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Invisiblephrozendata
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question on strain saving
    #378589 - 08/25/01 04:19 PM (15 years, 10 months ago)

My friend received a print from the FSR and made his own syringe. Apparently he was not sterile enough because his two test jars got contaminated with the same color mold about 80& colonizating. He belives the problem to be the syringe since he had problems while making it. Is there anything he can do to save the strain? I thought perhaps he could take a healthy part of mycellium and use it to innoculate either honey water or a PF cake. Any idea?



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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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OfflineMycoGlowFlow
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Re: question on strain saving [Re: phrozendata]
    #378647 - 08/25/01 06:33 PM (15 years, 10 months ago)

the only way to get of 100% of the bacteria is to sterilize. however that would most likely kill 100% of the mycelium. i am not positive but i am pretty sure your fucked


"Its not enough, I need more. Nothing seems to Satisfy, i said. I dont't want it, i just need it. To breathe, to feel, to know i'm alive." -Maynard


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OfflineL3D
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Registered: 06/15/01
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Re: question on strain saving [Re: phrozendata]
    #380073 - 08/27/01 06:16 PM (15 years, 10 months ago)

You could try a slice of non bactira infected mycelium in a new jar of substrate. It might grow. You may want to try 2 jars that way and on 1 of the jars use a 10% h2o2 and 90% h2o to quickly soak the mycelium in to kill the Contams before putting in to the substrate. can't hurt.
What starin is it by the way. I may have a print of it i can jsut send you it will be easier.

L3D

Edited by L3D on 08/27/01 05:17 PM.



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Invisiblephrozendata
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Re: question on strain saving [Re: L3D]
    #380084 - 08/27/01 06:28 PM (15 years, 10 months ago)

Cambodian, but I dont need your print. If I really want to save it I will just spend $12 on a new sterile syringe. Thank-You for offering though.



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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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