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Offlinejonnyjonjonjon
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Registered: 12/06/04
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Mycellium not growing
    #4137553 - 05/05/05 02:27 PM (11 years, 7 months ago)

I made sure I did everything right, I boiled the substrate(2verm-1brf-1water) for an hour, I burned the tip of the needle before injecting, I made sure I could see the mycellium dribbling down the glass but its been 5 days now and I dont see a single bit of mycellium. I have a few jars I injected with hawaiian and amazonian about 2 weeks ago and not a fuckin thing has happened with them either unlike the ones i injected with B+(bluish green mold grew all over them). Could it be that the syringes are faulty? Ive had them since last year and they spent most of last summer in a car then I put them in the fridge.

Ive ordered a bunch of new syringes anyway I got 3 B+, 1 Mazatapec, 1 Huatla and 1 Thailand goliath, if im having trouble with hawaiian and amazonian will I have trouble with these too?


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Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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OfflineShroomKillabrew
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Registered: 04/03/05
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Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137571 - 05/05/05 02:30 PM (11 years, 7 months ago)

Boiled or pc'ed?


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Disclaimer: All my posts are purely for entertainment purposes only. Furthermore, I have NO idea what I am talking about for I have never even seen a mushroom. All picture's are taken from various sources on the internet.

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InvisibleDK1
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Registered: 11/21/04
Posts: 247
Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137578 - 05/05/05 02:30 PM (11 years, 7 months ago)

the B+ syringe was probably contamed if all your jars got green mold.


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Offlinejonnyjonjonjon
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Re: Mycellium not growing [Re: DK1]
    #4137613 - 05/05/05 02:37 PM (11 years, 7 months ago)

I boiled them for an hour, i dont have a pressure cooker.
Yea I figured the B+ syringe was contaminated seeing as it was only the B+ jars that grew mold.


--------------------
Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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Offlinethenewguy05
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Re: Mycellium not growing [Re: DK1]
    #4137618 - 05/05/05 02:37 PM (11 years, 7 months ago)

I can't stress enough that water content can mess up your whole experiment includeing but not limiting to contamination, lack of growth, slow growth, ect. if you didn't shake the syringe before inoculation chances are your shooting blanks. any number of things could have gone wrong. check the experiment and try again. i know how you feel, i used 6 cc of spores in 6 different jars and got 1 piss ass spot of mycellium in 1 jar. keep triing. better luck next time.


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OfflineHippieChick
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Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137619 - 05/05/05 02:38 PM (11 years, 7 months ago)

Being in a car last summer would lead me to believe its no good. Mycelium can reach thermal death at 106 degrees and spores can reach it at about 122 deg. With my windows up in the heat of summer I know the inside of my car gets that hot. Good Luck.
Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Offlinejonnyjonjonjon
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Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137621 - 05/05/05 02:38 PM (11 years, 7 months ago)

Another problem I forgot to mention is the color of my substrate, its white so its gonna be hard to see the mycellium, aswell it looks a bit too liquidy.


--------------------
Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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OfflineHippieChick
Chicks can do it too!
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Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137632 - 05/05/05 02:41 PM (11 years, 7 months ago)

Make sure you let your jars cool thouroughy before inoculationg. I usually wait over night.
Peace,Love,Happiness and Harmony.
:heart: HC  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Offlinejonnyjonjonjon
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Re: Mycellium not growing [Re: jonnyjonjonjon]
    #4137641 - 05/05/05 02:42 PM (11 years, 7 months ago)

" if you didn't shake the syringe before inoculation chances are your shooting blanks."
Shit I knew I was forgetting something, I still have the ends of all the syringes maybe all the spores were at the bottom.

"Mycelium can reach thermal death at 106 degrees and spores can reach it at about 122 deg."
Its not that hot where I live but im pretty sure it would have got hotter than that a few times during the summer, being in the car and all.


--------------------
Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


Post Extras: Print Post  Remind Me! Notify Moderator
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