Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Malt Extract, Peat, Perlite, Petri Dish, Portable Greenhouse, Pressure Cooker, Rye Grain, Vermiculite, pH Test Strips

Jump to first unread post. Pages: 1
Invisibledimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
A not so new tek revisited and adjusted for bulk
    #466067 - 11/21/01 11:37 PM (15 years, 23 days ago)

It involves 3 teks and works great.

This tek is a new version of BRF & vermiculite that is meant for agar instead of a syringe and involves old timers tek.

It is a very easy tek and after 36 consecutive jars with no problems I decided to share it.

The idea came from a tek posted by major millet using h2o2 and rye grain (berries) but rye grain can still be a little tricky for the beginner as agar can as well. This version is a way to use Agar and BRF & Vermiculite along with the ever faithful h2o2 (peroxide)

Supplies needed for step 1 are.

Pressure cooker (autocalve) if you have 1
1 flask 500ml or larger
MEA (malt agar extract)
1 sleeve petri dishes
1 inoculation loop
1 scalpel
Favorite spores.
Glove box (or oven)
Rubber gloves
Candy Thermometer
Clean oven mitt or a clean hand towel
Alcohol lamp (or gas stove top, cigarette lighter,etc?)

First we start with getting our MEA ready. Fill your flask (depending on size)with 500ml of water then add you MEA (about 25g) if it is a flask that does not have a plug or a cap plug the mouth with some cotton, wash cloth, (something that will keep the mea at bay so it does not spill out)

Place the media fill flask in your pressure cooker turn stove on and sterilize you MEA at 15psi for 45 minutes.

At about 35 minutes into your sterilization process get your sleeve of petri?s and place them on the oven rack. By this time you should be ready to turn your PC off allow it to start cooling when the pressure is back to zero. Get yourself washed up and put on a clean long sleeve shirt by this time you should be able to remove the lid of your PC open up the PC and remove your flask. Place it on the oven rack.
Turn your oven on LOW or the lowest set temp you have and pull the top rack about ? the way out.
Wash your hands and then put on rubber gloves then your hot mitt (if you do not have a mitt then wrap your hand towel) around the neck of the flask with your petris set up in stacks of 4 lift the cover on each dish 1 at a time and pour in your MEA do this to about 8-10 petri?s then move those to the back of the rack and allow to cool.

Now take your candy thermometer and place it in the flask and allow it to cool down until it is 120 degrees F at this time add desired amount of peroxide (rush wayne says 1 cc per 500ml MEA) I say about 3-5 cc?s per 350ml (or whatever you have left in your flask) then continue to pour the remaining petri?s dishes.

If you are confident that you poured your dishes with out allowing any contamination to get in. You may proceed to here (if you are new or not so confident) you may want to allow your dishes to sit for 3-5 days to see if anything attacks them (like green mold).
So now take your spores and open the foil,wax paper etc?and lay them on a small plate (so they will not fall through the spaces in the rack) Then flame you inoculation loop about ? of the length of the loop.
Cool your loop by sticking it in unused (this makes the loop wet and allows spores to stick to it) media then swipe some of you spores and smear them across your MEA. In a back and forth motion and then turn the dish ? of a turn and swipe back and forth again.
Follow this step until you have done 3-6 petris.

At this time you MEA that is still in the flask should be cool enough to add your h2o2 (peroxide).
Now pour your remaining dishes and mark them h2o2 or something similar.

Allow the dishes to colonize in a dark place when your dishes show growth cut some small squares of colonized agar and transfer the most rhizmoric piece to strait MEA dishes, and allow them to grow out.

When you see agar pieces with nice rhiz growth showing on them isolate them and transfer those pieces to your h2o2 MEA.
Now we wait about 1 week or so until the rhiz sections grow out until they are about ? inch from the side of the plates


The second step is to preparing your Substrate:

Now take a little bit of water and mix BRF into it until you have a sticky fairly dry paste then add your vermiculite. You want to keep it dry adding just enough water to allow it to lightly dampen everything enough to kill any mold or bacteria.
Now get your jars ready and scoop about ? to ? of substrate into each jar put the lids on and PC at 15 psi for 45 minutes.

Allow your jars to cool the take and empty, sterile, dry syringe and suck up 5cc?s of water and 5cc?s of 3% peroxide.
Now take and add about 5-8cc?s of your h2o & h2o2 shake the jars to make sure you evenly distribute the solution and your verm & BRF is nice and damp.

Now we go after the MEA h2o2 colonized petri plates and take the works to the oven and follow the oven tek then open the lids of your jars slightly and place 1-4 pieces of colonized agar in each jars.
It is not necessary with all the peroxide to be this sterile but it doesn?t hurt either.
Now allow your jar to sit somewhere dark and warm until they fully colonized.

When they are fully colonized and ready to go get your favorite bulk substrate and for our example we will use wheat straw.

Take you wheat straw and chop, shred, tear it up into small usable pieces place the straw into a pillowcase and pasteurize it at 140 degrees F for about an hour. After you are done pasteurizing it hang it up and allow it do drain until it is only slightly damp.

No get a squirt bottle and mix in 50% peroxide and 50% water. Take your almost dry straw and put it into you tray, bin, filter patch bag, and mist heavily until the straw is dripping wet.

Now here comes the fun part and this is a tek made up and written buy Old Timer and used here with his permission. This was cut and pasted from a question and answer point of view:

You can choose to use his straw tek as well.


A method I use might prove useful to you. I simply start 1/2 pint jars using traditional PF Tek. Once jars are fully colonized I get ready for straw inoculation. I soak regular old straw (which I get from a Feed & Seed) for 24 hours. After soaking I chop the straw and place in a large pasta pot. Bring to 160F for 1 hour. After pasteurization I allow straw to cool, drain and place in pans with clear "greenhouse" type lids. Take the "cake" and grind it to powder in a food processor/blender. Believe it or not the grinding does not harm the mycelium. Sprinkle the cake powder all over the straw and mix in throughly. Every minute particle of PF cake dust will become a growth point giving super fast colonization of the straw. Allow spawn to run through and case. You will get really good flushes (much more than fruiting individual cakes) and produce many more carpophores for your enjoyment. Of course
optimum humidity must be maintained throughout the entire growing cycle from inoculation to fruiting. For years I
started cultures using petri dishes, replated several times, transferred to grain and fruited strictly on manure/straw compost. Now, thanks to PF I have learned a much easier and quicker method for growing our
fungi friends using straw and cakes with no lab time at all. See, you can teach an old head new tricks.
------------------
Lots of thoughts & questions on the grinding of PF cakes. Trust me it works...
This is a repost from another forum where I am sharing the process. You may find something useful here:

Geez Hippie.... Where do I start?!

Q: Is it more effective to pasteurize wet rather than dry materials?
A: Hell if I know. I do know it's a lot easier to stir a pan of 160 degree dry soil in the oven. In short, the heat is doing its job wet or dry and it's much easier to work with dry. An additional plus is the fact that you can store the dry stuff for later use. Wet soil is much less convenient to store and is more likely to be infested with bacteria if you tried to use it later. I like being able to sterilize a batch of dry soil, use what I need and put the rest in a clean zip-loc freezer bag for later use.

Q: Why do I prefer a peat based casing mixture that requires the Ph to be adjusted?
A: Well to be honest... I've always used peat based soils for casing because that's what Bob Harris taught me to use about 25 years ago! Actually though, I really like the "consistency" of these soils. They hold moisture nicely, stay fluffy and are easy to spread over the spawn. I assume vermiculite would work but may be hard to wet evenly if spread on dry. You could probably wet it in
a bowl (like you do when fixing up PF jars) and it would do okay. It's just a matter
of what works for me as well as ease of use. As for Ph, well it's no problem to throw in a pinch of lime. It's not like you have to get a Ph pen and really adjust the medium to an exact Ph. The lime just pulls the Ph up a little and takes the acidic edge off. I have used peat soils with no lime, but I do seem to get faster/better pinning with a little lime.

Q: If one did use a 3/4th inch thick casing, what depth of substrate would you recommend?
A: Depends on how deep your container is! Seriously, I usually place about 3-4 inches of straw in the pan and case with 1/2 to 3/4 inches of soil. You don't want your straw too deep since you're wanting the spawn to colonize rapidly. A really deep container of straw tends to compact reducing spawn run plus it can get so dense it can actually mat allowing anaerobic bacteria to crop up ruining your bed. You don't need a thick layer of straw to grow an abundance of shrooms.

Q: Have you fruited directly from straw, without casing?
A: Yes.

Q: I'm told that straw doesn't need to be cased, why do you prefer to case it?
A: Open straw will pin and fruit, as will PF cakes, compost or whatever else you find to grow on. The reason for casing is easier moisture control. Pure and simple. The casing layer acts as a buffer between the air and the spawn effectively creating a micro-environment just under the casing. This protects primordia that are forming and prevents aborts due to excessive drying. Plus you can mist the casing layer without applying water directly to your spawn. The shrooms will thank you for this. By using a casing layer you can eliminate a lot of the stress involved in trying to keep bare cakes or whatever from drying. The casing makes your mushrooms a little more "forgiving", plus you don't need as wet an environment
thereby helping to reduce the growth of unwanted fungi and bacteria in your beds.


Now for Infoseeker:
Q: How many PF cakes to use?
A: I normally grind up and spread one 1/2 pint jar per square foot of bed. My beds
are generally about 3-4 inches deep but one jar should handle beds of 6 inches in depth. Be sure not to get to deep are you'll have unwanted guests from the anaerobic bacteria community. By the way, while we're discussing bacteria, please be really careful handling spawn or anything else that has become contaminated. Some of the critters you might find growing in your jars or beds can make you quite sick if you happen to breath them in or inadvertently ingest them. Dispose of any and all contaminated cultures immediately.

As for the hydrogen peroxide process for bulk spawn preparation I have to refer you to the resident expert on the subject, R. Rush Wayne, Ph.D. His booklets titled "Growing Mushrooms the Easy Way" "Cultivation with Hydrogen Peroxide" Volume I and II can be purchased from him at mycomasters.com. I have read both volumes and find that problems related to peroxide-decomposing enzymes in
organic substrates can limit your choices. He spells out ways to work around this but I have found hot water pasteurization to be an easier route. If you can swing it, set up a clean 55 gallon drum on concrete blocks and place a gas "fish cooker" under it. Make a basket out of metal hardware cloth and heat bulk quantities of straw this way. I have never needed that much straw for psilocybes but I do pasteurize straw this way for Pleurotus (oyster mushrooms), Portobellos and other gourmet shrooms that I grow.

Q: Does anybody sell ready to use compost?
A: Check with MushroomPeople and Fungi Perfecti but don't mention psychedelics
as they probably won't deal with you if you do. Personally, I would suggest making your own compost if you have access to cow manure and straw. It's very simple and I know a super quick trick to get usable compost in 7 days. I'll post the method when the "writers cramp" moves out of my fingers! Later.

Infoseeker,

If you are placing perlite under your straw it probably IS more trouble than it's worth. A good, properly prepared straw bed 3-4 inches deep shouldn't need any additional humidity or moisture retaining additives. The straw itself is moist and should hold more than enough water to produce plentiful mushrooms. My beds stay moist (especially after casing} and the mycelium grows fast and furious. You should strive for a light and airy bed with ample (but not stifling) moisture content.

Beds that I inoculate with ground up PF cakes fully colonize in 2 days to the point where you can literally lift the entire bed out as a block or brick of mycelium within 2-3 days.

Inspection under the straw reveals a moist, healthy environment with no contaminants at all. I think folks are getting too involved with environment control and missing the point (to produce carpophores). Mushroom farming is like any any other growing project. Sometimes folks get so wrapped up in creating environments they overlook the simple, common sense things. Trust me,
pasteurize some straw, drain it and place in a shallow pan with some type of lid.
Inoculate this straw with a jar or two of ground up PF cake powder as spawn and watch what happens. Let go of all the processes and procedures you've read about and let nature take its course. Please don't think I'm trying to sound like some mushroom guru or something, I'm not, I'm just relaying knowledge to you that has accumulated around me over the years. Simple methods work and simple is always better if you achieve the desired results.

If you plant a bed in the method described above you should have no need for hydrogen peroxide or any other sterilants.
------------------
Straw Bed Construction...
Place a layer of pasteurized straw about an inch thick in container. Just leave it loose, do not compact. Sprinkle 1/4 of your PF cake dust into this layer and work in. Repeat layering of straw/spawn until you reach a depth of 3-4 inches. The loose, airy straw seems to colonize faster than compacted straw. And, since you have literally provided hundreds of thousands of inocculant growth points all over the straw, the need for compacting is eliminated. Older grain spawn methods specify compaction to help the spawn to run by increasing contact between spawn and substrate. In the ground PF cake method the tiny particles of spawn stick all over the damp straw causing mycelium to sprout and run from each contact point. Let the straw grow over (2-3 days) and then case as mentioned above. Mist casing lighting a couple of times a day. You should see copius fruiting within several days to a week depending on your temps, humidity, etc.
------------------
I grow them to fruition in the same trays with "greenhouse" style plastic lids that I start them in. The trays are kept in an orchid house with controlled temps around 85-90F days and 75-80F nights. I do mist the cased trays at least twice daily. One could keep these indoors at normal room temps and expect good results.
- If you feel a need to feed your shrooms just add a little fish emulsion or bloodmeal to your water while pasteurizing the straw.

Credit needs to go to old timer, major, millet, Rush Wayne (sort of).

I hope it helps you all.

Please ask if you have questions or comments other than that ENJOY and be sure to share (it's thanksgiving)

And now if you made it this far ?well you know what to do after harvest.?


Edited by dimitri211 (11/21/01 11:39 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineEightball
whore consumer
Male User Gallery

Folding@home Statistics
Registered: 07/21/01
Posts: 3,013
Last seen: 2 years, 4 months
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466132 - 11/22/01 12:53 AM (15 years, 22 days ago)

I like it. Good job on getting all these sources together. I definitely learned quite a bit from that.


--------------------
If you're frightened of dying and you're holding on.you'll see devils tearing your life away.
But...if you've made your peace, then the devils are really angels
Freeing you from the earth.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466163 - 11/22/01 01:19 AM (15 years, 22 days ago)



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 17 days
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466259 - 11/22/01 02:37 AM (15 years, 22 days ago)

>rush wayne says 1 cc per 500ml MEA
Where does he say that? That?s way too little.

Quote from his booklet:

"To be on the safe side with my plate cultures, I use the lowest concentration of peroxide that I have found effective in agar medium, which is about 0.018%, or 6 mls per liter of medium."

This is for the 3% peroxide solution.


Edited by Anno (11/22/01 02:40 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleWorld Spirit
PNW
 User Gallery

Folding@home Statistics
Registered: 07/27/01
Posts: 9,817
Re: A not so new tek revisited and adjusted for bulk [Re: Anno]
    #466264 - 11/22/01 02:43 AM (15 years, 22 days ago)

Deleted by admin


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinestonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 6 years, 8 months
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466433 - 11/22/01 08:06 AM (15 years, 22 days ago)

Great reading...thinking of bulk...and it seems to be a good way to go!..
peace
//stonErollEr


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNewbieShroomer
member

Registered: 10/01/01
Posts: 199
Loc: San Diego California Baby...
Last seen: 14 years, 11 months
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466577 - 11/22/01 01:41 PM (15 years, 22 days ago)

Woohoo!!!

THanks Dimitri! Iread about half, it looks great, and PERFECT timing for the waters I am stepping into.

Gonna have more 1/2 & 1 pint jars colonized than I know what to do with within a week. I'm printing this post out so
I can read it further at the family Turkey day thing.

Thank you very much for taking the time and effort
to post that up for us!

Take care!


--------------------
If things get any worse, I'll have to ask you to stop helping me.

Too many freaks, not enough circuses.
..........................................
http://www.Mycotopia.net
..........................................


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
Re: A not so new tek revisited and adjusted for bulk [Re: NewbieShroomer]
    #466593 - 11/22/01 02:07 PM (15 years, 22 days ago)

If anything maybe you can read it while grace is being said.:D


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineb_butala
Stranger
Registered: 11/22/01
Posts: 3
Last seen: 15 years, 22 days
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466597 - 11/22/01 02:12 PM (15 years, 22 days ago)

You took the time to type this up and you don't give yourself credit but you give everyone else credit even though you put it together did some research and adjusted it to an easier tek.

Maybe I am old school but you should give yourself some credit.


--------------------
on cloud 9


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
Re: A not so new tek revisited and adjusted for bulk [Re: b_butala]
    #466603 - 11/22/01 02:25 PM (15 years, 22 days ago)

All I did was take 3 teks and combine them to make 1, start to finish tek.
None of the teks are mine they were thought up by others I just put them together.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible40oz
 User Gallery

Registered: 01/19/01
Posts: 30,035
Loc: Sandy Eggo. Ca.
Re: A not so new tek revisited and adjusted for bulk [Re: Anno]
    #466720 - 11/22/01 05:32 PM (15 years, 22 days ago)

great job Dimitri!!


anno...i didnt even recognize you with your new pic! lol


--------------------
:pacman: - - - -  :pill: :mushroom2: :pill2: :mushroom2: :regularshroom: :mushroomgrow: :pill: :pill2: :mushroom2: :poison:

:sun::heart::sun:

tiny_rabid_birds said:
"your avatar is dirty."


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShaw
hick

Registered: 06/28/01
Posts: 2,213
Loc: midwest, usa
Last seen: 8 months, 28 days
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #466735 - 11/22/01 05:50 PM (15 years, 22 days ago)

i learned a bit.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineV3NOM
Stranger

Registered: 05/09/13
Posts: 1
Last seen: 3 years, 6 months
Re: A not so new tek revisited and adjusted for bulk [Re: dimitri211]
    #18237296 - 05/09/13 12:21 PM (3 years, 6 months ago)

It seems this post is very old, so hopefully I will get an answer.  Anyway I am pretty new and kind of a rookie, so I just want to be sure everything is normal.  I took two of my cakes and tried this tech out.  The only thing I modified from the above tech is I did not chop the straw.  By the time it was soaked and pasteurized it was already very pliable and seemed to have a good consistency not too airy and not too compact, and even with out chopping the straw was only about 2-6 inches long.  It has been just over 2.5 days and I am seeing colonization but it is much slower than the three days stated.  I am only seeing about 20-30%.  I also do not have the resources at this time to keep it at 80 degrees so it is sitting at room temp 70-78.  Is this normal?  Anything I should change or will it just take a little longer because of my temp?  At the rate it is going I suspect full colonization in 1-2wks.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinecronicrM
Troll Eater
 User Gallery

Registered: 08/07/11
Posts: 51,812
Loc: Van Isle Flag
Last seen: 23 minutes, 7 seconds
Trusted Cultivator
Re: A not so new tek revisited and adjusted for bulk [Re: V3NOM]
    #18237343 - 05/09/13 12:29 PM (3 years, 6 months ago)

Next time just make your own thread, high 70s is great


--------------------
AMU Q N A


"It doesn't matter what I think of you, all that matters is clean spawn"




Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Malt Extract, Peat, Perlite, Petri Dish, Portable Greenhouse, Pressure Cooker, Rye Grain, Vermiculite, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* PF tek cakes to bulk or casing? (im not sure) Xtradimensional 8,113 15 06/26/13 03:33 AM
by Skyfloater
* Hydrogen Peroxide (h2o2) often propagated mistruths lysergic 1,893 11 07/13/03 06:45 PM
by Baby_Hitler
* Hydrogen peroxide (h2o2) in perlite parsonsproject 1,327 1 11/27/01 02:08 AM
by dimitri211
* Shroom Wizard Tek Revisited Anonymous 2,139 12 01/30/03 05:41 AM
by a house fly on meth
* Buckaroo Bulk Nugget Tek - grain to bulk in one sealed container
( 1 2 all )
buckaroobanzai 13,613 23 09/12/06 06:27 AM
by fingers
* Alien tek substrate as bulk? DigitalNomad 392 0 10/15/08 10:26 PM
by DigitalNomad
* m00nshines tek for growing bulk(for noobs) 2482 1,191 8 01/10/11 02:06 PM
by m00nshine
* can't find a tek to prepare bulk spawn bags. help! bent 993 5 12/14/04 12:45 PM
by tripndicular

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
4,265 topic views. 20 members, 74 guests and 27 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.095 seconds spending 0.004 seconds on 14 queries.