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Offlineeffwun
supafly guy
Registered: 10/04/01
Posts: 27
Last seen: 13 years, 8 months
Honeywater failure
    #424020 - 10/13/01 12:01 PM (16 years, 2 months ago)

here's what my friend did. he followed the honeywater tek pretty much exactly. he used 1 tsp of the lightest honey he could find at the local health food store and used bottles water to add. cooked at 10 psi for 30 mins. inoculated with PF original. gently shook it every couple days and after 5 or 6 days it got cloudy and he could see little bits of what he assumed was mycelium. he also wanted me to note that he used filter discs. he was extremely sterile using lysol & a hepa filter too. anyways, when he used the honeywater to inoculate rye, nothing happened. btw he used a sterile (pressure cooked) syringe to transfer the honeywater. does anyone have an idea what went wrong?


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: Honeywater failure [Re: effwun]
    #424202 - 10/13/01 04:13 PM (16 years, 2 months ago)

It may be you didn't wait long enough. I've made that mistake.


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InvisibleFungusFarmer
old hand

Registered: 09/20/00
Posts: 260
Re: Honeywater failure [Re: puscle]
    #424412 - 10/13/01 08:15 PM (16 years, 2 months ago)

Give it more than 5-6 days. After the water starts to get cloudy, wait another week or so. Sounds like it was just getting started

Grover


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-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- "What did you dream...it's all right we told you what to dream! Welcome, my son, to The Machine" Roger Waters, Pink Floyd


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Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 15 years, 7 months
Re: Honeywater failure [Re: FungusFarmer]
    #424708 - 10/14/01 01:51 AM (16 years, 2 months ago)

Honeywater has a high failure rate. When everything works properly, the mycelium should look like bits of cotton floating in an otherwise perfectly clear liquid. A cloudy medium is a sure sign of contams. Even a perfectly good looking batch can have hidden contams that take off when you inoculate grain. It sounds like you did everything right but honeywater requires good technique and no small amount of luck. My guess is that you have a bacterial contam on the grain that has lysed (dissolved) the shroom mycelium. Time will tell.


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InvisibleFungusFarmer
old hand

Registered: 09/20/00
Posts: 260
Re: Honeywater failure [Re: sylo]
    #424736 - 10/14/01 02:31 AM (16 years, 2 months ago)

Sylo I respectfully disagree.

In my experience, the water ALWAYS becomes cloudy a few days before real growth starts. This is completely normal, and if you would wait a few more days you'd probably have perfectly healthy mycelium

Honey water is only as difficult as your sterilization tek. I've had great success boiling the water for 3 minutes, then covering with seran wrap and a rubber band. even jars that have been left alone for several weeks still innoc just fine, even without injecting h202 occasionally.

Sounds like you've had bad HW experiences, what method did you use?

Grover


--------------------
-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- "What did you dream...it's all right we told you what to dream! Welcome, my son, to The Machine" Roger Waters, Pink Floyd


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