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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 12 years, 8 months
Tracing back the source of contamination
    #1687757 - 07/05/03 11:57 AM (14 years, 26 days ago)

Hey everyone, first time posting, and a bit sad that my first post should have to be in the contamination section of the forum. I did my first set of PF tek cakes and had a massive contamination problem, only managed to get 1/4 of my jars to the fruiting stage. They have flushed quiet nicely and I am very proud of them. I have created several spore syringes from the batch and am ready to start my next round. My question is, how accuratly can you trace back the source of a contamination? For example if a contaminat starts in the middle of the cake nowhere near an innoculation point can you say it is most likely something that was not killed in sterilization? Or if all the contamination started at the very top of the jar can you say it is most likely a post-innocunlation source, probably horrid blue-green mold spores floating around in the air?

And by the way, this place is great I have greatly exanded both my knowledge and motivation lurking around here.


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OfflineSev
Astropath
Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 2 years, 9 months
Re: Tracing back the source of contamination [Re: TaoinShrrom]
    #1687824 - 07/05/03 12:34 PM (14 years, 26 days ago)

Well, if you wanna be anal, do a batch of control jars.

With half of these, just let them sit on the shelf for a few weeks and see if something develops. If something does, your sterilization is off, or there's some kind of leak in the jar.

With the other half, make 'spore syringes', except just fill the syringes with tap water and PC for 20-30 minutes. After everything cools down, innoculate the other half of the jars that you made just as you would innoculate normal jars, including flaming, etc. If you get something in these jars but not the first set, there's something wrong with your sterile technique.

If -neither- of these grow anything, then the problem was with whatever was in your spore syringe. If you got it from a vendor, complain, otherwise, it could have been on the print or picked up in the process of making the syringe.


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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Offlinefactory81
enthusiast

Folding@home Statistics
Registered: 08/22/02
Posts: 314
Loc: Ibiza, Spain
Last seen: 7 years, 4 months
Re: Tracing back the source of contamination [Re: Sev]
    #1689191 - 07/06/03 01:35 AM (14 years, 25 days ago)

http://fanaticus.fungifun.org/birthday.htm

here you go, should help you trace some things....


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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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