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OfflineJDPhish
newbie
Registered: 05/29/00
Posts: 41
Last seen: 21 years, 8 months
Dried fruit cloning?
    #451197 - 11/08/01 12:01 AM (22 years, 3 months ago)

I guess after reading some threads on strain isolation, the best way to go about it is to take a nice looking fruit, clone it, and go from there. AFOAF recently cased some cakes and on the second flush came up with some really nice looking fruits! He's already begun drying them but was curious: if he in the "near" future got some agar plates, could he use sections from the dried mushies or is it too late at that point (have they lost their effectiveness in the drying process)?

Any help/info would be greatly appretiated. Thanks.

- Jonny D

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OfflineElektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 11 months
Re: Dried fruit cloning? [Re: JDPhish]
    #451486 - 11/08/01 08:29 AM (22 years, 3 months ago)

I would say that the cells die when dried, so you wouldn't be able to clone. The problem is that mushroom cells are not thought to outlast, like many bacteria cell do transforming themself into endospores.
For mushrooms you would have to use the spores, produced in a totally different way than in bacteria, and start again.

Elektrolurch


--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67

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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/13/99
Posts: 1,080
Last seen: 17 years, 8 months
Re: Dried fruit cloning? [Re: JDPhish]
    #451526 - 11/08/01 09:36 AM (22 years, 3 months ago)

It is possable.
It is very difficult.
you, in all likelyhood, will not succede.
I know of one person who was successful, and that was with the help of nature..


--------------------
If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.

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OfflineJDPhish
newbie
Registered: 05/29/00
Posts: 41
Last seen: 21 years, 8 months
Re: Dried fruit cloning? [Re: Elektrolurch]
    #451865 - 11/08/01 03:48 PM (22 years, 3 months ago)

Hmm.... not really the answer I was hoping for... Thanks for the info though.

- Jonny D

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Offlineanima
dusty shelf
 User Gallery

Registered: 04/30/01
Posts: 116
Loc: WAY out there.
Last seen: 10 years, 6 months
Re: Dried fruit cloning? [Re: JDPhish]
    #451980 - 11/08/01 05:48 PM (22 years, 3 months ago)

I'm pretty sure that you won't get dry mushroom tissue to "come back to life" so you can clone that particular strain, unless it is scelortia (hope I spelled that right). Although you can (with some luck) use some dry mushroom gill particles and get some spores to germinate, you would probably have problems with contam though, and it would require some agar transfers (which i do not use), and this doesn't accomplish the original goal of strain isolation.
It is best to use fresh material when you clone. I hear that you can store a syringe of mycelium water for quite some time and it will remain viable.


--------------------
It might be the early bird that gets the worm. But its the second mouse that gets the cheese

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