Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Myyco.com Pan Cyan Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Jars - Bad?
    #313311 -

Simple mix, still a relative newbie (Made about 3 batches so far, all good)...
Verm + BRF (Mix is the same as previous batches)
8 x 1/2 pint wide jars (Prev used /2 pint tall jars)
Purchased Syringe, 10cc Cyan (Prev own syringes, B+, using 5 x 3cc Syringes)
Steralized via pressure cooker - 1.5 hours @ 15 PSI (prev boiled for 2 hours)
Innoc @ 4 points / jar, 1 cc/jar (Prev 3cc/jar over 4 points)
Flamed the tip between jars, shot out excess till it stop steaming, + another 5 drops. On 4 jars, held tip in oven to reduce contam and waited an extra 10 seconds before injecting, to ensure it was cool enough. Oven-rack method to maintain sterility. First time using Ramsey seal.

3 weeks since batch made. No mycel showing whatsoever. Bad syringe? Non-sterile? Bad ramsey seal? Just a bad batch? How / Where did I possibly screw up? I'd like to figure out what happened before I try to correct via new batch.

---------------------------------------

There are three types of people in this world:
Those who can count, and those who can't.


--------------------
------------------------------------------------------
There are three types of people in this world:
Those who can count, and those who can't.

Extras: Filter Print Post Top
Re: Jars - Bad? [Re: Freezen]
    #313369 -

If it was a case of non-sterility, you'd see or smell something growing by now.

How does the substrate look? Is it wet or slimy looking?
Was your BRF organic?
Incubation temperature?
How do you take temperature measurments?
Did you use tap water?
Did you say that was a Pan cyan syringe?



--------------------
Where there's skill, there's a better way..

Extras: Filter Print Post Top
Re: Jars - Bad? [Re: egghead]
    #313387 -

Or you might have baked them dry. 1 and 1/2 hours in a pressure cooker?


Extras: Filter Print Post Top
Re: Jars - Bad? [Re: egghead]
    #313388 -

Substrate looks a little slimy, but I assumed that was normal... Same recipe. I would assume it would be drier than the first batch, as I boiled them to steralize WITHOUT a ramsey seal (just covered with tinfoil and held with elastic)

BRF, unpasteurized... This time I used bulk unpast. BRF instead of packaged (Cost was WAY cheaper, 1.90 for 1200mg, instead of 3.99 for 300mg)

Incubation was about the same (70-80), same environment, elec. thermometer, tap water both times...

Psilocybe cyanescens... Before made own syringes from a B+ print....

There are three types of people in this world:
Those who can count, and those who can't.


--------------------
------------------------------------------------------
There are three types of people in this world:
Those who can count, and those who can't.

Extras: Filter Print Post Top
Re: Jars - Bad? [Re: Freezen]
    #313394 -

Pressure cooker had 1L of water in it, according to instructions from it (Just bought it)... would that still have dried them out?

There are three types of people in this world:
Those who can count, and those who can't.


--------------------
------------------------------------------------------
There are three types of people in this world:
Those who can count, and those who can't.

Extras: Filter Print Post Top
Re: Jars - Bad? [Re: Freezen]
    #313402 -

sometimes there's this thing called "being TOO sterile" ... if you just got a pressure cooker i hope you know how to use it ... but steaming will do the same job :tongue::laugh:... anyway ... dont flame your needles and cool them with the stuff that's inside of the syringe! dip it in some pre-boiled water that you allowed to cool .. if you have it in a cup it should make a ringing sound when you plunge the red hot needle in the water... this ringing tells you your needle is cool .... i'm not saying all that flaming is wrong it all depends on how paranoid/careful you are about contams- some monkeys flame the needle 1ce before innoculating up to 8 jars (or however many jars they happen to sqeeze out of 1 syringe...) .. anyway i hope i helped a little .. peace and good luck

go fish

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Myyco.com Pan Cyan Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* black spots in jars bad? :o( shroomme 4,187 14 10/11/01 03:51 PM
by honeyroasted
* Re: colonized jars & bad timing Lizard King 1,200 2 12/07/99 08:31 PM
by Lizard King
* First Time Grower: Day 16 : Grain Jar Transfer Questions, 'Howmidoin?' Gordon Shumway 1,340 8 05/07/21 05:39 PM
by Gordon Shumway
* Shakin' a bunch of jars....Too many jars...
( 1 2 3 all )		 Justweed 2,295 50 06/03/21 07:42 PM
by Mr. Mushie
* Do I need to PC bacterial jars or is that just for moldy jars? *DELETED* ButtFace 928 15 05/06/21 06:00 AM
by Smartattack
* unpasteurized coir performs poorly PocketRevolution 821 0 02/10/10 04:39 PM
by PocketRevolution
* Is this healthy? Current concerns. PF Tek Jars frenzylock 5,155 17 05/06/21 05:44 PM
by Ashtray161
* Stamets recommends unpasteurized casing? MasFina 1,822 6 02/27/07 12:17 AM
by Blutjager

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,439 topic views. 11 members, 255 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 16 queries.