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OfflineFood
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Can Mycelium die of coldness ?
    #486019 - 12/10/01 03:19 PM (15 years, 3 months ago)

I am asking because I foolishly let some culture jars out of their warm incubation unit and left them in a cold place at around 5-8 degrees celcius overnight and since then even though I have put them back into the chamber they seem to have just stopped spreading .

Are they just shocked d'you think or are they toast ?

TIA

:)


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Edited by Food (12/10/01 06:23 PM)


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OfflineMAIA
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Re: Can Mycelium die of coldness ? [Re: Food]
    #486034 - 12/10/01 03:27 PM (15 years, 3 months ago)

It only dies at freezing point i think. Wait a couple of days and see.

Peace,
MAIA


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Anonymous

Re: Can Mycelium die of coldness ? [Re: Food]
    #486096 - 12/10/01 04:26 PM (15 years, 3 months ago)

Get your temps up and the mycellium should revive. In nature, muchroom mycellium is subjected to freezing every winter but always comes alive in the spring when warmer weather returns.
cubensis mycellium may be a little different because it comes from warmer countries but the spores have proven to still be viable after freezing temps, I bet the mycellium does too.


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OfflineFood
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Re: Can Mycelium die of coldness ? [Re: ]
    #486106 - 12/10/01 04:34 PM (15 years, 3 months ago)

Cheers dudes I feel better now but I still dont know for sure whether they'll be ok - does anyone know for certain how low the temperature can go ?


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OfflineAnnoA
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Re: Can Mycelium die of coldness ? [Re: Food]
    #486130 - 12/10/01 04:58 PM (15 years, 3 months ago)

3?C for several months.


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OfflineFood
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Re: Can Mycelium die of coldness ? [Re: Anno]
    #486218 - 12/10/01 06:35 PM (15 years, 3 months ago)

Thanks Anno !
That was a little bit of a cryptic reply though, do you mean to say that you have seen mycelium live through those conditions ?

OATIA :)


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OfflineFood
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Re: Can Mycelium die of coldness ? [Re: Food]
    #489579 - 12/13/01 08:42 AM (15 years, 3 months ago)

Ok they seem to still be the same even now many days later - could the mycelium still be alive ?


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OfflineAnnoA
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Re: Can Mycelium die of coldness ? [Re: Food]
    #489582 - 12/13/01 08:53 AM (15 years, 3 months ago)

>do you mean to say that you have seen mycelium live through those conditions ?
Yes. In my fridge.
I fruited rye grain that has been in the fridge for 1 year at that temperature.


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OfflineFood
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Re: Can Mycelium die of coldness ? [Re: Anno]
    #489585 - 12/13/01 09:05 AM (15 years, 3 months ago)

Excellent thanks .

Yay !


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Offlineaenima
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Re: Can Mycelium die of coldness ? [Re: Food]
    #489626 - 12/13/01 10:43 AM (15 years, 3 months ago)

well today decided to try cold shocking a jar to see if it wourks better, couldn't remeber if should use fridge or freezer so went with freezer, just checked, and well, it had frozen solid with a nice lamp of ice on the bottom (had added some water to jar before putting in fridge, don't know why know as pretty stoned at the time. Ohwell, will see if it will live. :)


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Re: Can Mycelium die of coldness ? [Re: aenima]
    #489641 - 12/13/01 11:02 AM (15 years, 3 months ago)

Drugs kill.(mycelium in this case)


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OfflineFood
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Re: Can Mycelium die of coldness ? [Re: Anno]
    #494645 - 12/18/01 10:58 AM (15 years, 3 months ago)

well - I came up with a theory that prehaps the mycelium was running out of air(oxygen) when it suddenly got cold so it kind of ran out of momentum and couldnt start again because of the lack of oxygen so I experimented and took the protective sticky tape off from the injection holes on one jar and lef itt for two days in the same place as the other jars and ,,,, - it worked the mycelium has begun to grow again in that jar but the others remained unchanged - so I have now removed the other peices of tape and hopefully the same will happen with them .


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OfflineGetTheFuckOut
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Re: Can Mycelium die of coldness ? [Re: Food]
    #495291 - 12/18/01 10:26 PM (15 years, 3 months ago)

man, I have this vegie cooler where I stuck dunked jars to cold shock them... The water in the jars froze around the cake. I chipped it off and put' em in a terrarium. My last cake too. It is providing me my final trip right now. My doors of preception have been blown away with hurricane force winds.


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Amazon Shop for: ½ Pint Jars, Rye Grain

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