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 zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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 Azurescens on peroxidated agar? 
#978428 - 10/20/02 11:03 PM (21 years, 10 months ago) |
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Hi,
I have just whipped up my first batch of 8 petris with:
0.5g nutritional yeast
4.5g agar agar
5.0g liquid malt extract
250 ml water (originally however I think a lot of it evaporated)
After pressure cooking for 25 mins I waited til it reached 55 deg C, then added 2ml of 6% H2O2.
I poured this into 8 90mm petris. Later I will innoculate each with a chunk of wood colonised with Ps. Azurescens.
I just want to check that Azurescens does well on peroxidated agar, that I haven't used too much peroxide, and could someone please tell me the optimum incubation temperature for azurescens.
Thanks a lot 
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zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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Re: Azurescens on peroxidated agar? [Re: zeta]
#980224 - 10/21/02 03:35 PM (21 years, 10 months ago) |
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come on folks.. answers please
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 comario2
amateur
Registered: 09/06/02
Posts: 1,352
Loc: between places
Last seen: 11 years, 7 months
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Re: Azurescens on peroxidated agar? [Re: zeta]
#980237 - 10/21/02 03:40 PM (21 years, 10 months ago) |
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azures myc (not spores) will love the C2O2  you're all good
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comario
"crusaders against emotional poverty"
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Anno
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Registered: 06/17/99
Posts: 24,167
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Re: Azurescens on peroxidated agar? [Re: zeta]
#980240 - 10/21/02 03:41 PM (21 years, 10 months ago) |
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P. azurescens does well on peroxided agar, yes, BUT:
>then added 2ml of 6% H2O2.
this would be 8 ml of 6% peroxide for 1 liter or 16 ml of 3% per liter.
Wayne recommends 6-10 ml of the 3% per liter....
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comario2
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Registered: 09/06/02
Posts: 1,352
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Re: Azurescens on peroxidated agar? [Re: Anno]
#980260 - 10/21/02 03:51 PM (21 years, 10 months ago) |
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oops, anno is more precise, as usual. you might still be ok, although the C2O2 concentration is on the high side. are you sure it was 6% and not 3%, which is the standard at least here in the us? even at 3%, 2mls per 250ml of water is high
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comario
"crusaders against emotional poverty"
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zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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Re: Azurescens on peroxidated agar? [Re: comario2]
#980311 - 10/21/02 04:18 PM (21 years, 10 months ago) |
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Yep, it was 6%.
I will just mix up another batch in case the first doesn't do well.
I have plenty of petris now.. the bastards wouldnt sell me less than 500!
Thanks for the advice
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comario2
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Posts: 1,352
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Re: Azurescens on peroxidated agar? [Re: zeta]
#980395 - 10/21/02 05:04 PM (21 years, 10 months ago) |
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so, just to be sure: at 6%, use 3mls per 1000mls of water, and so on 
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comario
"crusaders against emotional poverty"
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zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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Re: Azurescens on peroxidated agar? [Re: comario2]
#985661 - 10/23/02 03:01 AM (21 years, 10 months ago) |
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Righto, thanks for the advice people.
I have made another set of 8 with 1ml of 6% H2O2 (in 250 ml of agar).
The plates are incubating at about 20-21 degrees C, is this okay for azurescens?
How fast does azurescens mycelium typically grow?
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Zen Peddler
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Azurescens on peroxidated agar? [Re: Anno]
#985711 - 10/23/02 03:49 AM (21 years, 10 months ago) |
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Ive always used 3-4mls per 250mls of mea mixture and all my mushrooms have thrived. The mixture i use was recommended to me by a local spawn company and they have never had mutations as a result of this ratio.
If your attempting to use virgin spawn or colonised woodchips or clones on your agar, i always up the h202 to about 4-5mls per 250mls.
I never ever use less than 3mls of h202 for 250mls.
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comario2
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Re: Azurescens on peroxidated agar? [Re: Zen Peddler]
#986136 - 10/23/02 09:28 AM (21 years, 10 months ago) |
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you're talking about 6% concentration right? i guess that must be the standard for your part of the world)
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comario
"crusaders against emotional poverty"
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Anonymous
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 Re: Azurescens on peroxidated agar? [Re: zeta]
#1057783 - 11/16/02 08:06 AM (21 years, 9 months ago) |
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hey zeta! how is this coming along 4 u? did u figure out what ideal incubation temps are? i hope this is working out 4 u... please post how ur doing if u feel it's worthwhile... peace
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zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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Re: Azurescens on peroxidated agar? [Re: Anonymous]
#1058453 - 11/16/02 04:09 PM (21 years, 9 months ago) |
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Hi, The azure is coming along well thanks.
I'm incubating at about 22 degrees (I think), in my hot water cupboard.
There is little difference between the correctly-peroxidated and over-peroxidated petris.
Most are fully colonised like this. I have sectored a few chunks out onto new petris to try and get some more rhizomorphic growth.
I have also inoculated a few jars of rye grain with agar wedges.
I'm not planning to fruit off the grain (obviously). I think I will spread it out in a tray, cover it with a layer of wet cardboard, wait til the cardboard is fully colonised then cover with soaked woodchips. Finding the right woodchips is a bit of a problem for me at the moment though. Can anybody tell me whether I should shake these jars or is azure mycelium too fragile? Thanks
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