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InvisibleCLuB99
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Alternatives formulas for peroxidated agar
    #360133 - 07/24/01 05:59 PM (16 years, 3 months ago)

Hi to all....
I'm waiting for a LFH, but in the meantime i wish to experiment with peroxiagar but my problem is that here i can't find malt extract.
So my question is: wich one of this formulas can i use with peroxide without decomposing it??
potato dextrose agar, amaranth- soy flour agar, honey agar, beer agar.

really thanks for your help


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OfflineAnnoA
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Re: Alternatives formulas for peroxidated agar [Re: CLuB99]
    #360548 - 07/25/01 06:36 AM (16 years, 3 months ago)

Basically any of the aforementioned formalations.

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Re: Alternatives formulas for peroxidated agar [Re: CLuB99]
    #360583 - 07/25/01 08:57 AM (16 years, 3 months ago)

why can't you find malt extract?

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Re: Alternatives formulas for peroxidated agar [Re: CLuB99]
    #360585 - 07/25/01 09:16 AM (16 years, 3 months ago)

Here is the perhaps the easiest culture formal that I have found. It works great with H2O2.

5 grams of potato flakes (instant mash potatos )
10 grams of Dextrose (15ml corn syurp or honey)
10 grams of Agar Agar
1 gram of yeast extract (or any grain flour)
500 ml of water (Dung extract works fantasic for pan cyans)
{To make dung extract take 10 to 15 grams of either fresh or dried dung and boil it in 1 liter of water,for 5 min. or less. Filter off the soilds, (coffee filters work great). toss the soild in your next substrate, and use the dung extract in your culture mediums) }

Hope this helps


Edited by Tryp'er on 07/25/01 09:34 AM.



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OfflineAnnoA
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Re: Alternatives formulas for peroxidated agar [Re: CLuB99]
    #360601 - 07/25/01 10:44 AM (16 years, 3 months ago)

> use with peroxide without decomposing it??
It?s important to add the peroxide after pressure cooking once the temperature has fallen under 50?C.

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