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 Great_Cthulhu
enthusiast
Registered: 05/21/01
Posts: 311
Loc: R'lyeh
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 Microwave sterilization good/bad? 
#322203 - 05/21/01 01:55 AM (22 years, 4 months ago) |
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How reliable is sterilizing with a microwave oven? How long would one wish to run it to sterilize such things as substrate and casing. Any help is appreciated.
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"That is not dead which may eternal lie..and with strange eons even death may die."
Vote Great Cthulhu for President. Why vote for a lesser evil?
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 whyIdied
member
Registered: 04/26/01
Posts: 88
Loc: The exact center of the e...
Last seen: 22 years, 2 months
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Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
#322391 - 05/21/01 12:13 PM (22 years, 4 months ago) |
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I've been gambling with microwave steralization: I just put things in there for 5 minutes, open the door for 1 minute and then repeat until I've nuked the fucker for 20 minutes or so. I don't like letting it go on continuously, because I'm affraid it could reach unpleasent temps.
But I don't see how contams could escape the destructive power of EMR radiation that strong! die die die
...slow moves, sweet feet...
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...slow moves, sweet feet...
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MAIA
World-BridgerKartikeya (DftS)
 
Registered: 04/27/01
Posts: 7,391
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 15 hours, 6 minutes
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Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
#322436 - 05/21/01 01:20 PM (22 years, 4 months ago) |
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I'm trying a better technique, before going to the microwave let the jars freeze inside your refrigerators freezer for a day, them set your microwave to half power, put jars inside and defreeze for about 2 or 3 minutes, now increase power to 3/4 of full capacity for about 2 minutes, set it back to 1/2 or defrost if you see there's too much heat inside, repeat untill ice is melted or heat it a bit more.
BTW, here you have some pics of colonized honeywater for you too see. Water was sterilized using this method.
Cheers,
MAIA
== :) Spread the Spores :) ==
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Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,391
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 15 hours, 6 minutes
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Re: Microwave sterilization good/bad? [Re: MAIA]
#322440 - 05/21/01 01:24 PM (22 years, 4 months ago) |
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Log in to view attachment
Trying pic again.
See'ya.
== :) Spread the Spores :) ==
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Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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cbee
journeyman
Registered: 04/26/01
Posts: 49
Last seen: 21 years, 6 months
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Re: Microwave sterilization good/bad? [Re: MAIA]
#322534 - 05/21/01 03:10 PM (22 years, 4 months ago) |
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can u use microwave sterilization to sterilize substrate rye jars?
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 <-- he's happy cause he got laid last night...
Visit the Free Spore Ring.. support the happy face
http://go.to/FreeSporeRing
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Aleon_Niir
Stranger
Registered: 05/20/01
Posts: 5
Loc: South America/Scandinavia
Last seen: 22 years, 1 month
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 Growing in honeywater? [Re: MAIA]
#322560 - 05/21/01 03:48 PM (22 years, 4 months ago) |
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How does it work to grow in honeywater? Are there any guids of it? What are the opinios of you who have tried it?
I would like to know everything about the honywater method if it?s good.
/Aleon Niir
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/Aleon Niir
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Teragon
Noddy
Registered: 02/20/01
Posts: 36,253
Loc: Lost in the Patterns
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Re: Microwave sterilization good/bad? [Re: Great_Cthulhu]
#322589 - 05/21/01 04:17 PM (22 years, 4 months ago) |
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I just recently sterilized two Ziploc containers in the microwave, PF style. I then innoculated them, and now they show great signs of strong mycelium growth. I sterilized them in the microwave for 8 mins on high, sitting in a layer of water about 5 mm deep. I also boiled water in a teapot and poured it into a mug when it was steaming and set that into the microwave so that the containers wouldn't lose any moisture. The tops popped off during sterilization, but I pushed them back on, and taped up the holes before I took it from the microwave to avoid contams. Let it cool down(overnight), and then innoculate. Make sure you put holes in the top before putting in the microwave. Enjoy!
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need that cash to feed them jones.
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cbee
journeyman
Registered: 04/26/01
Posts: 49
Last seen: 21 years, 6 months
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Re: Microwave sterilization good/bad? [Re: Teragon]
#322994 - 05/21/01 11:52 PM (22 years, 4 months ago) |
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so can u use it to sterilize grains like rye, bird seed, etc?
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<-- he's happy cause he got laid last night...
Visit the Free Spore Ring.. support the happy face
http://go.to/FreeSporeRing
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,391
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 15 hours, 6 minutes
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Re: Growing in honeywater? [Re: Aleon_Niir]
#323331 - 05/22/01 12:54 PM (22 years, 4 months ago) |
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"I" did it this way:
1- Get a 250ml jar with plastic lid, punch a hole on lid and tape it.
2- Fill the jar with destiled water
3- Put 1/2 tea spoon with honey into the jar and mix it.
4- Microwave with it for 5 minutes, max power.
5- Take jar, put it inside freezer and let it freeze (it takes some time depending on freezer).
6- Take jar out of freezer and put it inside some container with water for 5 minutes to defreeze the glass.
7- Microwave it again (5 min) and then let it rest untill it's cold.
8- Grab spore syringe and inoculate with 1cc using the hole on lid.
9- Let jar rest on a heating pad at 75F or 80F.
Steps 5,6 and 7 are optional, i use this freezer technique when microwaving just to reduce contam. probabilities.
After 3 days you'll see mycelium growing inside the jar.
Good luck.
Cheers,
MAIA
== :) Spread the Spores :) ==
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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