|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Anonymous
|
Free agar.. now what..
#556206 - 02/19/02 11:44 AM (23 years, 5 days ago) |
|
|
I sent away for a free sample of Agar from http://www.moorhead-agar.com and a few weeks later I recieved a sample. Here's what it says:
Moorhead Agar- Agar 700B is a colloidal substance extracted from Marine Algae of the Rhodophyceae type from Gelidium genera. It is insoluble in cold water and soluble in boiling water. Moorhead Agar 700B is kosher and classified as GRAS by FDA.
Ok I'm not sure exactly how much agar this is... but I am going to estimate 15-20 grams.
Now how can I be sure how much agar to use per water? I have some disposable petri dishes so I am going to attempt my first try at agar. I am looking for a flask or something right now to sterilize it in... but how can I be sure how much is enough?
Hmm... also do I need to add anything to this agar such as malt extract? Where would I obtain that?
Any help appreciated.
Edited by Shroomism (02/19/02 11:48 AM)
|
Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 10 days, 8 hours
|
Re: Free agar.. now what.. [Re: ]
#556244 - 02/19/02 12:38 PM (23 years, 5 days ago) |
|
|
|
Incognito
member
Registered: 02/06/02
Posts: 125
|
Re: Free agar.. now what.. [Re: ]
#557726 - 02/20/02 06:16 PM (23 years, 4 days ago) |
|
|
use about 20-30 mL of water per gram, 50mL for 2grams....
each petri dish will use about 1 gram for a 1/4" layer....
use about 2/3 of that weight and substitute it with starch or dextrose or something, make PDA....if you want to add extra nutrients to the agar.
-------------------- To make a contribution to the Society for Restructuring Your Lives please contact me. We accept contributions of the following types: capital, capital producing systems, information deemed vauable to the society. Please direct all inquiries to me.
|
Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 10 days, 8 hours
|
Re: Free agar.. now what.. [Re: Incognito]
#557755 - 02/20/02 06:38 PM (23 years, 4 days ago) |
|
|
>50mL for 2grams....
That?s too much.
15-20 g agar per liter of liquid is enough.
|
Shaw

Registered: 06/27/01
Posts: 2,263
|
Re: Free agar.. now what.. [Re: ]
#557770 - 02/20/02 06:47 PM (23 years, 4 days ago) |
|
|
I believe mushmush used a beer bottle. nice narrow neck. seemed like a good idea.
--------------------
|
Anonymous
|
Re: Free agar.. now what.. [Re: Anno]
#558190 - 02/21/02 03:52 AM (23 years, 4 days ago) |
|
|
Thanks... I read in the culture mediums that straw extract can be used in the agar. Does this mean to use the water that the straw was pasteurized in as a nutrient solution? I have some now its a nice dark brown water..looks really tasty if I was mycelium.
Can I use this water to mix with the agar in the above described measurement amounts? Is that enough nutrients?
|
Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 10 days, 8 hours
|
Re: Free agar.. now what.. [Re: ]
#558218 - 02/21/02 05:53 AM (23 years, 4 days ago) |
|
|
Definitely use one of the agar recipes, the easyest is the PDA.
If you don?t have dextrose handy, you can use sacharose(household sugar).
Substitute only a part of the water you?re gona use with the straw water.
500 ml agar will give you enough agar for filling 20+ dishes. Pour app 4 mm thick.
|
Azure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 9 months
|
Re: Free agar.. now what.. [Re: Anno]
#559157 - 02/21/02 11:42 PM (23 years, 3 days ago) |
|
|
that be sucrose(glucose + fructose)...aka table sugar.
|
jonnyshaggs420
Pooh-Bah

Registered: 08/08/00
Posts: 1,965
Loc: Mid-West
Last seen: 19 years, 4 months
|
Re: Free agar.. now what.. [Re: ]
#559180 - 02/22/02 12:02 AM (23 years, 3 days ago) |
|
|
Glass snapple bottles work great for a flask. Just add your hot water and agar (optionally you can dissolve the agar in the water first then add both to the bottle) then stuff some polyfill into the mouth of the bottle, slap a little tin foil on and pressure cook the bad boy. Then when your ready to pour just take the tin foil and polyfill off.
-------------------- Vote Jonnyshaggs in the next election for GOD...Its the responsible choice
|
|