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OfflineYSr
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Registered: 04/13/04
Posts: 81
Last seen: 11 years, 3 months
Liquid peptone mix?
    #3296282 - 10/30/04 05:46 AM (12 years, 11 months ago)

Do anyone use peptone in liquid medium? If so, how do you mix it?

YSr


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OfflineParsifal
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Registered: 03/02/04
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Loc: Slovenia
Last seen: 11 years, 6 months
Re: Liquid peptone mix? [Re: YSr]
    #3296342 - 10/30/04 06:43 AM (12 years, 11 months ago)

I am using it sometimes. simply adding 1-2g per liter of standard Malt or Dextorse broth. Mycelium grows faster& more agressive - healty.


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OfflineYSr
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Posts: 81
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Re: Liquid peptone mix? [Re: Parsifal]
    #3296372 - 10/30/04 06:57 AM (12 years, 11 months ago)

4% dextrose and 0,1 - 0,2% peptone?
Is 0,3% too much?

YSr


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Invisiblenoxy
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Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
Re: Liquid peptone mix? [Re: YSr]
    #3297481 - 10/30/04 03:07 PM (12 years, 11 months ago)

top end would be 0.5 %


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OfflineYSr
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Re: Liquid peptone mix? [Re: noxy]
    #3300027 - 10/31/04 04:01 AM (12 years, 11 months ago)

What is the best liquid recipe for Cubensis?

YSr


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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: YSr]
    #3326239 - 11/06/04 12:08 AM (12 years, 11 months ago)

I strongly recomend using peptone. I use liquid media to expand all my cultures.

Mass spore inoculation
Fruiting
culture best fruits and grow out in liquid culture, used to inoculate more substrate

I use maltose, yeast extract, and peptone
Add 10% glycerol for preservation when you want to freeze the culture


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OfflineYSr
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Re: Liquid peptone mix? [Re: ryan]
    #3326860 - 11/06/04 06:29 AM (12 years, 11 months ago)

Thank you.
How much maltose and peptone?

YSr


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OfflineYSr
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Registered: 04/13/04
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Re: Liquid peptone mix? [Re: YSr]
    #3326861 - 11/06/04 06:32 AM (12 years, 11 months ago)

And where do you buy maltose?
Could I use this one? http://www.iherb.com/carbopowder.html

YSr


Edited by YSr (11/06/04 06:35 AM)


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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: YSr]
    #3359256 - 11/14/04 11:51 AM (12 years, 11 months ago)

If I remember correctly I use 1g peptone / liter. Ill have to look up how much maltose I use, but I think about 5g/l. 1g of the yeast extract as well per liter


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Invisibleryan
Member since 1997
Registered: 03/01/01
Posts: 111
Re: Liquid peptone mix? [Re: ryan]
    #3389889 - 11/20/04 06:07 PM (12 years, 10 months ago)

I now have the correct numbers:

20g Maltose (may use tan brewers malt -from beer brewing stores)
5g Peptone
2g yeast extract


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OfflineYSr
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Re: Liquid peptone mix? [Re: ryan]
    #3392216 - 11/21/04 06:09 AM (12 years, 10 months ago)

Thank you!

YSr


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