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Invisiblezeta
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Registered: 05/25/02
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Azurescens on peroxidated agar?
    #978428 - 10/21/02 01:03 AM (18 years, 10 months ago)

Hi,
I have just whipped up my first batch of 8 petris with:
0.5g nutritional yeast
4.5g agar agar
5.0g  liquid malt extract
250 ml water (originally however I think a lot of it evaporated)
After pressure cooking for 25 mins I waited til it reached 55 deg C, then added 2ml of 6% H2O2.
I poured this into 8 90mm petris. Later I will innoculate each with a chunk of wood colonised with Ps. Azurescens.
I just want to check that Azurescens does well on peroxidated agar, that I haven't used too much peroxide, and could someone please tell me the optimum incubation temperature for azurescens.
Thanks a lot  :wink:   


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Invisiblezeta
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Re: Azurescens on peroxidated agar? [Re: zeta]
    #980224 - 10/21/02 05:35 PM (18 years, 10 months ago)

come on folks.. answers please


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Offlinecomario2
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Re: Azurescens on peroxidated agar? [Re: zeta]
    #980237 - 10/21/02 05:40 PM (18 years, 10 months ago)

azures myc (not spores)  will love the C2O2  :laugh: you're all good 


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OfflineAnnoA
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Re: Azurescens on peroxidated agar? [Re: zeta]
    #980240 - 10/21/02 05:41 PM (18 years, 10 months ago)

P. azurescens does well on peroxided agar, yes, BUT:

>then added 2ml of 6% H2O2.

this would be 8 ml of 6% peroxide for 1 liter or 16 ml of 3% per liter.

Wayne recommends 6-10 ml of the 3% per liter....


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Offlinecomario2
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Re: Azurescens on peroxidated agar? [Re: Anno]
    #980260 - 10/21/02 05:51 PM (18 years, 10 months ago)

oops, anno is more precise, as usual. you might still be ok, although the C2O2 concentration is on the high side. are you sure it was 6% and not 3%, which is the standard at least here in the us? even at 3%, 2mls per 250ml of water is high


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Invisiblezeta
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Re: Azurescens on peroxidated agar? [Re: comario2]
    #980311 - 10/21/02 06:18 PM (18 years, 10 months ago)

Yep, it was 6%.
I will just mix up another batch in case the first doesn't do well.
I have plenty of petris now.. the bastards wouldnt sell me less than 500!
Thanks for the advice


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Offlinecomario2
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Re: Azurescens on peroxidated agar? [Re: zeta]
    #980395 - 10/21/02 07:04 PM (18 years, 10 months ago)

so, just to be sure: at 6%, use 3mls per 1000mls of water, and so on :smile: 


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Invisiblezeta
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Re: Azurescens on peroxidated agar? [Re: comario2]
    #985661 - 10/23/02 05:01 AM (18 years, 10 months ago)

Righto, thanks for the advice people.
I have made another set of 8 with 1ml of 6% H2O2 (in 250 ml of agar).
The plates are incubating at about 20-21 degrees C, is this okay for azurescens?
How fast does azurescens mycelium typically grow?


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InvisibleZen Peddler
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Re: Azurescens on peroxidated agar? [Re: Anno]
    #985711 - 10/23/02 05:49 AM (18 years, 10 months ago)

Ive always used 3-4mls per 250mls of mea mixture and all my mushrooms have thrived. The mixture i use was recommended to me by a local spawn company and they have never had mutations as a result of this ratio.
If your attempting to use virgin spawn or colonised woodchips or clones on your agar, i always up the h202 to about 4-5mls per 250mls.
I never ever use less than 3mls of h202 for 250mls.


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Offlinecomario2
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Re: Azurescens on peroxidated agar? [Re: Zen Peddler]
    #986136 - 10/23/02 11:28 AM (18 years, 10 months ago)

you're talking about 6% concentration right? i guess that must be the standard for your part of the world)


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Anonymous

Re: Azurescens on peroxidated agar? [Re: zeta]
    #1057783 - 11/16/02 10:06 AM (18 years, 10 months ago)

hey zeta! how is this coming along 4 u? did u figure out what ideal incubation temps are? i hope this is working out 4 u... please post how ur doing if u feel it's worthwhile... peace





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Invisiblezeta
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Re: Azurescens on peroxidated agar? [Re: Anonymous]
    #1058453 - 11/16/02 06:09 PM (18 years, 10 months ago)

Hi, The azure is coming along well thanks.
I'm incubating at about 22 degrees (I think), in my hot water cupboard.
There is little difference between the correctly-peroxidated and over-peroxidated petris.

Most are fully colonised like this. I have sectored a few chunks out onto new petris to try and get some more rhizomorphic growth.

I have also inoculated a few jars of rye grain with agar wedges.

I'm not planning to fruit off the grain (obviously). I think I will spread it out in a tray, cover it with a layer of wet cardboard, wait til the cardboard is fully colonised then cover with soaked woodchips. Finding the right woodchips is a bit of a problem for me at the moment though. Can anybody tell me whether I should shake these jars or is azure mycelium too fragile? Thanks




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