?
oppinions of course, im casting absolute questions, but im fishing for brain food.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Re: Liquid Growth Medium [Re: Mitchnast]
    #4217082 - 05/25/05 03:10 AM (11 years, 6 months ago)

alrighty, so im going to do a test.
ive got 4 4oz baby formula bottles.
nipple tops with tyvek. (yes i have a baby, she has a heart condition so i also get alot of free syringes, test tubes, caps, etc ive got a whole crate of 60cc syringes.)
anywho i used 75ml water, one level tsp corn dextrose, and about 5-6 little balls of slow yeast.
its all in the PC now. its tap water but i figure by tomorrow after sitting in the glovebox they should be pretty much dechlorinated.

since im starting with growing myc it probably wont be too harsh anyway, since theres no spores involved.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,898
Last seen: 1 year, 28 days
Re: Liquid Growth Medium [Re: Mitchnast]
    #4217168 - 05/25/05 03:38 AM (11 years, 6 months ago)

LC's the way to go...

But if you're not sterile....you'll fuck yourself over - Badly

-Gnostic


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Re: Liquid Growth Medium [Re: IGnosticAbhorI]
    #4217213 - 05/25/05 03:58 AM (11 years, 6 months ago)

well yeah. im being as careful with these as when i make syringes, which lately i havent done. when i do agar, on average, 1 in 5 contaminate of course round the edges. the stuff i let up on is bulk, and never had a problem once im there.
i just dont want to be messing around with other peoples syringes anymore. i want to know if i get contams, its my fault. if one of these 4 test jars works the first time, then ill do larger batches off of those.
i can see thogh that these are more suseptable to contamination as they really dont seem to be the type to become fully colonized.
i plan to store my tiny jars in a tub, and put that within an incubator, the tub with have a polyfill vent and i will occasionally give the interior an alcohol spritz.
i dont really work in a sterile environment, but i employ a glovebox for the sensitive work


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Liquid Growth Medium [Re: Mitchnast]
    #4217221 - 05/25/05 04:01 AM (11 years, 6 months ago)

from what it said in UR first post, U started UR spores on cardboard, wright?
this puts you way out in front of any bacteria or mold spores.(thy won't germinate on cardboard).
add 1 ml of 3% h2o2 per 100ml to the LC then drop UR cardboard in,  contams won't stand a chance. :grin:
this is the exact tek i use for starting wild prints, and hasn't failed me yet.
:thumbup:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Re: Liquid Growth Medium [Re: deanofmean]
    #4217255 - 05/25/05 04:14 AM (11 years, 6 months ago)

no i started spores on grain when i put it to the straw i tossed a few grains into a open ziplock bag of soaked cardboard, just to get some myc going outside of active projects for later isolation.
i figured if i peel some hunks from the cardboard, h2o2 them, and drop them in the LC that would work.
but basically there is grain present, it is however, fully colonized.
are you aying you've actually GERMINATED on cardboard?
im going to try it later with tissue from fruiting bodies once i have some. prolly in a week the way things are going


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan Flag
Last seen: 28 days, 6 hours
Re: Liquid Growth Medium [Re: Mitchnast]
    #4217265 - 05/25/05 04:20 AM (11 years, 6 months ago)

ooooh, i just checked, and theres primordia starting :smile:
this is gonna be great, this is the first time i got the casing even with no overlay.
i used to fruit late evry time.  now im seing primordia popping out from under verm. joy.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Liquid Growth Medium [Re: Mitchnast]
    #4219551 - 05/25/05 07:53 PM (11 years, 6 months ago)

Quote:

Mitchnast said:
no i started spores on grain when i put it to the straw i tossed a few grains into a open ziplock bag of soaked cardboard, just to get some myc going outside of active projects for later isolation.
i figured if i peel some hunks from the cardboard, h2o2 them, and drop them in the LC that would work.
but basically there is grain present, it is however, fully colonized.
are you aying you've actually GERMINATED on cardboard?
im going to try it later with tissue from fruiting bodies once i have some. prolly in a week the way things are going




yes, cardboard works grate for germinating spores.
as i said, bacteria and mold spores don't seem to like it.
the gray cardboard like from the back of a legal pad works best.
i use a hole punch to make little disks, soak them 24 hours to get max water content,then PC (or steam, nuke, whatever) in a baby food jar.
now comes the fun part, using tweezers touch the edge of the disk to a print, put it back in the jar, and throw it in your incubator.
in about 2or3 days the spores should have sprouted, the myc. will look like powdered sugar.
its ready to place on peroxided agar, or in LC w/ 1ml h2o2 per 100ml.
watch it grow :smile:
i have been doing this for 3 years now w/ vary few contams, even when done without a glove box.


Edited by deanofmean (05/26/05 07:59 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Elementary question regarding Liquid Growth Medium Stix Russell 695 3 02/27/04 01:45 PM
by MilkVein
* Q about Liquid Growth Medium Tek kid_alamo 732 2 08/09/02 01:45 PM
by kid_alamo
* Q about Liquid Growth Medium Tek kid_alamo 806 3 08/10/02 09:41 AM
by kid_alamo
* liquid growth medium 1uvu 789 7 02/17/05 04:16 PM
by midway
* Liquid Growth Medium.. acadian 582 5 12/14/05 10:06 PM
by Rahz
* Liquid Growth Medium Ainasko 568 4 05/15/03 02:27 PM
by Ainasko
* Agar Liquid Growth Medium Question Anonymous 673 3 03/20/03 04:28 PM
by Baby_Hitler
* liquid growth medium weedpreference 494 2 01/02/06 11:00 PM
by weedpreference

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,422 topic views. 25 members, 83 guests and 31 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.057 seconds spending 0.001 seconds on 14 queries.