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InvisibleGreat_Cthulhu
enthusiast

Registered: 05/21/01
Posts: 311
Loc: R'lyeh
Microwave sterilization good/bad?
    #322203 - 05/21/01 01:55 AM (22 years, 11 months ago)

How reliable is sterilizing with a microwave oven? How long would one wish to run it to sterilize such things as substrate and casing. Any help is appreciated.



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"That is not dead which may eternal lie..and with strange eons even death may die."
Vote Great Cthulhu for President. Why vote for a lesser evil?

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OfflinewhyIdied
member

Registered: 04/26/01
Posts: 88
Loc: The exact center of the e...
Last seen: 22 years, 9 months
Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
    #322391 - 05/21/01 12:13 PM (22 years, 11 months ago)

I've been gambling with microwave steralization: I just put things in there for 5 minutes, open the door for 1 minute and then repeat until I've nuked the fucker for 20 minutes or so. I don't like letting it go on continuously, because I'm affraid it could reach unpleasent temps.

But I don't see how contams could escape the destructive power of EMR radiation that strong! die die die

...slow moves, sweet feet...


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...slow moves, sweet feet...

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OfflineMAIA
World-BridgerKartikeya (DftS)
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Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 9 days
Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
    #322436 - 05/21/01 01:20 PM (22 years, 11 months ago)

I'm trying a better technique, before going to the microwave let the jars freeze inside your refrigerators freezer for a day, them set your microwave to half power, put jars inside and defreeze for about 2 or 3 minutes, now increase power to 3/4 of full capacity for about 2 minutes, set it back to 1/2 or defrost if you see there's too much heat inside, repeat untill ice is melted or heat it a bit more.
BTW, here you have some pics of colonized honeywater for you too see. Water was sterilized using this method.

Cheers,
MAIA

== :) Spread the Spores :) ==


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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OfflineMAIA
World-BridgerKartikeya (DftS)
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Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 9 days
Re: Microwave sterilization good/bad? [Re: MAIA]
    #322440 - 05/21/01 01:24 PM (22 years, 11 months ago)
Log in to view attachment

Trying pic again.
See'ya.

== :) Spread the Spores :) ==


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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Offlinecbee
journeyman

Registered: 04/26/01
Posts: 49
Last seen: 22 years, 30 days
Re: Microwave sterilization good/bad? [Re: MAIA]
    #322534 - 05/21/01 03:10 PM (22 years, 11 months ago)

can u use microwave sterilization to sterilize substrate rye jars?



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OfflineAleon_Niir
Stranger
Registered: 05/20/01
Posts: 5
Loc: South America/Scandinavia
Last seen: 22 years, 8 months
Growing in honeywater? [Re: MAIA]
    #322560 - 05/21/01 03:48 PM (22 years, 11 months ago)

How does it work to grow in honeywater? Are there any guids of it? What are the opinios of you who have tried it?

I would like to know everything about the honywater method if it?s good.

/Aleon Niir


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/Aleon Niir

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InvisibleTeragon
Noddy

Registered: 02/20/01
Posts: 36,253
Loc: Lost in the Patterns
Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
    #322589 - 05/21/01 04:17 PM (22 years, 11 months ago)

I just recently sterilized two Ziploc containers in the microwave, PF style. I then innoculated them, and now they show great signs of strong mycelium growth. I sterilized them in the microwave for 8 mins on high, sitting in a layer of water about 5 mm deep. I also boiled water in a teapot and poured it into a mug when it was steaming and set that into the microwave so that the containers wouldn't lose any moisture. The tops popped off during sterilization, but I pushed them back on, and taped up the holes before I took it from the microwave to avoid contams. Let it cool down(overnight), and then innoculate. Make sure you put holes in the top before putting in the microwave. Enjoy!



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Offlinecbee
journeyman

Registered: 04/26/01
Posts: 49
Last seen: 22 years, 30 days
Re: Microwave sterilization good/bad? [Re: Teragon]
    #322994 - 05/21/01 11:52 PM (22 years, 10 months ago)

so can u use it to sterilize grains like rye, bird seed, etc?



--------------------
:laugh: <-- he's happy cause he got laid last night...
Visit the Free Spore Ring.. support the happy face
http://go.to/FreeSporeRing

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OfflineMAIA
World-BridgerKartikeya (DftS)
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Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 9 days
Re: Growing in honeywater? [Re: Aleon_Niir]
    #323331 - 05/22/01 12:54 PM (22 years, 10 months ago)

"I" did it this way:
1- Get a 250ml jar with plastic lid, punch a hole on lid and tape it.
2- Fill the jar with destiled water
3- Put 1/2 tea spoon with honey into the jar and mix it.
4- Microwave with it for 5 minutes, max power.
5- Take jar, put it inside freezer and let it freeze (it takes some time depending on freezer).
6- Take jar out of freezer and put it inside some container with water for 5 minutes to defreeze the glass.
7- Microwave it again (5 min) and then let it rest untill it's cold.
8- Grab spore syringe and inoculate with 1cc using the hole on lid.
9- Let jar rest on a heating pad at 75F or 80F.

Steps 5,6 and 7 are optional, i use this freezer technique when microwaving just to reduce contam. probabilities.
After 3 days you'll see mycelium growing inside the jar.
Good luck.

Cheers,
MAIA



== :) Spread the Spores :) ==


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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