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InvisibleNighted
Ghost

Registered: 11/10/01
Posts: 698
Loc: Funkytown
Instant Mashed Potato Agar - Who's tried it?
    #551113 - 02/14/02 07:16 PM (20 years, 7 months ago)

Here's a great sounding recipe for agar that I snagged somewhere in this forum. I'm going to try some tonight. Has anyone here used it and if so what were your experiences with it? For example, did you notice that it promoted more-less rhizomorphs than other formulas etc.

This will be used for strain selection, not storage.

Potato Flake Agar:
5 grams instant mashed potatos
10 grams dextrose [15ml corn syrup or honey]
10 grams agar
1 gram yeast extract [or any grain flour] <--I'll be substituting this with freshly powdered rye grain.
500 ml water [dung extract works fantasic for pan cyans*]
-*To make dung extract take 10-15 grams of fresh or dried dung & boil it in 1 liter of water for 5 minutes or less.
-Filter off the solids [coffee filters work great]. Toss the soild in your next substrate & use the dung extract in your culture mediums.


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Freedom defined is freedom denied.

Nighted is better than Google. Please take time to rate 5/5. Thanks!


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InvisibleNighted
Ghost

Registered: 11/10/01
Posts: 698
Loc: Funkytown
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Nighted]
    #552801 - 02/16/02 01:21 AM (20 years, 7 months ago)

Well folks, since no one seems to have any experience with this recipe, I'll make some up tonight. I'll use half with Ps. Azurescens [Thank you! You know who you are] and the other with Ps. Cyanescens.

When I have some results I'll update here and give my opinions regarding this agar in general and how it performs for strain selection. I'm pretty confident that it will be a winner with the addition of the rye. This would be a great replacement for regular PDY agar [which I hate making].


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Freedom defined is freedom denied.

Nighted is better than Google. Please take time to rate 5/5. Thanks!


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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 20 years, 5 months
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Nighted]
    #553150 - 02/16/02 08:04 AM (20 years, 7 months ago)

I've used roughly the same recipe, except i boil 4 or 5 large potatoes (chopped up) then use the water these were boiled in.


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OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 20 years, 5 months
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Nighted]
    #553434 - 02/16/02 06:48 PM (20 years, 7 months ago)

If you use fresh dung, your pH will be jacked because of the ammonia. Potato broth, dextrose, and agar are neutral, or at least very close to neutral.


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InvisibleNighted
Ghost

Registered: 11/10/01
Posts: 698
Loc: Funkytown
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Azure]
    #553456 - 02/16/02 07:31 PM (20 years, 7 months ago)

Fresh dung....now that could be a challenge to find around here. Will definitely try it if I can aquire some.


--------------------

Freedom defined is freedom denied.

Nighted is better than Google. Please take time to rate 5/5. Thanks!


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OfflinePinkStormtrooper
Jet-Puffed


Registered: 04/11/20
Posts: 218
Loc: 10001110101 Flag
Last seen: 1 year, 11 months
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Nighted]
    #26660171 - 05/09/20 11:36 PM (2 years, 4 months ago)

Quote:

Nighted said:
Here's a great sounding recipe for agar that I snagged somewhere in this forum. I'm going to try some tonight. Has anyone here used it and if so what were your experiences with it? For example, did you notice that it promoted more-less rhizomorphs than other formulas etc.
<br>
<br>This will be used for strain selection, not storage.
<br>
<br>Potato Flake Agar:
<br>5 grams instant mashed potatos
<br>10 grams dextrose [15ml corn syrup or honey]
<br>10 grams agar
<br>1 gram yeast extract [or any grain flour] <--I'll be substituting this with freshly powdered rye grain.
<br>500 ml water [dung extract works fantasic for pan cyans*]
<br>-*To make dung extract take 10-15 grams of fresh or dried dung & boil it in 1 liter of water for 5 minutes or less.
<br> -Filter off the solids [coffee filters work great]. Toss the soild in your next substrate & use the dung extract in your culture mediums.
<br>       




18 year old post?? sure why not :smile:

just poured
2.5g instant po-ta-toe
2 tsp karo
2.5g agar
dash of yeast
250ml water (not shit water just regular old water)

found 10 plates I forgot about and figured why not. the last DFA I made was stinky. tbh this dont smell great either.

They are still cooling, so I dunno yet


--------------------
"say, you got a little astroglide on your moustache"


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 05/01/13
Posts: 61,805
Loc: Milky way
Trusted Cultivator
Re: Instant Mashed Potato Agar - Who's tried it? [Re: Nighted] * 1
    #26660266 - 05/09/20 11:36 PM (2 years, 4 months ago)

This thread has been closed.

Reason:
No need to bump an old thread because you made really commonplace agar. Make a new thread in general cultivation if you need


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