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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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How long is a spore print viable?
    #29726195 -

I have sport prints that I have never opened and never swapped that are between 5 and 7 years old. Do you think they're still viable?


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et in arcadia, ego

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Re: How long is a spore print viable? [Re: Vulcan_dikc] * 1
    #29726202 -

Depends if they were stored correctly in cold storage.

I still germinate spores from the early 2000s because they've always been stored in the fridge.

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Re: How long is a spore print viable? [Re: Vulcan_dikc]
    #29726203 -

Could still be viable.

From my understanding it's a slow gradual decline as individual spores stop being viable. The older the print, the less viable spores there are. It's not like a hard cut-off.

Making your agar a bit on the soft side can help stubborn spores germinate. BRF pucks work well too.

Good luck!


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: How long is a spore print viable? [Re: Vulcan_dikc]
    #29726280 -

Hydrating old spores with a surfactant like Tween 20 can also help to germinate. I’ve had spores in serial dilution germinate due to Tween 20 before. It only takes a drop or two in a 10ml syringe to encourage them.

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Re: How long is a spore print viable? [Re: MomoMyco]
    #29726504 -

Thanks, folks!


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et in arcadia, ego

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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