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 Tripster
200+ trips wise
Registered: 10/10/04
Posts: 275
Last seen: 11 years, 8 months
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 What's the smallest needle size ... 
#3262507 - 10/21/04 11:19 PM (13 years, 7 months ago) |
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What's the smallest needle size you can use to inoculate. I'd assume you could go small because spores are microscopic but I thought I'd ask. I used a 23g needle this last time but I have some 25g's as well that I might want to use. Last time the only one that clogged in any way was the Pink Thai Buffalo strain but it was loaded with spores to the point that I had 3-4 big clumps in there that I would have to shake up to break up in between every injection. The Pink Thai jar is the one showing the most myc growth for me so far so it definitely worked. What's the smallest you've used with success?
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You've raped! I feel dirty
It hurt! As a child
Tied down! That's a good boy
And fucked! Your own child
I scream! No one hears me
It hurt! I'm not a liar
My God! Saw you watching
Mommy why?! Your own child -Korn
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc: 
Last seen: 5 years, 4 months
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Re: What's the smallest needle size ... [Re: Tripster]
#3262676 - 10/21/04 11:44 PM (13 years, 7 months ago) |
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I've heard 25 didn't work so hot. Clogged alot. If 23 clogged even once, I wouldn't go smaller then that. I've never used less then 16 for spores but only because I've only ever used vendor made syringes. Other then that I use liquid cultures for which I use 20 gauge needles. I wouldnt go any smaller then that but thats for liquid cultures, obviously needing a much bigger needle.
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distortion
Registered: 12/11/02
Posts: 603
Loc: Murder Mitten
Last seen: 3 years, 7 months
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Re: What's the smallest needle size ... [Re: scatmanrav]
#3262738 - 10/21/04 11:56 PM (13 years, 7 months ago) |
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I don't know about the needle size. But if you didn't already know, do a search for jet dry, adding a drop of this will suspend the spores and prevent clumping.
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Think for Yourself. Question Authority.
-Timothy Leary
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Tripster
200+ trips wise
Registered: 10/10/04
Posts: 275
Last seen: 11 years, 8 months
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Re: What's the smallest needle size ... [Re: distortion]
#3262787 - 10/22/04 12:08 AM (13 years, 7 months ago) |
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Quote:
distortion said:
I don't know about the needle size. But if you didn't already know, do a search for jet dry, adding a drop of this will suspend the spores and prevent clumping.
Alright, is something you could/would add to vendor bought spores though?
--------------------
You've raped! I feel dirty
It hurt! As a child
Tied down! That's a good boy
And fucked! Your own child
I scream! No one hears me
It hurt! I'm not a liar
My God! Saw you watching
Mommy why?! Your own child -Korn
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 5 years, 4 months
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Re: What's the smallest needle size ... [Re: Tripster]
#3262816 - 10/22/04 12:13 AM (13 years, 7 months ago) |
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If the vendor made the syringe then the needle on it should be plenty big enough. I assume if your using a new needle the syringe wasn't vendor bought. If your just changing out the needle, reuse the same needle..just sterilize with flame and wipe down with alcohol.
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