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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc]
    #29695451 -

:takingnotes: ahhhhh ok

Unheated dehydration = room temp dehydration

Thank Rev! :thumbup:

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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc] * 1
    #29703766 -

Uzi said:
Quote:
I will be testing it again in the next couple of days which will be over 350 days since dehydration. So far, I would call it very stable.



Ooh please do come back with an update. If dehydrated tea is stable up to a year like that, looks like youve found one of the best forms of storage for the goodies.

I was pretty happy with the volume reduction, and it seems like a good method to not have a bunch of jars with fruits laying around if you like to grow frequently for fun lol.



ReverendMyc said:
ReverendMyc said:
ReverendMyc said:
stasis said:
Hi Rev,

Do you have any initial ideas/anecdotal thoughts on potency/time for the dehydrated tea? I only recently made some out of dried suptropicalis and have found it to be a fantastic method thats very versatile. I was hoping to use it as the main storage method moving forward if it lasts the test of time.



ReverendMyc said:
I have tests planned. I was hoping that I had a sample that I had accurately measured and logged to do a retest to check for potency. Unfortunately, I have either used what I had in gummies or just combined various batches into a collection jar, so I don't have a good reference base value to retest.

I am going to prepare a batch of the dehydrated tea this week specifically to test. I will test its potency right after prep and then again a few times over the coming months to see how quickly it drops or if it stay roughly the same over time. So I will share some answers to that, but it will take some time for results to come in.



Crystalized Tea over time results.

After 55 days, dehydrated tea shows no loss in potency at all. The dried tea was made specifically for this test and stored in a quart canning jar with regular two piece lid. No vacuum was applied as I usually do. Not even kept in the dark. Just a regular jar with dried tea on a shelf in my lab.



I will continue to test this at various intervals. Most likely on the same days as the 5 year myceliated grain tests. 55 days and one test is not conclusive or anything, but it is a pretty encouraging place to start.



Day 107 and dehydrated tea in sealed jar still holding the same potency. Just over 2% with no vacuum or silica nor O2 absorbers. Just brown crystals in a clear jar on a shelf.

I will skip the next one and run another at around 200 days from dehydration and jarring.



Day 203 since first test. Potency still just over 2% actives. Thinking that it is pretty safe to say that the crystals hold their potency if kept dry. I will keep testing at long intervals when I test other things. See you at day 300~ish.



Tested again at day 356 and the results are just below 2%. The results show a possible loss of 3.35% potency after 1 year of storage. That is still within the 5% tolerance of the testing equipment, so may not be significant.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc] * 1
    #29703783 -

Amazing Rev. Thank you for your dedication

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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc] * 5
    #29703791 -

Double post because I also tested my latest method of tea making and it showed some significant improvements.

  • This time I did a vacuum extraction of 509 grams of fresh subtropicalis.
  • I used three decoctions using 500 ml of preboiled and cooled filtered water for each decoction.
  • I added approximately 1 gram of ascorbic acid powder to each 500ml decoction.
  • I drew a vacuum on 2000 ml canning jars using an electric vacuum pump.
  • This time I used 5-Pack 5 Gallon Reusable Mesh Filter Bags 5 Color Nylon Filter Bags 25 to 220 Micron Suitable for Hydroponics Gardening and Industrial Applications in succession to filter out the solids. This was far easier and more effective than all prior methods I have tried. Next time I may run the final tea from the 25 micron bag through a Aeropress to see if I can reduce the solids any further.
  • Then I dried the tea to crystals at 71c for 48 hours in small silicone cups.
  • The final weight of the dried tea was 20 grams which is a 96% reduction from fresh to dried tea.
  • Multiple samples of the dried tea were tested using the qtest and colorimeter with results around 45mg/g of dried tea.


This has been the best reduction and potency result so far. The crystals are a bit more powdery and a more redish brown than other runs. I am looking forward to seeing how much I can stuff into a 00 capsule and how gummies turn out.


Looks a bit more like I picture melange/spice.

Edited by ReverendMyc (10/20/26 03:25 PM)

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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc]
    #29704259 -

That's awesome Rev! Is your vacuum pump one for jars, or something stronger?


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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: bluescreen]
    #29704646 -

ReverendMyc said:
Quote:
Do you guys think that a manual vacuum pump as the one one uses to suck air from a opened wine bottle with the dedicated cork works as well as that electric device you use (reference appreciated aswell) ?

This is the kind of manual vacuum pump im talking about : https://cdn.etrias.nl/media/cache/product_lg/8/7/8714793098121.jpg
Apparently it achieves -5 psi or 500 Torr.



Before I got the electric vacuum thingy I was using one like this.


With this attacment for jars.


That worked fine and drew a vacuum just as well if you don't mind a little mini-workout for each use. :muscles: So, surely the wine thing should be similar if you can find the proper attachment... or you use wine bottles instead of canning jars. That is probably what you meant and I just realized it.

Here is an amazon link to the one I have now, though. They are relatively inexpensive if you are in the market. Mine has lasted for years of regular use.

Quote:
Also, I just read that soaking the biomass in water 30min before applying the vacuum could help the extraction...what do you think ? Potential psilocybin degradation ?



With fresh materials, I don't think that a soak will matter because the mush is already 90%+ water, but that is just a guess. If you grind up your fresh I would worry about oxidation of psilocin but with whole mushrooms or sclerotia I doubt soaking would do any help or harm as long as the cell walls remain in tact.

For dried mush materials, you are mostly working with psilocybin and not starting with much psilocin so a short soak is not likely to do much harm but I wouldn't go for a long soak. In my experience with gummy recipes, letting dried mush tea sit over night in a refrigerator caused a significant loss in potency.

Quote:
Such an easy way to make an extract in the wild...



I may be easy, but I am also cheap. Or something like that.

I am glad to hear if what I share is helpful. I appreciate you taking the time. Stick around, this place is full of good information and helpful souls in an old wild west sort of way.




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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: Tri]
    #29704857 -

Well putting a material like dryed tea extract in a fridge which pulls moisture onto everything will cause obvious degradation in my eyes. I've heard the tea extracts for gummies is fairly shelf stable at room temps for a year+ :amanita2:


--------------------





An enigma some caerulescens n a mono 😎

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Re: Top Tea Prep method test per potency testing of dregs, teas, & extracts [Re: ReverendMyc]
    #29706207 -

Quote:
  • This time I did a vacuum extraction of 509 grams of fresh subtropicalis.
  • I used three decoctions using 500 ml of preboiled and cooled filtered water for each decoction.
  • I added approximately 1 gram of ascorbic acid powder to each 500ml decoction.
  • I drew a vacuum on 2000 ml canning jars using an electric vacuum pump.
  • This time I used 5-Pack 5 Gallon Reusable Mesh Filter Bags 5 Color Nylon Filter Bags 25 to 220 Micron Suitable for Hydroponics Gardening and Industrial Applications in succession to filter out the solids. This was far easier and more effective than all prior methods I have tried. Next time I may run the final tea from the 25 micron bag through a Aeropress to see if I can reduce the solids any further.
  • Then I dried the tea to crystals at 71c for 48 hours in small silicone cups.
  • The final weight of the dried tea was 20 grams which is a 96% reduction from fresh to dried tea.
  • Multiple samples of the dried tea were tested using the qtest and colorimeter with results around 45mg/g of dried tea.





Do you think you could go into this new method in a bit more detail when you get the time, Rev? It seems pretty straightforward but theres a few things im not understanding fully plus a few changes in the results from your last method id love to know more about or just pitch a thought about.

A little more clarification would be great for:

1. Boil then cool water to room temp (why not just skip the boil if its gonna cool?) If you did three separate decoctions could you also just do one single decoction with the same amount of water but combined?

2. Put it in a jar along with the fruits, ascorbic acid, and vacuum it with that electric jar vac (for how long under vacuum? Do you aggitate or use a stir bar or just let it sit) Does the pH really seem to make much a difference? could you use another acid in place of ascorbic like food grade malic or citric?

The results seem great though, great work man.

Another thing i wanted to mention was the finished products weight compared to the weight of the starting product plus the potency.

In your last method and when we spoke before you said that you would end up with the exact same weight in dried tea as the final product and that the potency didnt change at all from the starting material, just a reduction in volume. Here if you were to have dehydrated the fruits youd get somewhere around 50g of dried from the 509g wet you got so from your previous method one could assume youd end up with 50.9g of dried tea with the same potency as before, but it seems this time you did actually lose the weight (reduced by 60% if going by dry weight conversion) and that the potency was definitely concentrated (aint no way those fruits hittn 45mg/g right?). Does that seem to track with what youre getting? Sorry for the long post, I do my own tests as well I just dont have access to the miraculix tests atm to confirm potency with accuracy.

PS: turns out that batch i mentioned a few weeks ago where i reduced it down with a hard boil for a while in basically entirely inactive so uhh, yeah down boil your tea for an extended period of time lol, can definitely cross that method off the list.

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