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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar, Edible and Medicinal Mushroom Cultures on Agar   Amazon Agar, Petri Dish

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Invisibleshobimono
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Registered: 09/14/04
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Agar question
    #4064799 - 04/17/05 05:59 AM (13 years, 7 months ago)

Foaf put some mexicanaA spores on some agar and got the resulting growth.



There is some obvious contamination that looks like cobweb. What about the heavier growth? Is this mexA myc? He's seen cubie myc, but not mexA myc. Is the "myc" that is turning darker, actually mexA myc that is starting to form sclerotia? or is this whole plate contams and there is no mexA myc?
If that is good myc, then he just cuts that out and transfers to another agar dish to grow out a cleaner isolate?


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Offlineliveby
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Re: Agar question [Re: shobimono]
    #4064802 - 04/17/05 06:04 AM (13 years, 7 months ago)

isolate the contam simple ! theres the agar sandwich tek on the site which i hear is a good saver .good luck  :thumbup: :grin:


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Edited by liveby (04/17/05 06:05 AM)


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InvisibleRoadkillM
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Re: Agar question [Re: shobimono]
    #4065006 - 04/17/05 10:34 AM (13 years, 7 months ago)

Take some of the white cottony mycelium and transfer it to another petri dish.

Your picture quality is poor...but from what I see...I'd take some from the very top of the picture.

Do a few more transfers.

This is what you want.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineHotnuts
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Re: Agar question [Re: Roadkill]
    #4065034 - 04/17/05 10:48 AM (13 years, 7 months ago)

You another cool tek that was developed by Stephen Peele is the hot agar tek. You can simply pour another layer of hot agar on top of the growth layer and the mycelium will crawl through to the top layer leaving the contaminate behind on the first layer. Then simply transfer the new, clean growth to another plate or use for karo or something else.


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InvisibleRoadkillM
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Re: Agar question [Re: Hotnuts]
    #4065039 - 04/17/05 10:49 AM (13 years, 7 months ago)

Stephen Peele

:rolleyes:

That guy really is strange!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineHotnuts
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Re: Agar question [Re: Roadkill]
    #4065160 - 04/17/05 11:35 AM (13 years, 7 months ago)

LOL! Yeah but he's pretty smart. The tek is really nice. I've used to clean up cultures from prints that i've traded for on forums. So many of them are dirty. LOL
1 :grin:


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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar, Edible and Medicinal Mushroom Cultures on Agar   Amazon Agar, Petri Dish

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