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Anonymous

Preventing Contaminants before they start
    #400612 - 09/22/01 05:29 AM (15 years, 2 months ago)

Everyone post your favorite methods for cleaning, sterilizing, and preventing contamination before it begins. Or after the fact.




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InvisibleZen Peddler
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Re: Preventing Contaminants before they start [Re: ]
    #400678 - 09/22/01 10:25 AM (15 years, 2 months ago)

Pressure cook - flame needle and wipe with alcohol swab - where sterile gloves and face mask when innoculating...be paranoid



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Anonymous

Re: Preventing Contaminants before they start [Re: ]
    #401415 - 09/23/01 08:39 AM (15 years, 2 months ago)

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Offlinedarkfly
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Re: Preventing Contaminants before they start [Re: ]
    #401560 - 09/23/01 01:32 PM (15 years, 2 months ago)

What kind of stickers do you use? Those assorted smiley faces I hope!

darkfly

-- too much trippin and my soul is worn thin


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Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.


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Anonymous

Re: Preventing Contaminants before they start [Re: darkfly]
    #401612 - 09/23/01 02:28 PM (15 years, 2 months ago)

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Invisiblepuscle
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Re: Preventing Contaminants before they start [Re: ]
    #401620 - 09/23/01 02:32 PM (15 years, 2 months ago)

1) H.E.P.A. filtered room dedicated for cultivation.
2) Glovebox
3) 10% isoproponal in water.
4) Colonize jars in a closed container, i.e., cooler, rubbermaid, etc.



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Offlinetchyted
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Re: Preventing Contaminants before they start [Re: ]
    #402182 - 09/24/01 12:49 AM (15 years, 2 months ago)

my neighbor's cat insists that i do certain parts of the growing process, because she does not like to take a bath, whereas i do. she also insists on not being present during those activities. no other human beings not directly and activly engaged in the opperations are allowed in the room either.



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OfflineBBShrizz
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Re: Preventing Contaminants before they start [Re: puscle]
    #402304 - 09/24/01 04:47 AM (15 years, 2 months ago)

puscle, i do all that and more and it is AMAZING how trichoderma finds a way in but it does.





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Invisible40oz
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Re: Preventing Contaminants before they start [Re: ]
    #403299 - 09/25/01 05:05 AM (15 years, 2 months ago)

hey chem. monkey,
when you get a contam & sniff the jar.....
....if it smells like ass, you know where it came from right?
(step 8) lol!
i suggest taping a filter disk over your >> *

j/k man! lol

~fortyounces2freedom~ & ~disconnectedhaze~

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Edited by fortyounces2freedom on 09/25/01 04:06 AM.



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Anonymous

Re: Preventing Contaminants before they start [Re: 40oz]
    #403345 - 09/25/01 07:09 AM (15 years, 2 months ago)

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Invisible40oz
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Re: Preventing Contaminants before they start [Re: ]
    #404505 - 09/26/01 04:46 AM (15 years, 2 months ago)

LOL! i wasnt talking about the holes in the jar man....
i was talking about your exhaust pipe!
j/k

~fortyounces2freedom~ & ~disconnectedhaze~

get all your supplies AND spores at a discount price!!! CLICK HERE!!!


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:pacman: - - - -  :pill: :mushroom2: :pill2: :mushroom2: :regularshroom: :mushroomgrow: :pill: :pill2: :mushroom2: :poison:

:sun::heart::sun:

tiny_rabid_birds said:
"your avatar is dirty."


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Anonymous

Re: Preventing Contaminants before they start [Re: 40oz]
    #404563 - 09/26/01 08:45 AM (15 years, 2 months ago)

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OfflineJammer
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Re: Preventing Contaminants before they start [Re: ]
    #404978 - 09/26/01 04:44 PM (15 years, 2 months ago)

Can anyone explain why the PF TEK still says tp pressure cook at 15PSI for only 20 minutes? Everyone else recomends aprox. an hour of pressure cooking. It really dosent seem to me that 20 minutes could possibly be long enough.

>>Jammer>>


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>>Jammer>>


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Anonymous

Re: Preventing Contaminants before they start [Re: Jammer]
    #404986 - 09/26/01 04:49 PM (15 years, 2 months ago)

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Offlinedarkfly
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Re: Preventing Contaminants before they start [Re: ]
    #405175 - 09/26/01 07:27 PM (15 years, 2 months ago)

When doing BRF / VERM jars I load the jars up, crank the pcooker up and wait till the pressure indicator pops up and the rocker starts. Then I time for 25 minutes, then turn the heat off and take the jars out as soon as the indicator drops.


darkfly

-- too much trippin and my soul is worn thin


--------------------
Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.


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OfflineJammer
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Re: Preventing Contaminants before they start [Re: ]
    #405600 - 09/27/01 12:52 AM (15 years, 2 months ago)

It seems like I read somewhere that one could eaisly over cook things and the substract could be too dry. I take it that pressure cooking for an hour at 15 psi has not resulted in to dry a substract.....

>>Jammer>>


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>>Jammer>>


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Anonymous

Re: Preventing Contaminants before they start [Re: Jammer]
    #405613 - 09/27/01 12:59 AM (15 years, 2 months ago)

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Offlinetchyted
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Re: Preventing Contaminants before they start [Re: Jammer]
    #408750 - 09/30/01 01:28 AM (15 years, 2 months ago)

if you are following Pf tek strictly, then you are using half-pint or even smaller jars. the small size of jar allows heat to penetrate fast enogh that 20 min is all that is needed. also, the flour you get from a mill is likely allready fairly sterile due to heat in the milling process. the flour lands in the bag while still hot, and is sealed.

formost reason however, is that with the small jar, colonization time is rapid enough to beat the contams, as long as you use sufficient inocculant.

for begginers, i reccomend that you allow the licuid to trickle all the way to the bottom of the jar while innoculating in at least two holes on opposite sides of the jar.

as you get into it, you may develope your own tek, for instance, i do not bother with the holes, and i inocculate the hell out of my substrate with honey potatoe water innocculant. this takes practice, and if done sloppily, gaurantees failure. others use only one hole, and use just a tiny drop of spore water. this results in efficient use of innoculant, but takes an eternity in which contams may gain access to and ravish your virgin substrate

Edited by tchyted on 09/30/01 12:32 AM.



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