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OfflineAeolus1369
Dr. Seahorse
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Registered: 05/20/02
Posts: 367
Last seen: 8 years, 7 months
LB or THY agar?
    #3921156 - 03/15/05 03:27 PM (12 years, 6 months ago)

Does anyone know if spores will germinate on either LB (luria broth) or THY (Todd-hewitt with yeast extract) agar? These are media typically used to grow gram-positive bacteria, but I thought they might work on some level anyways (see below for compositions).

--Aeolus

LB: (amounts given as approximate percentages by weight)
Tryptone 40%
Yeast Extract 20%
NaCl 40%
Glucose 4%
MgCl2*6H20 4%
------------------Biggest concern of course would be the high salt content


THY: (numbers represent grams/liter)
Beef Heart, Infusion 3.1
Casein/Yeast Peptone 20.0
Sodium Chloride 2.0
Disodium Phosphate 0.4
Sodium Carbonate 2.5
Dextrose 2.0


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OfflinePsilygirl
cyan goddess
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Registered: 08/28/03
Posts: 4,418
Loc: PNW
Last seen: 9 months, 25 days
Re: LB or THY agar? [Re: Aeolus1369]
    #3921180 - 03/15/05 03:32 PM (12 years, 6 months ago)

not sure about THY, but you prolly don't want to grow mushrooms on LB since LB is designed to promote bacterial growth.... something I don't think you want!


--------------------
"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


Puget Sound Mycological Society


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OfflineAeolus1369
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Registered: 05/20/02
Posts: 367
Last seen: 8 years, 7 months
Re: LB or THY agar? [Re: Psilygirl]
    #3921214 - 03/15/05 03:44 PM (12 years, 6 months ago)

Ya ok, but any rich media will promote bacterial growth  :tongue:

It just has some stuff in it that makes it particularly favorable to gram-positives (eg Streptococcus).


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OfflineSuntzu
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Re: LB or THY agar? [Re: Aeolus1369]
    #3922364 - 03/15/05 08:40 PM (12 years, 6 months ago)

Spores/mycelium seem to do fine on LB agar. . .never used the other one.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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