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Invisiblefearfect
Registered: 01/15/04
Posts: 1,845
hypo size
    #4225324 - 05/27/05 04:17 AM (12 years, 2 months ago)

what size hypodermic needles do you use? Is 25 gauge too big?


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Offlineczexysexy
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Registered: 05/24/05
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Last seen: 11 years, 11 months
Re: hypo size [Re: fearfect]
    #4225354 - 05/27/05 04:27 AM (12 years, 2 months ago)

25 gauge is smaller than the usual 18 gauge. Lower gauge = bigger, higher gauge = smaller.

25 will work fine. Spores are microscopic.


Edited by czexysexy (05/27/05 04:27 AM)


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Invisiblefearfect
Registered: 01/15/04
Posts: 1,845
Re: hypo size [Re: czexysexy]
    #4225360 - 05/27/05 04:32 AM (12 years, 2 months ago)

i should mention, they are for veins. I've never had to get them myself before so I don't know the proper gauges.


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Invisibleagar
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Re: hypo size [Re: fearfect]
    #4226351 - 05/27/05 02:14 PM (12 years, 2 months ago)

18G if you ever use LC, 20/25 will work for spores.


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OfflineMiscusi
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Re: hypo size [Re: agar]
    #4226383 - 05/27/05 02:20 PM (12 years, 2 months ago)

I use 20gx1" needles


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OfflineBlue Helix
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Re: hypo size [Re: Miscusi]
    #4228933 - 05/28/05 02:57 AM (12 years, 2 months ago)

I am using 29G needle (hair thin) for live culture (storage) to agar plate. Honestly I wouldn't have thought it would have worked until I tried it, but it works perfectly and allows me more precision in application. Using only a 100 uL drop (1/10th a CC) per agar plate the plate explodes in growth in a day or two. This live culture was constantly stirred, though, if that makes any difference.


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OfflineIGnosticAbhorI
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Registered: 11/06/04
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Re: hypo size [Re: Blue Helix]
    #4229225 - 05/28/05 05:00 AM (12 years, 2 months ago)

18g

But 20 and smaller is good for Vacutainers.

I noticed that my 18g's put holes in the rubber :frown:

-Gnostic


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Offlinebrewwhaha1
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Registered: 02/13/05
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Re: hypo size [Re: IGnosticAbhorI]
    #4229455 - 05/28/05 10:28 AM (12 years, 2 months ago)

for lots of good lc myc pickup without clogging the needle use 14gauge. There so big people wont be thinking your going to e sticking them in youryourself.


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OfflineBlue Helix
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Re: hypo size [Re: fearfect]
    #4229674 - 05/28/05 12:41 PM (12 years, 2 months ago)

I agree with IGnosticAbhorI. After poking those septums about a hundred times, you'll be glad you used a 29G needle. Here's a place that sells 100 of those 29G for $20 (probably can find it cheaper somewhere else):

http://shop.store.yahoo.com/physlabs/099611.html

They are also sold for pets in some places (for insulin delivery of course).


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