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OfflineShroomNoob03
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MM.ORG - Question about 500ml Microbox
    #3948931 - 03/21/05 03:09 PM (12 years, 8 months ago)

If I were to use these in place of mason jars, how could I inoculate a sterilzed cake without removing the lid (and exposing the cake)??

If there is a way, I'm going to have to make a few purchases...

Here's a link if you don't know what I'm refering to:

http://www.magicmushrooms.org/products/product_info.php?cPath=34&products_id=144

Thanks,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Edited by ShroomNoob03 (03/21/05 03:35 PM)


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Invisiblecricket
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Re: MM.ORG - Question about 500ml Microbox [Re: ShroomNoob03]
    #3952789 - 03/22/05 11:07 AM (12 years, 8 months ago)

I have used them. They can be great.
I had trouble with the lids popping off during PCing.The filter will not allow pressure to pass as fast as needed.
I also had trouble inoculating.

I ended up melting a small hole it each lid and tapeing it after the PC.
This became the inoculation site. Retape after inoc.


--------------------
I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?


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OfflineShroomNoob03
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Re: MM.ORG - Question about 500ml Microbox [Re: cricket]
    #3952870 - 03/22/05 11:42 AM (12 years, 8 months ago)

Quote:

cricket said:
I have used them. They can be great.
I had trouble with the lids popping off during PCing.The filter will not allow pressure to pass as fast as needed.
I also had trouble inoculating.

I ended up melting a small hole it each lid and tapeing it after the PC.
This became the inoculation site. Retape after inoc.



If you melted the holes after you used the PC, couldn't that have lead to contamination because you exposed the cake??

Also, I'm sort of trying to avoid using tape altogether. On my first grow I used tape and I had 12 jars. That's 48 pieces of tape to peel off (I had gloves that didn't fit) and replace...It was a pain in the ass (putting it lightly).

The second time I used tin foil lids but everything got contamed...then again I did inoculate them in the bathroom (i used lysol, apparently not enough).

I figured these Microboxes might really help me out...but for that reason alone I think I'm going to avoid them.

Thanks,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Edited by ShroomNoob03 (03/22/05 11:47 AM)


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OfflineEntheo
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Re: MM.ORG - Question about 500ml Microbox [Re: ShroomNoob03]
    #3954429 - 03/22/05 05:51 PM (12 years, 8 months ago)


we have thoroughly checked these microboxes out, and we found them much better than normal chars.

the contam risk is really low when you just heat up the needle, pop it through the top and then use tape to close the tiny hole.

these pieces are also perfect for microwave sterilisation..


marvin


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OfflineShroomNoob03
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Re: MM.ORG - Question about 500ml Microbox [Re: Entheo]
    #3955721 - 03/22/05 09:54 PM (12 years, 8 months ago)

Quote:

marvindashroom said:

we have thoroughly checked these microboxes out, and we found them much better than normal chars.

the contam risk is really low when you just heat up the needle, pop it through the top and then use tape to close the tiny hole.

these pieces are also perfect for microwave sterilisation..


marvin



I wasn't sure how to use a Microwave for sterilzation so I did a search and found this...

http://www.shroomery.org/index/par/7950

Can I use this technique?

It says you must punch holes in the lid before sterilization and sterilze the container a bit before even adding the substrate. In this case I'd have to tape up each hole twice, and I'd like to avoid that.

Tape is difficult to manipulate with rubber gloves on. Not to mention dirt and bacteria can easily collect on the adhesive side, increasing the risk of contamination. I've already failed two grows and have only one syringe left, so I need to rethink my inoculation procedure before I make another attempt.

Thanks for the reply,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Edited by ShroomNoob03 (03/22/05 09:56 PM)


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Invisiblecricket
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Re: MM.ORG - Question about 500ml Microbox [Re: ShroomNoob03]
    #3955820 - 03/22/05 10:12 PM (12 years, 8 months ago)

I fought with them for a while before I learned a few tricks.
1. Punch hole in lid.
2. PC. with lid on.
3. Let cool in pc.
4. Remove lid from PC, wipe hole with Iso. alc, cover with tape.
( If you wash your hands you won't need gloves.)
5. Cool.
6. Wipe tape with alc. sterilize needle, Inuculate through the tape.
7. Cover site with new tape.
( If you wash your hands you won't need gloves.)


As the PC heats up it is puting pressure on the micobox. The pressure will equalize when the micobox reaches the same temp as the PC.
As the pc cools down the micobox and the substrate will cool at a slower rate. This will cause the pressure to build inside the micobox causing the lid to pop lose.
If you are fast and lucky you can close it after PCing, before contams get in. It is easyer to make a vent hole and cover it with alc. and tape as fast as possible.


--------------------
I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?


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