Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MushroomMan Mycology
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinetoo_many_weirdos
it's a jungle inhere
Registered: 12/01/01
Posts: 517
Loc: Ithaca, NY
Last seen: 19 years, 1 hour
Im going to grow sclerotia invitro. ideas?
    #549346 - 02/12/02 07:58 PM (21 years, 9 months ago)

Ok, so i've decided to investigate the virtues of Ps. mexicana and invitro sclerotia formation. These are my plans:

5 one-pint jars.
2 CC spore solution (A-strain) per jar. (should i use more juice?)
Substrate will be: 1 part rye grass seed to 3 parts water.
pressure cooked for 30-45 mins.
Micron filter discs on the jars to allow for air circulation.
After colonization and trashing of contaminated jars, they will be placed in a dark cabinet and allowed to chill there for a couple months, blowing some air into the cabinet every once in a while to insure fresh air.
when it seems that sclerotia is done forming, i will harvest the jars.

does this seem good to everyone? are there any suggestions you people have for me and my experiment?


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,593
Loc: Oregon, USA
Last seen: 8 hours, 59 minutes
Trusted Cultivator
Re: Im going to grow sclerotia invitro. ideas? [Re: too_many_weirdos]
    #549458 - 02/12/02 09:57 PM (21 years, 9 months ago)

Use larger jars if possible, leave 1/4 or more of the jar as empty air space (keep in mind that the seed swells when hydrated during pressure cooking).
Ratio of seed to water is 2:1 not 1:3 (unless you are doing a steeping and draining method)
Pressure cook for a full hour at 15 PSI
Filter disk is fine but these jars will be incubating a long time and will dry out if the hole the filter disk is covering is too large. Try a 1 centimeter hole or smaller and cap the top of the jar with foil.
Blowing air in isn't helpful

Other than that your method is perfect.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Extras: Filter Print Post Top
Offlinetoo_many_weirdos
it's a jungle inhere
Registered: 12/01/01
Posts: 517
Loc: Ithaca, NY
Last seen: 19 years, 1 hour
Re: Im going to grow sclerotia invitro. ideas? [Re: Workman]
    #550071 - 02/13/02 01:01 PM (21 years, 9 months ago)

dandy.

^bump^


Extras: Filter Print Post Top
Offlinetoo_many_weirdos
it's a jungle inhere
Registered: 12/01/01
Posts: 517
Loc: Ithaca, NY
Last seen: 19 years, 1 hour
Re: Im going to grow sclerotia invitro. ideas? [Re: too_many_weirdos]
    #551420 - 02/14/02 02:59 PM (21 years, 9 months ago)

You guys are useless.
well except for workman that is.

i need more input.


Edited by too_many_weirdos (02/14/02 03:13 PM)

Extras: Filter Print Post Top
Anonymous

Re: Im going to grow sclerotia invitro. ideas? [Re: too_many_weirdos]
    #551433 - 02/14/02 03:08 PM (21 years, 9 months ago)

You just got all the input you needed.


Extras: Filter Print Post Top
Offlinetoo_many_weirdos
it's a jungle inhere
Registered: 12/01/01
Posts: 517
Loc: Ithaca, NY
Last seen: 19 years, 1 hour
Re: Im going to grow sclerotia invitro. ideas? [Re: ]
    #551450 - 02/14/02 03:19 PM (21 years, 9 months ago)

well, fine


Extras: Filter Print Post Top
Anonymous

Re: Im going to grow sclerotia invitro. ideas? [Re: too_many_weirdos]
    #551557 - 02/14/02 04:24 PM (21 years, 9 months ago)

Innoculate sterilized ryeseed. Wait three months and harvest. That is the beauty of invitro sclerotia production. Wasn't trying to be a dick.

Larger jars are better, larger yields per jar. Filters are not absolutely neccessary, but alittle clean gas exchange can't hurt.

If you really want to get crazy, Use two of your healthiest looking pint jars to innoculate 14 more pint jars.

Sclerotia are best consumed fresh.

Expect yields of 15-30 dry grams per cup of dry ryeseed. Very High Yielding.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Autoflowering Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* anyone have success growing creeper invitro? disturbed 947 3 12/08/02 12:56 PM
by disturbed
* Re: Making truffles Taz 4,474 18 04/13/01 11:05 AM
by tom
* Re: Sclerotia Formation CLuB99 6,556 17 03/31/00 12:13 PM
by Workman
* Problem Growing Invitro DaGeniusE 1,166 3 07/07/02 03:50 AM
by CHeeCHer
* Creepers grown invitro style? volcomYAE23 1,127 8 11/22/02 09:28 PM
by TeRzMaStA
* Invitro - filter discs? matamore 662 1 08/08/01 11:44 AM
by Shiznitz
* Narly invitro boomer - safe? (pics) ed: more pics Manzarek 997 6 12/15/02 02:55 PM
by BC-Shroom
* whats a small shroom to grow? Anonymous 733 4 12/01/02 11:46 PM
by psilocybinjunkie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,315 topic views. 14 members, 136 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2023 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 12 queries.