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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Is there a pasteurisable substrate suitable for sclerotia?
    #4892694 -

I am thinking maybe straw? People have mentioned straw pellets before, what are they used for? i.e. what sort of place would stock them?

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Re: Is there a pasteurisable substrate suitable for sclerotia? [Re: blackout]
    #4893219 -

I can't add much about straw or straw pellets, but have you considered peroxide treatment of rice?

I'm having pretty good luck after two weeks with brown rice that I added h2o2 to and then inoculated with L.C. of turkeytail. I had autoclaved the rice, but it became a solid lump and so I turned each pint out into a mixing bowl and broke it up by hand adding first the peroxide and then the L.C. Definitely a non-sterile treatment.

What I don't know is how much heat is required to deactivate the peroxidase. Would simple cooking do it?

I've seen peroxide treament of straw work well. 1% overnight followed by three rinses and spawning with Pleurotis on rye.

Rush Wayne ('Growing Mushrooms the Easy Way', Volume II)recomends 0.15% peroxide plus 10 mls/liter vinegar, covering straw completely. Four hours if straw is chopped, 24+ hours if straw is whole. Don't rinse.

Q: Don't pasteurized substrates tend to contaminate over time? And isn't mexicana slow to colonize? Or is it just slower to mature the sclerotia?

Best of luck! I'll be watching for reports.
Tsin

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Re: Is there a pasteurisable substrate suitable for sclerotia? [Re: Tsinaglou]
    #4893288 -

Sclerotia will form great on rye berries. Whatever you spawn your spores to is what will grow awesome sclerotia. Just hydrate it with weak coffee, inoculate and wait three months.
RR


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Re: Is there a pasteurisable substrate suitable for sclerotia? [Re: Tsinaglou]
    #4912493 -

Tsinaglou said:
Don't pasteurized substrates tend to contaminate over time? And isn't mexicana slow to colonize?


Yes, but all subs that are under normal mushroom fruiting instances will contam. I have heard that the tenn stud prewetted pastuerised subs stay fine in the bag for months. I would be injecting a lot of LC to colonise quickly, hopefully the mycs natural defences would stop contams.

Roger- have you ever heard of sclerotia growing on straw? It is mentioned in TMC as a sub for fruiting so I guess somebody must have fruited mex or tamp on straw and may have got sclerotia in it too. I am not sure if it should get the 70% water content. My sclerotia seems to be doing better on the drier RGS.

EDIT just found this thread where EonTan says it works well on straw
http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=3951428

and found this thread (that I even posted in and cannot remember at all!) a guy specifically growing sclerotia on cold lime pastuerised straw.
http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Growlog&Number=1790932

does anybody know what weight of hydrated lime (55% neutralising) should be added per litre of water to give me a ph of 11-12?

Edited by blackout (11/09/05 06:50 AM)

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Re: Is there a pasteurisable substrate suitable for sclerotia? [Re: blackout]
    #4914505 -

I had success going from multispore or clone to Ryegrass seed(sterilized) to Pastuerized straw.

The sclerotia are a pain in the ass to seperate from the straw, but they got huge.

Fruits were nice also.

I just inoculated the straw in large turkey pans, and when colonized, cased with peat:vermiculite and it began to fruit two weeks later. Lots of sclerotia fromed in the casing layer, and below in the straw.

I never did a lime bath. I have used Peroxide with success on Mixed compost(wood chip/farm manure) with weilii. Never tried it with straw yet.

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Re: Is there a pasteurisable substrate suitable for sclerotia? [Re: EonTan]
    #4916681 -

Sounds good, the thing I hate about straw is the low density. I was thinking of finely shredding straw and then adding maybe BRF to it to add some weight but still be airy. Should be sterile with a 90minute boil.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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