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OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Liquid peptone mix? * 1
    #3296282 - 10/30/04 03:46 AM (19 years, 4 months ago)

Do anyone use peptone in liquid medium? If so, how do you mix it?

YSr

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OfflineParsifal
Shrrroomozaurus

Registered: 03/02/04
Posts: 9
Loc: Slovenia
Last seen: 17 years, 11 months
Re: Liquid peptone mix? [Re: YSr] * 1
    #3296342 - 10/30/04 04:43 AM (19 years, 4 months ago)

I am using it sometimes. simply adding 1-2g per liter of standard Malt or Dextorse broth. Mycelium grows faster& more agressive - healty.

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OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Re: Liquid peptone mix? [Re: Parsifal] * 1
    #3296372 - 10/30/04 04:57 AM (19 years, 4 months ago)

4% dextrose and 0,1 - 0,2% peptone?
Is 0,3% too much?

YSr

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Invisiblenoxy
Dr
 User Gallery

Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
Re: Liquid peptone mix? [Re: YSr] * 1
    #3297481 - 10/30/04 01:07 PM (19 years, 4 months ago)

top end would be 0.5 %

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OfflineYSr
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Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Re: Liquid peptone mix? [Re: noxy] * 1
    #3300027 - 10/31/04 02:01 AM (19 years, 4 months ago)

What is the best liquid recipe for Cubensis?

YSr

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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: YSr] * 1
    #3326239 - 11/05/04 10:08 PM (19 years, 4 months ago)

I strongly recomend using peptone. I use liquid media to expand all my cultures.

Mass spore inoculation
Fruiting
culture best fruits and grow out in liquid culture, used to inoculate more substrate

I use maltose, yeast extract, and peptone
Add 10% glycerol for preservation when you want to freeze the culture

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OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Re: Liquid peptone mix? [Re: ryan] * 1
    #3326860 - 11/06/04 04:29 AM (19 years, 4 months ago)

Thank you.
How much maltose and peptone?

YSr

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OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Re: Liquid peptone mix? [Re: YSr] * 1
    #3326861 - 11/06/04 04:32 AM (19 years, 4 months ago)

And where do you buy maltose?
Could I use this one? http://www.iherb.com/carbopowder.html

YSr

Edited by YSr (11/06/04 04:35 AM)

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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: YSr] * 1
    #3359256 - 11/14/04 09:51 AM (19 years, 4 months ago)

If I remember correctly I use 1g peptone / liter. Ill have to look up how much maltose I use, but I think about 5g/l. 1g of the yeast extract as well per liter

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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: ryan] * 1
    #3389889 - 11/20/04 04:07 PM (19 years, 4 months ago)

I now have the correct numbers:

20g Maltose (may use tan brewers malt -from beer brewing stores)
5g Peptone
2g yeast extract

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OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 8 months
Re: Liquid peptone mix? [Re: ryan] * 1
    #3392216 - 11/21/04 04:09 AM (19 years, 4 months ago)

Thank you!

YSr

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