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I've had many good runs but no more? * 2
    #29719149 -

I struggled for like a year learning the process then for the last 1.5 years or so I've been popping shrooms from cakes like a champ. Now it seems every wedge I drop just contams! The fuck?
Either that or it just lies there in the popcorn dormant. Can one lose their blue thumb that fast??

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Re: I've had many good runs but no more? [Re: Shroomhead2] * 3
    #29719161 -

Yes it can happen, how fast and severe that is depends on the workflow and
how you keep up your library of genetics, how many multiple lineages of the
same master culture (as in proven clean culture) you run or if you even
have masters or some sort of library of genetics you keep as backup.

It's crucial to have at least a basic system and damage control in place.

It's like a video game where you want to use a save point so you don't have
to start from scratch if you die.

Contam ain't sleep

Edited by Baba Yaga (11/04/26 04:51 PM)

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Re: I've had many good runs but no more? [Re: Baba Yaga]
    #29719247 -

Been cloning from the same batches a lot. And haven't rose many strains from spores. It is ALL coming from one syringe I bought like over a year ago. Is it senesence already???

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Re: I've had many good runs but no more? [Re: Shroomhead2]
    #29719248 -

Oh the last two batches of popcorn I sterilized kinda scorched at the bottom. Is that a problem? I dont know if its any different from having grill marks on a burger. Sterile is sterile right even if some grains are burnt at the bottom of the jar. I don't know why suddenly there scorching is it time for a new PC? And I also been using some cheap small white kernel popcorn instead of the fatter yellow ones.

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Re: I've had many good runs but no more? [Re: Shroomhead2] * 4
    #29719251 -

Shroomhead2 said:
Been cloning from the same batches a lot. And haven't rose many strains from spores. It is ALL coming from one syringe I bought like over a year ago. Is it senesence already???



No.
Sounds like you need to go back over things , stop scorching grains yes sterile is sterile but dry is also dry which is bad for your grain and your pc


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: I've had many good runs but no more? [Re: cronicr] * 3
    #29719259 -

Moisture content of grains is important to the sterilization process. Mold is killed quickly, but a complete PC cycle is needed to kill all bacteria. Bacterial spawn weakens the mycelium, which gives decomposing molds like Trich a foothold.

If your spawn is burning at the bottom, it might be drying out and not being completely sterilized.

What PC are you using? Is it running dry at the end of the session? Are you using a trivet or something to separate the glass from the heat?

Also, pics of your agar and spawn would help us diagnose the issues.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: I've had many good runs but no more? [Re: Shroomhead2] * 1
    #29719334 -

Shroomhead2 said:
Oh the last two batches of popcorn I sterilized kinda scorched at the bottom. Is that a problem? I dont know if its any different from having grill marks on a burger. Sterile is sterile right even if some grains are burnt at the bottom of the jar. I don't know why suddenly there scorching is it time for a new PC? And I also been using some cheap small white kernel popcorn instead of the fatter yellow ones.



Not guaranteed to be a problem, but maybe a bit weird. I tend to overcook my popcorn and while things might darken a bit, any kind of scorching is pretty far out.

Small white kernel is fine, maybe even better. I've gone back and forth between colors and sizes, they all work.

What's your grain prep like? Are you simmering until the centers are cooked through? The smaller kernels might dry out sooner maybe?

Overdry grains can definitely contribute to lackluster/dormant growth. Been trying millet lately and really getting that hammered home over and over lol.

After 1.5 years some weakening can be expected but nothing grow-killing. They can stay strong longer than that.

Try a start from spore, or on some different grains, see how things switch up maybe


--------------------


.~`  ๐”… ๐”ž ๐”ค ๐”ข ๐”ฉ  ๐Ÿฅฏ  ๐”… ๐”ฆ ๐”ฑ ๐”ข ๐”ฐ  `~.
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๐Ÿงช Castellani Preservation | ๐Ÿ”ฌ The Slidewich | โ˜ ๏ธ Touchless skull sterilizer | ๐Ÿ“ธ Agar plate scanner


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Re: I've had many good runs but no more? [Re: Shroomhead2] * 1
    #29719336 -

Shroomhead2 said:
Been cloning from the same batches a lot. And haven't rose many strains from spores. It is ALL coming from one syringe I bought like over a year ago. Is it senesence already???



Are you saying that youโ€™re cloning clones? If so itโ€™s not a great plan. Agree with everything everyone else mentioned but if youโ€™re expanding by cloning clones repeatedly you just indeed may be having some senescence at play as well. You have a range of vectors right now, definitely need to tighten up if you want to run working culture continuously.

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Re: I've had many good runs but no more? [Re: Pastywhyte] * 1
    #29719341 -

My wild-ass guess would be that if we're talking about a bunch of lines all originating from the same syringe, and clones are being taken from fruits grown in open air, that there's just a massive bacterial bottleneck forming.


--------------------


ACAB  ๐Ÿ–•๐ŸงŠ


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Re: I've had many good runs but no more? [Re: B Traven] * 2
    #29719343 -

Popcorn was also a pretty sus grain for me. I had several bacterial blooms just for popcorn being annoying to hydrate properly and it has a huge R value due to its size. Takes more time to properly sterilize compared to other grains.

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Re: I've had many good runs but no more? [Re: Pastywhyte] * 2
    #29719567 -

Happy bday ya ole fuck


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: I've had many good runs but no more? [Re: cronicr] * 4
    #29719691 -

Thanks cron, I am super old now, hard to believe I made it this far :oldman:

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Re: I've had many good runs but no more? [Re: Pastywhyte] * 2
    #29719926 -

Pastywhyte said:
Thanks cron, I am super old now, hard to believe I made it this far :oldman:



Happy bday Paste-Man





--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : โ™พ๏ธ : MY FAV TEKS ๐Ÿ“–

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Re: I've had many good runs but no more? [Re: Shroomhead2]
    #29719997 -

I am currently having a large spike in contamination rates as well, in my case it was because I changed my workflows without properly preparing. Upgraded from a SAB to an FFU, but I didn't rehearse the modified workflows until I was comfortable with them and there edge cases as I had with my SAB.


I would recommend identifying changes in your workflows and training on them until you are comfortable. Document mistakes you identify in the dry runs, when you are comfortable to go back in with materials annotate any deviations so a contamination vector can be identified if it occurs.


--------------------
Read the guide, read it again, understand the guide, then start the hobby.

I have no idea what I am doing, but I'm having fun doing it!

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Re: I've had many good runs but no more? [Re: Shroomhead2] * 5
    #29720267 -

Youve gotten a lot of good advice here. I'd like to add, just keep cultivating... I mean seriously, don't ever give up. I fully understand the frustration here, I really do, this summer I threw away more grain and tubs than I had successful grows. It was brutal. But as I kept at it, certain things began to stand out to me. Things that should have been obvious came to light.

Now I can happily say, a majority of my grows turn out fine. There's so much to learn with this craft. Think over every variable, of every step you make. It'll come together and you'll be a better cultivator at the end of the day for it:smile::mushroom2:

Best of luck! Hope to see you around for a long time :hug:

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Re: I've had many good runs but no more? [Re: Agar Wedge] * 3
    #29720753 -

Hey, I can join in the choir of having tons of contam when my first few grows literally were perfect...

Check out this shizzle:







This is just 3 pics out of countless ones of contam

Shall I give up cuz I got contam (which happens to everyone)?

Fuck To the hell No I will not give up

All failures are simply learning lessons in disguise

Tighten up thine process and grow more medicine than you can consume in a lifetime and then you can get to the point where you just grow for fun like Fahster

Keep calm and grow on

:hatsoff:


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : โ™พ๏ธ : MY FAV TEKS ๐Ÿ“–

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Re: I've had many good runs but no more? [Re: langten69]
    #29720785 -

langten69 said:
Hey, I can join in the choir of having tons of contam when my first few grows literally were perfect...

Check out this shizzle:







This is just 3 pics out of countless ones of contam

Shall I give up cuz I got contam (which happens to everyone)?

Fuck To the hell No I will not give up

All failures are simply learning lessons in disguise

Tighten up thine process and grow more medicine than you can consume in a lifetime and then you can get to the point where you just grow for fun like Fahster

Keep calm and grow on

:hatsoff:



Awesome attitude.  And I've been there too--just recently.  Damn trich factory.  Forced me to really tighten up my game (after getting totally pissed off from tossing shoeboxes)


--------------------
Need genetics? Hit me up!


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Re: I've had many good runs but no more? [Re: Nerdo]
    #29720797 -

I see trich factories as just a catalyst for us as growers to actually get our shiz together. After enough fails, its like eff this, then you just get so locked in that all contam gets erradicated in the process:rockon:


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : โ™พ๏ธ : MY FAV TEKS ๐Ÿ“–

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Re: I've had many good runs but no more? [Re: langten69]
    #29720822 -

langten69 said:
I see trich factories as just a catalyst for us as growers to actually get our shiz together. After enough fails, its like eff this, then you just get so locked in that all contam gets erradicated in the process:rockon:



I'm right there with ya.  Trich is our bitchy green teacher.


--------------------
Need genetics? Hit me up!


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Re: I've had many good runs but no more? [Re: Pastywhyte]
    #29722707 -

Happy Belated B-DaY! :awesomenod: :birthday:

Yeah, I would stay away from popcorn grain. There are so many better options out there. Leave the popcorn for movie night :sadyes:


--------------------
"A single lifetime in this density can accomplish a refinement of Will that might take far longer in a realm where the truth is always visible" - Zii, Confederation of Planets, GFL Station.

"You will then have national and global contact around 2026." - Pleiadian Prophecy 2020

PROJECT BLUE BOOK ANALYSIS (PDF Download) - 312 pages! | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms:shroomeryhead:Canned Computer Duster SAB Tek ๐Ÿ‘จโ€๐Ÿ”ฌ | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits | R.I.P. Big Worm :tombstone: || The Start of the Ascension Process was 2020. ๐ŸŒŽ๐ŸŒ๐ŸŒ                                                         
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