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Registered: 11/09/01
Posts: 215
ATTN Workman..Agar ace needed
    #457495 - 11/13/01 09:24 PM (21 years, 10 months ago)

ive been working with selectively bred strain,
although the culture is from cloned material
out of 8 cultures some have very rhizomorphic myc.
and others have intermediate linear type myc
all 8 dishes came from a single "master"
there is no sectoring within each dish they are either very rhizo or interm-linear
what would cause this?...assuming they came from a clone they (to my way of thinkin)
should all be pretty much the same....no?

Flying in a Blue Dream

Edited by TCatz (11/14/01 01:22 PM)

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Re: ATTN Workman..Agar ace needed [Re: TCatz]
    #458181 - 11/14/01 02:07 PM (21 years, 10 months ago)

They should be pretty much the same. Is your agar consistant? I have noticed that the dregs in the last few dishes of malt agar are full of dark bits and they tend to colonized thicker. Are they all poured the same depth? Thicker agar seems to work better. Are they all incubated in the same place? It is hard to tell what the problem is but, since it is a clone and the changes don't appear to be from sectoring, the cultures should still fruit well. I suspect its just different reactions to changes in environment and/or media.

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Registered: 11/09/01
Posts: 215
Re: ATTN Workman..Agar ace needed [Re: Workman]
    #458210 - 11/14/01 02:39 PM (21 years, 10 months ago)

the thickness is diff from dish to dish, i recievd this culture from 1,100miles away and
it was on pdy when i recieved i(mea now),
there were alot of changs in enviroment and media in the last 2 weeks
thank you for your promt reply on this one workman.

i just changed the heading on this post a few hours ago,
it was getting buried fast with no reply...thanks once again amigo

Flying in a Blue Dream

Edited by TCatz (11/14/01 02:41 PM)

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