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OfflineOsh_Kosh_BGosh
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Registered: 10/09/01
Posts: 79
Last seen: 15 years, 2 months
rehydrating spores
    #443300 - 10/31/01 05:35 PM (15 years, 3 months ago)

is it necessary to let spores re-hydrate before innoculation when making a syringe from a spore print?


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OfflineTamrylin
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Re: rehydrating spores [Re: Osh_Kosh_BGosh]
    #443312 - 10/31/01 05:44 PM (15 years, 3 months ago)

uh. no. rehydrate? they dont need to be hydrated at all.. they just need a bit of water nearby when they germinate


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Anonymous

Re: rehydrating spores [Re: Osh_Kosh_BGosh]
    #443318 - 10/31/01 05:46 PM (15 years, 3 months ago)

YES you must rehydrate them first.


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OfflineOsh_Kosh_BGosh
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Registered: 10/09/01
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Re: rehydrating spores [Re: ]
    #443329 - 10/31/01 05:54 PM (15 years, 3 months ago)

anyone care to break the tie?


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Invisiblemrshrums
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Registered: 08/04/01
Posts: 147
Re: rehydrating spores [Re: Osh_Kosh_BGosh]
    #443344 - 10/31/01 06:11 PM (15 years, 3 months ago)

Th Psilocyber's syringe making tek in the Grow/Find section on the site suggests letting the spores re-hydrate for 12 hours. I just did this last night and made a syringe while I sterilzed my jars. The timing that way is perfect. Your jars cool for atleast 12 hours and the spores re-hydrate.


Edited by mrshrums (10/31/01 06:15 PM)


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OfflineMattyB
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Registered: 10/13/00
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Re: rehydrating spores [Re: Osh_Kosh_BGosh]
    #443478 - 10/31/01 08:21 PM (15 years, 3 months ago)

Yes, you need to rehydrate the spores, unless it's from a fresh print. All you need to do is make a spore syringe and let it sit for at least 8 hours, though 12+ is preferable.


Edited by MattyB (10/31/01 08:22 PM)


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Invisiblephrozendata
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Re: rehydrating spores [Re: MattyB]
    #443483 - 10/31/01 08:23 PM (15 years, 3 months ago)

Yes. A minimum of 12 hours. 24 hours recommended.


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OfflineOsh_Kosh_BGosh
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Re: rehydrating spores [Re: phrozendata]
    #443530 - 10/31/01 08:53 PM (15 years, 3 months ago)

that doesn't really make sense tho... once the spores get transfered from the print to syringe.. then are injected into the moist substrate along with the expelled water and are always fairly moist.. plus they are so small I really don't think so much rehydration is necessary


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Invisiblemrshrums
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Registered: 08/04/01
Posts: 147
Re: rehydrating spores [Re: Osh_Kosh_BGosh]
    #443560 - 10/31/01 09:17 PM (15 years, 3 months ago)

It may not make sense but that is what advice we have to give. If your in such a hurry just do it... What difference is 12 hours really going to make in the grand scheme of things. Do you have a strict time schedule you have to meet or something? You've spent almost more time waiting to hear what you wanted then what would have taken to re-hydrate your spores.


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Offlinefiddler4u
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Registered: 08/20/01
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Re: rehydrating spores [Re: mrshrums]
    #443576 - 10/31/01 09:29 PM (15 years, 3 months ago)

Don't take this to offence. Your a newbie I supose...or at least it seems that way. The reason of using a syringe is with a newbie in mind. Things will stay alittle cleaner for someone who dosn't have much experaince. Hope this gives you some insight.


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I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5


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Invisibleralphster44
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Re: rehydrating spores [Re: fiddler4u]
    #443587 - 10/31/01 09:38 PM (15 years, 3 months ago)

Post deleted by administrator.


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OfflineMuffinBear
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Re: rehydrating spores [Re: ralphster44]
    #444210 - 11/01/01 02:56 AM (15 years, 3 months ago)

yeup yeup, rehydrate for a day or two. specially for older prints.


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OfflineOsh_Kosh_BGosh
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Registered: 10/09/01
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Re: rehydrating spores [Re: MuffinBear]
    #444349 - 11/01/01 05:25 AM (15 years, 3 months ago)

bah growth after 3 days straight from print to syringe to jars.. no rehydration time at all


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