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Invisiblewind_wraith
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Registered: 05/16/01
Posts: 1,200
plastic baggie tek?
    #326983 - 05/27/01 02:03 PM (15 years, 9 months ago)

Hi hi hi there

I'm a newbie and am just getting ready to case my first (successful) batch of Cubensis. Meanwhile I've been experimenting with some "alternative" growing methods, like using cooked grass as a substrate (it works well) and now microwave sterilization. So last night I filled a big Ziploc bag with the usual BRF mix, nuked it for ten minutes and innoculated it by poking the needle through the plastic and then taping up the holes. It seemed a bit sloppy, though, and the level of sterility was questionable at best, so I'm wondering if there are more refined teks out there; someone mentioned a PF method here a few days ago, anyone know where I can find this or other microwave teks? Thanks,

WW



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InvisibleTripsAreForKids
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Registered: 04/23/01
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Re: plastic baggie tek? [Re: wind_wraith]
    #327068 - 05/27/01 04:50 PM (15 years, 9 months ago)

goto the growing section here at the shroomery.. there are all sorts of grow guides and teks and stuff....

Keep Trippin' and Blazin'

-=SporeWorks=-


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Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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InvisibleTeragon
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Registered: 02/21/01
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Re: plastic baggie tek? [Re: wind_wraith]
    #327366 - 05/27/01 09:41 PM (15 years, 9 months ago)

Hey Wind_Wraith-
Try what I did with the microwave. I took two Ziploc plastic containers(blue tops), and filled them like PF Tek(BRF/V) with the vermiculite barrier. Don't forget to put 4 holes in the corners for innoculation. I then put one of those in a plastic bowl with some water in the bottom(a couple of mm), and also boiled some water in the teapot. Once the water steamed I poured it in a mug a put that in the microwave with the bowl. That was to make sure there was no loss of moisture(commonly a problem with microwave sterilization). I then turned on themicrowave for 8 mins on high(do only one container at a time). The top pops off during it, but after it was done, I reached in and taped up the holes while pushing the lid back on tightly(to avoid contams). Wait for them to cool and then innoculate. I currently have two of them growing, and they are doing real well. Good luck!

Peace
Teragon



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OfflineDisc0rd
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Re: plastic baggie tek? [Re: Teragon]
    #327669 - 05/28/01 08:17 AM (15 years, 9 months ago)

Teragon: As I've got a lot of those Ziploc containers lying around, your idea sounds like something I might like to try next. (Presently I've got some MMGG-style jars started.) I'm quite new at growing, though, so I've got a couple questions. How big are your containers, and how much longer than usual do you expect the colonization will take? When the mycelium is finished growing round the substrate, what will you do for birthing (e.g. take off the lid and set the container in a terrarium / put the square "cake" in a terrarium)?
Good luck with your tek. :)

- disc0rd



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InvisibleTeragon
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Re: plastic baggie tek? [Re: Disc0rd]
    #327717 - 05/28/01 11:38 AM (15 years, 9 months ago)

Disc0rd-
They are maybe 5x5" or so on top? About 2 inches tall. I'm guessing because I'm too lazy to go measure. I'm sure you know which ones I'm talking about(they have blue tops). Colonization time will depend on the strain, but mine are about 3 weeks old, and they are almost done. I say probably 3-4 weeks for full colonization. You could just birth them in a terrarium, but I am growing mine invitro. It's a matter of choice(and if you've got the space). Good luck!

Peace
Teragon



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Invisiblewind_wraith
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Re: plastic baggie tek? [Re: wind_wraith]
    #327952 - 05/28/01 05:03 PM (15 years, 9 months ago)

Awesome, I'm going to try it. Are these Ziploc containers transparent so you can see your mycelium growing, or a bit foggy like Tupperware containers? This seems like a real step up from mason jars and pressure cookers, as long as it's comparably sterile, anyway -- the ultimate lazy man's tek :-)



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InvisibleTeragon
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Re: plastic baggie tek? [Re: wind_wraith]
    #327973 - 05/28/01 05:33 PM (15 years, 9 months ago)

Wind_Wraith-
Yeah they are transparent. The top is transparent blue, and the rest is clear. I would say that the microwave definitely works well, but it doesn't beat a pressure cooker. Say your doing 12 jars/containers, then in the microwave you have to do one at a time(8 mins each) That's 12 x 8 = 96 mins. In a pressure cooker you can do all 12 at once for about 45 mins. That is much better than the microwave. But it's great if you don't have a pressure cooker. Good luck!

Peace
Teragon



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Invisiblewind_wraith
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Re: plastic baggie tek? [Re: Teragon]
    #330760 - 05/31/01 04:26 PM (15 years, 9 months ago)

Ok, I microwave-sterilized a new batch last night, using a couple of the Ziploc containers and also a large shallow tupperware. The Ziplocs I nuked for eight minutes as you suggested, but the first time I tried omitting the water and ended up with a bunch of nasty-smelling fried substrate and melted plastic... the second time I sat the container in 1/2" of water and had no problems -- I think the water's even more important as a coolant than as a way to keep evaporation down. I fitted about two jars' worth of substrate into each of the Ziplocs, and eight into the tupperware, then inoculated at four and seven points, respectively. I also added a layer of saran wrap between the substrate and the lids, to get more of a hermetic seal on the containers. Incidentally, my plastic baggie culture is doing well, so far without contams :-)



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OfflineAshaman
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Re: plastic baggie tek? [Re: wind_wraith]
    #330767 - 05/31/01 04:36 PM (15 years, 9 months ago)

wow wind, that sounds great, keep us updated. Im surprised theres not more interest in this, I havent heard much about microwave style.



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InvisibleHippie3
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Re: plastic baggie tek? [Re: Ashaman]
    #331393 - 06/01/01 09:00 AM (15 years, 9 months ago)

that's because microwaves can be pretty hit-or-miss about sterilization, so most prefer pressure cooking.

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Invisiblewind_wraith
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Re: plastic baggie tek? [Re: Hippie3]
    #331793 - 06/01/01 07:05 PM (15 years, 9 months ago)

Hmm, I'm very new to this, but wouldn't any tek which heats the substrate uniformly to 100?C sterilize it equally well? If contams develop, I'd think it was because the substrate was not sufficiently heated (particularly at the center) or because of unsterile conditions afterwards (loose-fitting lids, dirty needles, etc.).



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InvisibleTripsAreForKids
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Re: plastic baggie tek? [Re: wind_wraith]
    #331947 - 06/01/01 10:56 PM (15 years, 9 months ago)

after you sterilized it, let it sit for a few days or something to see if there is any contams, if there are, then just try it for a longer time, its better than injecting with spores and messing up and not know whether the sterilization was not good enuf or if it was contamed syringe, etc etc....

Keep Trippin' and Blazin'

-=SporeWorks=-


--------------------
Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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Offlinepyromaniac
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Re: plastic baggie tek? [Re: wind_wraith]
    #332041 - 06/02/01 01:55 AM (15 years, 9 months ago)

Well it seems that if 2 jars worth fit into one of these containers, then 10 of these each week would be like 20 jars each week. If it takes 4 weeks to colonize fully, then 40 containers will allow you to make about 20 jars worth each week. Nobody could need that much but you could organize it like this to where each week you have another batch of fully colonized containers, so you dont have to wait 4 weeks each time, and only a couple days between each harvest. Plus you could be growing a bunch of different species at once and keep rotating in new ones.

Im going to have to try something like this. Not such a large scale, but a system set up like this could make it a lot easier to produce a lot of different strains in a shorter amount of time.

---Pyromaniac---


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OfflineTaz
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Re: plastic baggie tek? [Re: Hippie3]
    #332042 - 06/02/01 01:57 AM (15 years, 9 months ago)

:cool:

"Out of chaos...comes order...."


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Invisiblewind_wraith
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Re: plastic baggie tek? [Re: pyromaniac]
    #332723 - 06/03/01 12:05 AM (15 years, 9 months ago)

Yeah, you could do that with jars, too, but the microwave method seems a lot more convenient to me ('specially since I don't have a pressure cooker), plus the containers are cheap and disposable. Seems like a good idea for a bulk tek as well, using the larger containers -- only problem is, if your culture is contaminated then you've just wasted at least half of a spore syringe on a single container, but I expect that this is the case for all bulk methods...



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Offlinepyromaniac
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Re: plastic baggie tek? [Re: wind_wraith]
    #332842 - 06/03/01 04:13 AM (15 years, 9 months ago)

Ya you could do that with the jars but these are larger and just seem so much easier.

---Pyromaniac---


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Support the FSR!


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