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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Ninja contam!? * 2
    #29686671 -

I cooked these wheat berries per beef's wheat prep tek. I poked some holes in my jar lids, and rcv'd ez felt over the holes two days prior to prepping the wheat. I dried the grains in the tumble dryer, and then sterilized them in my presto PC.

Quote:
skrimplet said in her journal:

After having pressure cooked my grain jars for nearly 120 minutes, I saw that the pressure had dipped to about 13 PSI for a few minutes. I brought it back up to pressure and let it cook for roughly another 45 minutes.


1 jar was noc'd up 2 days later. She's looking a little dirty (spiky myc, sweaty jar, uncolonized spots pressed against glass) but nearly done colonizing. 6 jars are currently looking sweaty but the grain is still free moving. 4 of them are.... well, see for urself

#1











#2 I did not noc this jar, and it shows the exact growth pattern from a plate of white mold that I let grow for science.












#3










#4










Can anybody enlighten me as to what the fuck might be going on here? Feeling butthurt :snub:


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

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Re: Ninja contam!? [Re: skrimplet]
    #29686676 -

What did you use as an inoculant? Also what do you mean by tumble dry


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ninja contam!? [Re: PuffM4gicDr4gon]
    #29686680 -

Edited to add: NONE of these jars were noc'd. They were PC'd and stored. That white one is straight mold: I had 0 desired spores in my apartment at the time of prepping these.

I used agar. Also pls don't flame me for this, I'm a busy woman with a small apartment But I put the grain in a mesh bag, and the mesh nag in a pillow case, and I dried it in my clothes dryer. The grain prep process is in my journal called "arthur," if it will help elucidate it.


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

Edited by skrimplet (10/05/26 02:06 PM)

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Re: Ninja contam!? [Re: skrimplet]
    #29686689 -

So are you asking what type of contam it is since you didnt innoc it with mycelium?


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"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍶*underconstruction*
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION 🧪
JOIN CAKE WARS III TODAY 🫵
MODERN FRUITING CONDITIONS 🍄
REVITALIZE THE CUBENSIS GENEPOOL 🥊
MY FAVORITE TEKS 📖

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Re: Ninja contam!? [Re: skrimplet] * 1
    #29686691 -

No one's going to bash or flame you for trying to grow mushrooms, Skrimp.

Those jars are heavily contaminated. Something is way off with your filters I guess. If you PCd those jars and pressure dropped a little around the two hour mark, that in no way explains the mold in your jar. Mold spores don't need much heat to die. Jar no. 3 is bacterial, I mean that is a very real possibility if your cycle was too short / pressure wasn't reached at all points during the cycle etc. But that mold would've been introduced after PCing.


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Community is resistance

Resistance is existence

Species grown so far

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Re: Ninja contam!? [Re: skrimplet]
    #29686693 -

Mold is usually pretty quick to die in a pressure cooker. Generally somewhere around 100c kills most mold.

Just to confirm you didn't use agar on these jars at all? You just pressure cook them and left them somewhere?

If that is the case then I would look at your grain prep and pressure cooking process.

I haven't heard of anyone using a dryer, if it dried out the grains too much then that might cause problems.

What are you using as a pressure cooker and can you tell me about your process with it?


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: PuffM4gicDr4gon]
    #29686695 -

Tao is right about the filters as well. Can we get a picture of them?


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: PuffM4gicDr4gon]
    #29686722 -

The tumble dryer :grin: I guess it can work. Why not? It’s pretty hard to over-dry properly hydrated grains, and I think you would need to put it in a tumble dryer for a long time before it’s too dry to be properly sterilised. The mould wouldn’t survive 2 hours in your pressure cooker at 15psi even if the grain wasn’t hydrated at all.

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Re: Ninja contam!? [Re: ambigu] * 1
    #29686731 -

here's my filters:





Puff, Im using a 23qt presto pressure canner. I follow Bod's pc tek to the T except for when I let it go under pressure :facepalm:

Langten, mostly I was wondering if it was more likely to be my prep or my filters. Since my grain was not over dried (just dry on the outside but still hydrated), and I PC'd it for damn near 3 hours, I guess I can only assume it's my filters. Thanks guys 😔 back to the drawing board


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

Edited by skrimplet (10/05/26 02:50 PM)

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Re: Ninja contam!? [Re: ambigu] * 2
    #29686736 -

It's good that you let a few uninoculated jars ride for a few days. Definitely the best way to tell whether your PC cycle is working or not.

120 mins for a grain jar that size should be adequate, even with a brief dip to 13 PSI, and even if you didn't do the 10 min steam vent before capping with the PC weight.

The grain prep doesn't matter here. You could store them outside in a bucket of rain and sewage, throw them in soaking wet, and a proper PC cycle would still keep them squeaky clean for eternity.

Quote:
rcv'd ez felt over the holes


This is almost certainly the issue.

Contaminants we care about are typically in the one to three micron range. I don't know if that felt is rated at the micron level at all, but my guess is probably not. A few single-micron particles falling through the air between felt fibers seems easy to imagine.

If you want to isolate the variables some more, do another run with some felt lids and some unmodified lids, and let them go uninoculated for awhile. If the unmodified lids stay clean, felt's your issue.

But I wouldn't bother. These stick-on filter discs will sort you out for $8-10. Just one on the outside over the hole is plenty, I don't RTV them on or anything.


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.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III HAPPENING NOW :salute:

🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


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Re: Ninja contam!? [Re: Rasmodius]
    #29686743 -

Thanks Ras. I was trying to save a quick buck but I ended up wasting -_- at least I have felt for arts and crafts.

Also, is it true that you cucked Pierre?


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: skrimplet]
    #29686749 -

skrimplet said:
Thanks Ras. I was trying to save a quick buck but I ended up wasting -_- at least I have felt for arts and crafts.

Also, is it true that you cucked Pierre?


We just... don't talk about Abigail.

Sure is a coincidence she's such a bad bitch tho. How 'bout that. Had to get it somewhere

:douchewink:

Lotta ways to end up divorced in this valley :lol:


--------------------


.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III HAPPENING NOW :salute:

🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


Extras: Filter Print Post Top
Re: Ninja contam!? [Re: Rasmodius] * 1
    #29686754 -

I use dumpsterdived polyfill, zero extra environmental impact, zero costs, works like a charm.

If you're into arts and crafts, check Dala's thread (Dala's Tie Dye Porn, I still don't seem to be able to post a link).


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Community is resistance

Resistance is existence

Species grown so far

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Re: Ninja contam!? [Re: Rasmodius]
    #29686768 -

How old is your PC? If the rubber seal is starting to get brittle and not seal properly then the PC won't hold the correct PSI to sterilize. You'd know if the seal was going because it drips a lot of water and/or pisses steam out the sides once it gets to pressurizing.


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Re: Ninja contam!? [Re: thumbsy]
    #29686785 -

It's the felt.

I'm still trying to find that quote... Oops... right there in OP.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Ninja contam!? [Re: DV8DUG]
    #29687408 -

DV8DUG said:
It's the felt.

I'm still trying to find that quote... Oops... right there in OP.


find what quote...?


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: skrimplet]
    #29687684 -

You could try using unmodified one piece lids, all you have to do it get them just barely tight and then back the lid off like 1/8"-1/4", the slightly loose lid allows for GE, and it's pretty damn hard for a contam to work it's way vertically up the threads on the lid/jar. Just gotta remember to tighten the lid down before you handle the jar at all. I run all my grain jars this way, and also all of my Cake Wars 3 cakes are in 8oz jars with unmodified lids :rockon: just a suggestion!






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Re: Ninja contam!? [Re: fontinalis666]
    #29688475 -

thx font. spent all day running grain w new filters. Im worried it will just all contam again. This filter mishap has set me back 6 weeks, back at square one.


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idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: skrimplet] * 1
    #29688486 -

It happens to everyone every now and again.

I had to shut everything down and start over to track down some problems I was having. Just got back to tubs a couple weeks ago.

Keep at it and you'll get there. Once all the wrinkles in your process are worked out, you'll probably always have something going for every stage of growth and failure won't feel so crippling.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Extras: Filter Print Post Top
Re: Ninja contam!? [Re: PuffM4gicDr4gon]
    #29688869 -

Hope the new filter lids you made work out 🤞 but if not I'd def just go ahead and remove the filter from the equasion entirely


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