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Offlinenewbiegrower
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Registered: 12/13/01
Posts: 10
Last seen: 22 years, 1 month
Rye Contamination Hypothesis
    #521936 - 01/16/02 01:38 AM (22 years, 1 month ago)

From my understanding from reading here and on my own many peole have problems with rye grain because of contaminants that lie dormant inside the rye grain itself. Furthermore, I believe that active bacteria are killed at much lower temperatures than are dormant bacterial spores. Thus, might it be possible to eliminate this type of contamination by actually encouraging germination of those spores that are normally dormant within the rye grain. Unfortunately I don't know enough about these bacteria to know what triggers their awakening. I think it could be many things from light to water or even high temperatures. Maybe a good starting place would be to let the rye grain just sit on the counter for a few days while soaking in water. I know that teks already suggest soaking the rye overnight but I think that the stated reason is to acheive proper moisture content not eliminate contams. Any thoughts or comments would be welcome before my friend tries this


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OfflineElektrolurch
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Re: Rye Contamination Hypothesis [Re: newbiegrower]
    #521954 - 01/16/02 01:58 AM (22 years, 1 month ago)

As far as I know the "24 hour soaking Method" is to achieve the germination of the endospores. Germinated endospores (=bacteria) are much more heat intolerant. Therefore it is for the reduction of contaminations.

There is actually a method for the sterilization of media based on the fact that bacteria are much more intolerant to heat than endospores. It is called tyndilization or fractional sterilization. It consists of pasteurizing, incubating 24 hours, pasteurizing, incubating, etc... This is made of up to 5 times.

Moisture is the trigger of germination. Endospore production is encouraged by high temperatures in a dry atmosphere.

Elektrolurch


--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67


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OfflineAnnoA
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Re: Rye Contamination Hypothesis [Re: newbiegrower]
    #521982 - 01/16/02 02:30 AM (22 years, 1 month ago)

If you soak the rye any longer than 24 hours it will germinate, sometimes even before that.


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Offlinenewbiegrower
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Registered: 12/13/01
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Last seen: 22 years, 1 month
Re: Rye Contamination Hypothesis [Re: Anno]
    #524115 - 01/18/02 01:33 AM (22 years, 1 month ago)

is it the endospore or the endosperm that is supposed to germinate. the endosperm is the food for the developping seedling (rye seedling) while the endospore is the dormant bacteria that is unwanted. I know that soaking in water for 24 hours will germinate the endosperm and begin maturation of the seed. this undoubtedly makes the environment more suitable for mycoculture because it causes complex starched to begin to break down into sugars which are more easily metabolized. However I am still not convinced that the endospres are explicitly involved


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Anonymous

Re: Rye Contamination Hypothesis [Re: newbiegrower]
    #524123 - 01/18/02 01:44 AM (22 years, 1 month ago)

You soak the rye grain for no more then 24 hours, as stated. This allows the bacterial endospores to germinate, and allows the rye to absorb moisture. It is now easier to kill all the contaminants present, and you have uniform moisture content.
Once the rye starts to germinate or even open up, everything gets way to sticky to make it a good spawn substrate.
The object is to kill contaminants, not improve on the nutritional quality of the rye!!!
The other recommendation works on the same principal, except you can do the killing without a pressure cooker. It just takes more time.


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