Need suggestions about peroxidated agar - Advanced Mycology

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CLuB99
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Registered: 10/26/99
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Need suggestions about peroxidated agar

#367662 - 08/06/01 11:26 AM (22 years, 4 months ago)

Yesterday i've tryied for the first time P-agar.
Since i can't find malt i used amaranth-soy agar and honey agar.
Well, i mixed, sterilized and waited to the 2 bottles cools to 50?c.
Then i mixed the peroxide and poured...and the mess begun.
the amarant soy agar condensed on the sides of the bottle while pouring, and with 500ml of agar i poured only 4 plates.
a bit better with the honey agar, less dense, with 150 ml i poured 5 plates.
Probably i should forget about amaranth-soy agar, at least for peroxidated use...
any advice from the peroxiexperts (no i can't use malt agar)?

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sylo
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Re: Need suggestions about peroxidated agar

[Re: CLuB99]
#369180 - 08/09/01 04:38 AM (22 years, 3 months ago)

It sounds like your agar cooled too much when you added the peroxide. I like to pour the plates hot and add peroxide to the plates as they cool. Mix the agar and peroxide by slowly moving the plates in a figure 8 motion.

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Anno
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Registered: 06/17/99
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Re: Need suggestions about peroxidated agar

[Re: CLuB99]
#370010 - 08/11/01 01:06 AM (22 years, 3 months ago)

Use less agar. Rush Wayne recomends using up to only 12 g per liter instead of the standard 20 g, I had good results with 15 g per liter.
Also different formulations require different amounts of agar for the same firmness.
Secondly, buy a better agar.
Bacteriological agar from sporeworks or mycosupply has an geling temperature of about only 35?C.

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CLuB99
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Registered: 10/26/99
Posts: 1,316
Loc: my mind

Re: Need suggestions about peroxidated agar

[Re: Anno]
#370142 - 08/11/01 10:01 AM (22 years, 3 months ago)

thanks anno, i think that amaranth-soy flour agar is just too solid for a peroxidated use.
i tried with honey and everything went good.

...welcome back my friend....did u had a great time in greece??

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Anno
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Re: Need suggestions about peroxidated agar

[Re: CLuB99]
#370259 - 08/11/01 04:18 PM (22 years, 3 months ago)

> did u had a great time in greece
Thank you, can?t complain ;)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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