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Amazon Shop: ½ Pint Jars, Agar, Pressure Cooker, Rye Grain

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Offlinegratefulredhead
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Filter disk placement question
    #3692238 - 01/28/05 04:50 AM (12 years, 6 months ago)

Friend of mine is working with large mouth jars with grain and filter disks. He put the filter disks under the metal lid, which had two holes in it for inoculation, and then tightened down the ring. After pressure cooking for an hour at 15 PSI, he found that the filter disks had pulled away from the side and bowed out away from the lid, thus exposing the two holes.

Any suggestions on how to remedy this problem?? Different placement?

Thanks for your help out there!


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Invisibleohmatic
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Re: Filter disk placement question [Re: gratefulredhead]
    #3692307 - 01/28/05 05:38 AM (12 years, 6 months ago)

sounds like the tyvek shrinked.
if this is the case, the pressure cooker or autoklave is reaching too
high temperature and the 'cure' would be to sterilize at lower temp.
peace ohm :mushroom2:


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InvisibleRudiger420
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Re: Filter disk placement question [Re: gratefulredhead]
    #3692480 - 01/28/05 08:54 AM (12 years, 6 months ago)

Why would you use the metal lid....Just cause you saw magash do it? Just throw the lid out and just use the filter and metal ring that screws down. Worked awesome for me. Less hassle. Even on the web site that sell em doesn't use a metal lid. PeaCE


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Offlinegratefulredhead
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Re: Filter disk placement question [Re: Rudiger420]
    #3705149 - 01/31/05 01:24 AM (12 years, 6 months ago)

Was using the metal lid to avoid TOO much evaporation... I seem to remember reading about that tip in Stametz, somewhere... not necessary??


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Offlinegratefulredhead
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Re: Filter disk placement question [Re: ohmatic]
    #3705160 - 01/31/05 01:25 AM (12 years, 6 months ago)

Hey there--thanks for the reply. Should have been 15 PSI, so that is why I'm confused... Stop cock was vibrating gently, so it doesn't seem to have been a temperature problem, as far as I can see.


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Offlinegsmith
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Re: Filter disk placement question [Re: gratefulredhead]
    #3706778 - 01/31/05 09:26 AM (12 years, 6 months ago)

if you prepare the grain and incubate properly you won't have to worry about evaporation. Trust me. Rye expands to three times it's volume when soaked, simmered and PCed correctly. All that extra volume is water. It may look dry at times but it's not. The other problem with your setup is that those two holes in the metal lid that expose the tyvek filter are obviously where you will have to inject the spores. You will have to cover those up after inocculation and you'll lose all of the gas exchange capabilities.

Just leave the metal off. And a tip for plugging those holes: Buy some all metal thumb tacks. Wrap a bunch in foil and throw them in the PC when you do your jars. After you inocculate a jar, just set one in the hole at each injection site. If it's not a snug fit, secure it down with tape that stretches across the whole lid, stuck to the metal band on both sides. I started doing this after I noticed that tape doesn't stick to the tyvek filter lids very well.


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Offlinegratefulredhead
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Re: Filter disk placement question [Re: gsmith]
    #3707454 - 01/31/05 02:24 PM (12 years, 6 months ago)

love the metal thumb tack idea. :-) Never seen that one before.

as for the two holes in the set-up--one was for inoculation, which would be taped over, and the other would allow some gas exhange.

thanks for the reply.


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OfflineLaughingJim
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Re: Filter disk placement question [Re: gratefulredhead]
    #3707497 - 01/31/05 02:36 PM (12 years, 6 months ago)

Just an observation...

"If the thumb-tack is not snug fit, just secure it with tape!"

Why not just use tape to start off?


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Offlinegsmith
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Re: Filter disk placement question [Re: LaughingJim]
    #3712195 - 02/01/05 09:49 AM (12 years, 6 months ago)

because as i said before: "I started doing this after I noticed that tape doesn't stick to the tyvek filter lids very well."

I did use tape at first, but it kept lifting up. Tape does stick to the metal band quite well however... so if you stretch the tape across the diameter of the lid, stuck to the metal band on both sides, it sort of holds the tack down and keeps it from falling out. The tacks I use seem to fit the injection hole quite snuggly, and i just use the tape as insurance to keep them from accidentally falling out. IMO it's a much better seal than tape alone. It's also a lot easier to jam a tack into the hole than it is to try and work with tape while you've got latex gloves on. the tape comes after they're out of the glove box.

and redhead, usually it's a good idea to use more than one injection point.


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Offlinegratefulredhead
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Re: Filter disk placement question [Re: gsmith]
    #3712794 - 02/01/05 12:16 PM (12 years, 6 months ago)

my friend did inject in a circular pattern (not directly in one place), but he'll take your advice into account. he's not seeing any growth after 4 days @ 86 degrees, so it looks like it is back to the drawing board. Probably some contamination from the shriveled filter disks.

He thinks he'll go for the agar development path as he did a few years back. Seemed to have better results than injecting spores directly into the rye.


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Amazon Shop: ½ Pint Jars, Agar, Pressure Cooker, Rye Grain

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