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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Reagent Testing Kit

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InvisibleAinasko
Oksania
Registered: 04/26/03
Posts: 694
Liquid Growth Medium
    #1550965 - 05/15/03 02:10 PM (14 years, 11 months ago)

I've used the Liquid Growth Medium tek to innoculate many jars from a single spore syringe.

I thought I had another prepared syringe in my refrigerator, but I was mistaken and was wanting to innoculate a few more jars this weekend.

I can't think of any reason why I can't just add established mycelium to my powdered malt extract solution. Has anyone tried this with success and if so, could you please offer a few tips?

Thanks, in advance.


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Ainasko is my name backwards. I'm a girl!


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Invisibleafoaf
CEO DBK?
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Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
Re: Liquid Growth Medium [Re: Ainasko]
    #1550980 - 05/15/03 02:14 PM (14 years, 11 months ago)

even better, using existing liquid cultures
to innoc subsequent batches.

less time waiting for spores to germinate.

but it is worth noting that doing that is similar
to grain to grain transfers, in that any latent
contamination (invisible or otherwise) will
be transfered from the old batch into the fresh
new batch.

in short, make sure the source is clean.

h202 is your friend.


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All I know is The Growery is a place where losers who get banned here go.


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Offlinefugu
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Registered: 01/06/03
Posts: 2,223
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Re: Liquid Growth Medium [Re: afoaf]
    #1550994 - 05/15/03 02:19 PM (14 years, 11 months ago)

agars are faster then g2g
you win 2 days at least


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mushroom culture history making ...Mr. Allan is the best .....


Edited by fugu (05/15/03 02:20 PM)


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
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Re: Liquid Growth Medium [Re: afoaf]
    #1551003 - 05/15/03 02:21 PM (14 years, 11 months ago)

Just watch out -- if you do it too many times the strain will degrade (esp. if using the same media each time.) S'why petries are better -- you can actually look at the mycelium and sector rhizomorphic parts which will prolong the strain's good characteristics instead of taking random parts.

--
Micro


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InvisibleAinasko
Oksania
Registered: 04/26/03
Posts: 694
Re: Liquid Growth Medium [Re: afoaf]
    #1551039 - 05/15/03 02:27 PM (14 years, 11 months ago)

Thanks so much, afoaf .

I periodically use a Sterilization Test Kit, but only when I work with bulk teks. I know it's not 100% effective, but it gives me a little more confidence against contaminants.

If you want one just look up Sterilization Test Kits on any search engine. A friend of mine owns a body piercing studio and told me about them.

They are usually about $5 a test and are well worth the alternate aggravation of lost time and effort.

I've also been using bacteria water test kits. They are meant for people with low immune systems to test water for harmful bacterias, but are great for testing moist substrates as well. A cheap bacteria test kit can be found online or at larger pharmacies for around $20.

Thanks again for the info, afoaf. See you around, pal.


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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Reagent Testing Kit

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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